Results 31 to 40 of about 668,266 (290)

Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening [PDF]

open access: yes, 2012
Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants
Fan, Zaifeng   +15 more
core   +3 more sources

Evaluation of Stem-Loop Reverse Transcription and Poly-A Tail Extension in MicroRNA Analysis of Body Fluids [PDF]

open access: yes, 2014
MicroRNA has been demonstrated to be a viable tool for body fluid identification purposes in forensic casework. Stem-loop reverse transcription (slRT) is regularly used for cDNA synthesis from mature miRNA, along with poly-A tail extension.
Dunnett, Hannah   +2 more
core   +1 more source

Cytokines, reverse genetics and anti-cytokine therapy

open access: yesБюллетень сибирской медицины, 2019
Cytokines comprise the molecular language of communication between the cells, which is needed to maintain the homeostatic functions of the body (including the immune system) and mediate various diseases.
M. S. Drutskaya   +9 more
doaj   +1 more source

Reverse Genetics for Double-Stranded RNA Viruses

open access: yesRevue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 2009
Resumen en inglès, ver archivo ...
H. Attoui   +3 more
doaj   +1 more source

Advantages, disadvantages and research progress on strategies for screening and identifying functional genes [PDF]

open access: yesJichu yixue yu linchuang, 2022
The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and ...
LIU Xu, CHANG De, WANG Jun-feng
doaj   +1 more source

Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR. [PDF]

open access: yes, 2018
Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here,
Akbari, Omar S   +2 more
core   +2 more sources

Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes

open access: yesViruses, 2020
Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively.
Hiromichi Matsugo   +6 more
doaj   +1 more source

Modern approaches to production of safe and effective genetically modified rabies vaccines for animals

open access: yesВетеринария сегодня, 2023
Rabies is a dangerous zoonoticdisease that affects the central nervous system, causes encephalomyelitis and paralyses and Is almost invariably fatal.
M. I. Doronin   +6 more
doaj   +1 more source

Occurrence of LINE, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (Ipomoea batatas L.) [PDF]

open access: yes, 2011
Retrotransposons are a class of transposable elements that represent a major fraction of the repetitive DNA of most eukaryotes. Their abundance stems from their expansive replication strategies.
Dieters, Mark   +3 more
core   +2 more sources

Rapid reconstruction of porcine reproductive and respiratory syndrome virus using synthetic DNA fragments

open access: yesComputational and Structural Biotechnology Journal, 2021
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry.
Julien Mélade   +6 more
doaj   +1 more source

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