Results 31 to 40 of about 1,319,277 (281)

Reverse Genetics of RNA Viruses: Methods and Protocols

, 2023
Reverse Genetics Systems for Filoviruses -- Reverse Genetics Systems for the de novo Rescue of Diverse Members of Paramyxoviridae -- Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes -- Use of Reverse Genetics for the Generation

core   +1 more source

Cytokines, reverse genetics and anti-cytokine therapy

Бюллетень сибирской медицины, 2019
Cytokines comprise the molecular language of communication between the cells, which is needed to maintain the homeostatic functions of the body (including the immune system) and mediate various diseases.
M. S. Drutskaya, E. O. Gubernatorova, E. A. Gorshkova, K.-S. N. Athertkhany, M. A. Nosenko, V. S. Gogoleva, O. A. Namakanova, R. V. Zvartsev, A. A. Kruglov, S. A. Nedospasov   +9 more
doaj   +1 more source

Reverse Genetics for Double-Stranded RNA Viruses

Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 2009
Resumen en inglès, ver archivo ...
H. Attoui, F. Mohd Jaafar, S. Maan, P. P.C. Mertens   +3 more
doaj   +1 more source

Advantages, disadvantages and research progress on strategies for screening and identifying functional genes [PDF]

Jichu yixue yu linchuang, 2022
The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and ...
LIU Xu, CHANG De, WANG Jun-feng
doaj   +1 more source

Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes

Viruses, 2020
Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively.
Hiromichi Matsugo, Tomoya Kobayashi-Kitamura, Haruhiko Kamiki, Hiroho Ishida, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto   +6 more
doaj   +1 more source

Modern approaches to production of safe and effective genetically modified rabies vaccines for animals

Ветеринария сегодня, 2023
Rabies is a dangerous zoonoticdisease that affects the central nervous system, causes encephalomyelitis and paralyses and Is almost invariably fatal.
M. I. Doronin, A. Mazloum, D. V. Mikhalishin, M. N. Mitrofanova, A. Yu. Sukharkov, V. V. Kiseleva, A. V. Sprygin   +6 more
doaj   +1 more source

Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus

Viruses, 2020
Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ...
Anja Dalmann, Kerstin Wernike, Eric J. Snijder, Nadia Oreshkova, Ilona Reimann, Martin Beer   +5 more
doaj   +1 more source

Rapid reconstruction of porcine reproductive and respiratory syndrome virus using synthetic DNA fragments

Computational and Structural Biotechnology Journal, 2021
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry.
Julien Mélade, Géraldine Piorkowski, Hawa Sophia Bouzidi, Alain Medawar, Claudine Raffy, Xavier de Lamballerie, Antoine Nougairède   +6 more
doaj   +1 more source

Rapid Reverse Genetics Systems for Rhabdoviruses: From Forward to Reverse and Back Again

, 2017
Methods to recover recombinant negative strand RNA viruses (rNSVs) from cloned cDNAs have been significantly improved in more than two decades of NSV reverse genetics .
Nolden, Tobias, Tobias Nolden, Stefan Finke, Finke, Stefan   +3 more
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One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology

Frontiers in Microbiology, 2022
Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally.
Yanyang Zhou, Chenxi Li, Chenxi Li, Chenxi Li, Cicheng Ren, Jingbo Hu, Changxu Song, Xinjie Wang, Yanhua Li, Yanhua Li, Yanhua Li   +10 more
doaj   +1 more source