Results 61 to 70 of about 668,266 (290)

Reverse genetics of rotavirus [PDF]

Proceedings of the National Academy of Sciences, 2017
The genetics of viruses are determined by mutations of their nucleic acid. Mutations can occur spontaneously or be produced by physical or chemical means: for example, the application of different temperatures or mutagens (such as hydroxylamine, nitrous acid, or alkylating agents) that alter the nucleic acid.
openaire   +2 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Genome-wide association studies identify heavy metal ATPase3 as the primary determinant of natural variation in leaf cadmium in Arabidopsis thaliana [PDF]

, 2012
Peer reviewedPublisher ...
Baxter, Ivan, Chao, Dai-Yin, Danku, John, Huang, Yu S, Lahner, Brett, Nordborg, Magnus, Salt, David E, Silva, Adriano, Yakubova, Elena   +8 more
core   +6 more sources

Mapping spot blotch resistance genes in four barley populations [PDF]

, 2010
Bipolaris sorokiniana (teleomorph: Cochliobolus sativus) is the fungal pathogen responsible for spot blotch in barley (Hordeum vulgare L.) and occurs worldwide in warmer, humid growing conditions.
A Lehmensiek, A Lehmensiek, AK Joshi, Anke Lehmensiek, BJ Steffenson, BJ Steffenson, Emma Mace, GM Murray, Greg J. Platz, H Bilgic, H Bilgic, H Ghazvini, H Ghazvini, H Os Van, J Kumar, Jerome Franckowiak, Jessica Bovill, KF Manly, LC Emebiri, Mark W. Sutherland, MIE Arabi, MVG Müller, P Wenzl, P Wenzl, RE Voorrips, S Zhong, SI Meldrum, T Drader, T Kuldeep, Terry Usher, TG Fetch, TG Fetch, TS Grewal   +32 more
core   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

Phytopathology Research, 2020
Plant virus-induced diseases cause significant losses to agricultural crop production worldwide. Reverse genetics systems of plant viruses allow gene manipulation on viral genomes, which greatly facilitates studies of viral pathogenesis and interactions ...
Ying Zang, Xiao-Dong Fang, Ji-Hui Qiao, Qiang Gao, Xian-Bing Wang   +4 more
doaj   +1 more source

Engineering recombinant orsay virus directly in the metazoan host Caenorhabditis elegans [PDF]

, 2014
The recent identification of Orsay virus, the first virus that is capable of naturally infecting Caenorhabditis elegans, provides a unique opportunity to explore host-virus interaction studies in this invaluable model organism.
Franz, Carl J, Jiang, Hongbing, Wang, David   +2 more
core   +2 more sources

Reverse genetics for mammalian reovirus [PDF]

Methods, 2011
Mammalian orthoreoviruses (reoviruses) are highly tractable models for studies of viral replication and pathogenesis. The versatility of reovirus as an experimental model has been enhanced by development of a plasmid-based reverse genetics system. Infectious reovirus can be recovered from cells transfected with plasmids encoding cDNAs of each reovirus ...
Karl W, Boehme, Miné, Ikizler, Takeshi, Kobayashi, Terence S, Dermody   +3 more
openaire   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Characterization of a human H3N8 influenza virusResearch in context

EBioMedicine
Summary: Background: In 2022 and 2023, novel reassortant H3N8 influenza viruses infected three people, marking the first human infections with viruses of this subtype.
Chunyang Gu, Shufang Fan, Randall Dahn, Lavanya Babujee, Shiho Chiba, Lizheng Guan, Tadashi Maemura, David Pattinson, Gabriele Neumann, Yoshihiro Kawaoka   +9 more
doaj   +1 more source