Results 41 to 50 of about 335,945 (253)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

Phytopathology Research, 2020
Plant virus-induced diseases cause significant losses to agricultural crop production worldwide. Reverse genetics systems of plant viruses allow gene manipulation on viral genomes, which greatly facilitates studies of viral pathogenesis and interactions ...
Ying Zang, Xiao-Dong Fang, Ji-Hui Qiao, Qiang Gao, Xian-Bing Wang   +4 more
doaj   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

PLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu, Ying Shi, Dawei Yan, Xuesong Li, Pixi Yan, Xuyuan Gao, Yuee Zhang, Lei Yu, Chaochao Ren, Guoxin Li, Liping Yan, Qiaoyang Teng, Zejun Li   +12 more
doaj   +1 more source

Modern Approaches to Molecular Genetics of Viruses in the Study of the Members of the Family Coronaviridae

Вестник войск РХБ защиты, 2021
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation

Virology Journal
Influenza is a highly contagious acute viral illness that affects the respiratory system, posing a significant global public health concern. Influenza B virus (IBV) causes annual seasonal epidemics.
Ghayyas Ud Din, Chunchen Wu, Zahra Tariq, Kinza Hasham, Muhammad Nabeel Amjad, Bei Shen, Lihuan Yue, Muhammad Asif Raza, Muhammad Awais Ashraf, Lingdie Chen, Yihong Hu   +10 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Characterization of a human H3N8 influenza virusResearch in context

EBioMedicine
Summary: Background: In 2022 and 2023, novel reassortant H3N8 influenza viruses infected three people, marking the first human infections with viruses of this subtype.
Chunyang Gu, Shufang Fan, Randall Dahn, Lavanya Babujee, Shiho Chiba, Lizheng Guan, Tadashi Maemura, David Pattinson, Gabriele Neumann, Yoshihiro Kawaoka   +9 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Rescue of recent virulent and avirulent field strains of bluetongue virus by reverse genetics. [PDF]

PLoS ONE, 2012
Since 1998, Bluetongue virus (BTV)-serotypes 1, 2, 4, 9, and 16 have invaded European countries around the Mediterranean Basin. In 2006, a huge BT-outbreak started after incursion of BTV-serotype 8 (BTV8) in North-Western Europe.
René G P van Gennip, Sandra G P van de Water, Christiaan A Potgieter, Isabel M Wright, Daniel Veldman, Piet A van Rijn   +5 more
doaj   +1 more source