Results 31 to 40 of about 1,866 (214)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease.

PLoS ONE
The encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen, Matthew J Abbott, Alyssa B Evans   +2 more
doaj   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

PLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu, Ying Shi, Dawei Yan, Xuesong Li, Pixi Yan, Xuyuan Gao, Yuee Zhang, Lei Yu, Chaochao Ren, Guoxin Li, Liping Yan, Qiaoyang Teng, Zejun Li   +12 more
doaj   +1 more source

Modern Approaches to Molecular Genetics of Viruses in the Study of the Members of the Family Coronaviridae

Вестник войск РХБ защиты, 2021
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation

Virology Journal
Influenza is a highly contagious acute viral illness that affects the respiratory system, posing a significant global public health concern. Influenza B virus (IBV) causes annual seasonal epidemics.
Ghayyas Ud Din, Chunchen Wu, Zahra Tariq, Kinza Hasham, Muhammad Nabeel Amjad, Bei Shen, Lihuan Yue, Muhammad Asif Raza, Muhammad Awais Ashraf, Lingdie Chen, Yihong Hu   +10 more
doaj   +1 more source

Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons

Bio-Protocol
Human astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali, David Noyvert, Valeria Lulla   +2 more
doaj   +1 more source

Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

Phytopathology Research, 2020
Plant virus-induced diseases cause significant losses to agricultural crop production worldwide. Reverse genetics systems of plant viruses allow gene manipulation on viral genomes, which greatly facilitates studies of viral pathogenesis and interactions ...
Ying Zang, Xiao-Dong Fang, Ji-Hui Qiao, Qiang Gao, Xian-Bing Wang   +4 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Characterization of a human H3N8 influenza virusResearch in context

EBioMedicine
Summary: Background: In 2022 and 2023, novel reassortant H3N8 influenza viruses infected three people, marking the first human infections with viruses of this subtype.
Chunyang Gu, Shufang Fan, Randall Dahn, Lavanya Babujee, Shiho Chiba, Lizheng Guan, Tadashi Maemura, David Pattinson, Gabriele Neumann, Yoshihiro Kawaoka   +9 more
doaj   +1 more source