Reverse genetics in virology: A double edged sword
Biosafety and Health, 2022 Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.
Hongyu Chen, Hongqi Liu, Xiaozhong Peng
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Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis
Viruses, 2021 Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto +3 more
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Reverse genetics rescue of sylvatic dengue viruses [PDF]
Journal of VirologyThere are an unknown number of sylvatic (“sylvan” = “of the forest”) dengue viruses currently being sustained by nonhuman primates and mosquitoes in the forests of Africa and Asia.
Paul Gendler +6 more
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Engineering Nipah virus: Reverse genetics as a gateway to novel drug discovery [PDF]
New Microbes and New InfectionsNipah virus (NiV) is a highly pathogenic and re-emerging virus that requires containment in biosafety level 4 (BSL-4) laboratories. The limited accessibility of these high-security facilities poses major obstacles to investigating immunopathogenesis and ...
Muralidharan Menon Arjun +1 more
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RNA Virus Reverse Genetics and Vaccine Design
Viruses, 2014 RNA viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. The development of reverse genetics systems for manipulation and study of RNA virus genomes has provided platforms for designing and optimizing ...
Christopher C. Stobart, Martin L. Moore
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Research progress and applications of reverse genetics systems for infectious bronchitis virus [PDF]
Poultry ScienceInfectious bronchitis virus (IBV) poses a persistent threat to global poultry health, driving the need for advanced molecular tools to study and combat this pathogen.
Yinghao Zhao
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Highs and Lows in Calicivirus Reverse Genetics [PDF]
VirusesIn virology, the term reverse genetics refers to a set of methodologies in which changes are introduced into the viral genome and their effects on the generation of infectious viral progeny and their phenotypic features are assessed.
Ángel L. Álvarez +7 more
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Exploring enterovirus pathogenesis and cancer therapy potential through reverse genetics [PDF]
Biosafety and HealthOver the past decades, oncolytic viruses have emerged as prominent therapeutic agents with significant potential for anticancer treatment. Enteroviruses (EVs) have garnered particular attention due to their specific tropism for various types of tumor ...
Shijin Wang +6 more
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Identification of an optimal exogenous gene insertion site (P-M) and establishment of a reverse genetics system for aMPV/A. [PDF]
PLoS ONEAvian metapneumovirus subgroup A (aMPV/A) causes widespread infections in commercial poultry worldwide. The development of highly efficient viral vector vaccines is constrained by multiple factors, among which the lack of a mature reverse genetics system
Yu Guo +9 more
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Efficient Plasmid-Based Rescue of T7 RNA Polymerase-Driven Calicivirus Reverse Genetics Systems in Mammalian Cells Using Vaccinia Virus RNA-Capping Enzymes [PDF]
VirusesThe caliciviruses include important human and animal pathogens such as norovirus, sapovirus and feline calicivirus. Viral reverse genetics is performed to understand the fundamental biology of these viruses, as well as a potential route to generate live ...
Frazer J. T. Buchanan +7 more
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