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Reverse genetics in virology: A double edged sword
Biosafety and Health, 2022 Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.
Hongyu Chen, Hongqi Liu, Xiaozhong Peng
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Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis
Viruses, 2021 Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto +3 more
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Reverse genetics in ecological research.
PLoS ONE, 2008 By precisely manipulating the expression of individual genetic elements thought to be important for ecological performance, reverse genetics has the potential to revolutionize plant ecology.
Jens Schwachtje +2 more
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RNA Virus Reverse Genetics and Vaccine Design
Viruses, 2014 RNA viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. The development of reverse genetics systems for manipulation and study of RNA virus genomes has provided platforms for designing and optimizing ...
Christopher C. Stobart, Martin L. Moore
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Reverse genetics in zebrafish by TILLING [PDF]
Briefings in Functional Genomics and Proteomics, 2008 TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for ...
Cecilia B, Moens +3 more
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Reverse genetics in eukaryotes [PDF]
Biology of the Cell, 2010 Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations.
Hardy, Serge +3 more
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Reverse Genetics System for Rabbit vesivirus
Frontiers in Microbiology, 2020 Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
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Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
Инфекция и иммунитет, 2023 Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of ...
N. V. Larionova +4 more
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Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System
mBio, 2022 Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans.
Avan Antia +2 more
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Marburg Virus Reverse Genetics Systems
Viruses, 2016 The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses.
Kristina Maria Schmidt, Elke Mühlberger
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