Results 11 to 20 of about 1,319,277 (281)
Reverse genetics in ecological research.
PLoS ONE, 2008 By precisely manipulating the expression of individual genetic elements thought to be important for ecological performance, reverse genetics has the potential to revolutionize plant ecology.
Jens Schwachtje +2 more
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Reverse Genetics of Filoviruses
, 2017 Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and host ...
Brandt, Janine +9 more
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A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease. [PDF]
PLoS ONEThe encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen +2 more
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Marburg Virus Reverse Genetics Systems [PDF]
Viruses, 2016 The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses.
Kristina Maria Schmidt, Elke Mühlberger
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Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons [PDF]
Bio-ProtocolHuman astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali +2 more
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Developments in Negative-Strand RNA Virus Reverse Genetics
MicroorganismsMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang +6 more
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Reverse Genetics Systems for Filoviruses
, 2023 Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the ...
Bodmer, Bianca S., Hoenen, Thomas
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Reverse Genetics Systems for Filoviruses
, 2017 Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such ...
Thomas Hoenen +3 more
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Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine [PDF]
Journal of VirologyControl of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection ...
Tendai A. M. Mlingo +2 more
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Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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