Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons [PDF]
Bio-ProtocolHuman astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali +2 more
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Developments in Negative-Strand RNA Virus Reverse Genetics [PDF]
MicroorganismsMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang +6 more
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Efficient Plasmid-Based Rescue of T7 RNA Polymerase-Driven Calicivirus Reverse Genetics Systems in Mammalian Cells Using Vaccinia Virus RNA-Capping Enzymes [PDF]
VirusesThe caliciviruses include important human and animal pathogens such as norovirus, sapovirus and feline calicivirus. Viral reverse genetics is performed to understand the fundamental biology of these viruses, as well as a potential route to generate live ...
Frazer J. T. Buchanan +7 more
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Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine [PDF]
Journal of VirologyControl of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection ...
Tendai A. M. Mlingo +2 more
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Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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A Reverse Genetics System for Cypovirus Based on a Bacmid Expressing T7 RNA Polymerase
Viruses, 2019 Dendrolimus punctatus cypovirus (DpCPV), belonging to the genus Cypovirus within the family Reoviridae, is considered the most destructive pest of pine forests worldwide. DpCPV has a genome consisting of 10 linear double-stranded RNA segments.
Gaobo Zhang +7 more
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Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis
Viruses, 2021 Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto +3 more
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Reverse genetics in eukaryotes [PDF]
Biology of the Cell, 2010 Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations.
Hardy, Serge +3 more
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Reverse Genetics System for Rabbit vesivirus
Frontiers in Microbiology, 2020 Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
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Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
Инфекция и иммунитет, 2023 Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of ...
N. V. Larionova +4 more
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