RNA Virus Reverse Genetics and Vaccine Design
Viruses, 2014 RNA viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. The development of reverse genetics systems for manipulation and study of RNA virus genomes has provided platforms for designing and optimizing ...
Christopher C. Stobart, Martin L. Moore
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Developments in Negative-Strand RNA Virus Reverse Genetics [PDF]
MicroorganismsMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang +6 more
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Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation [PDF]
Virology JournalInfluenza is a highly contagious acute viral illness that affects the respiratory system, posing a significant global public health concern. Influenza B virus (IBV) causes annual seasonal epidemics.
Ghayyas Ud Din +10 more
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Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine [PDF]
Journal of VirologyControl of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection ...
Tendai A. M. Mlingo +2 more
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Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. [PDF]
PLoS ONEAvian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables ...
Katharina Bilotti +13 more
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Reverse genetics in virology: A double edged sword
Biosafety and Health, 2022 Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.
Hongyu Chen, Hongqi Liu, Xiaozhong Peng
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Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis
Viruses, 2021 Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto +3 more
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Reverse Genetics System for Rabbit vesivirus
Frontiers in Microbiology, 2020 Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
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Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
Инфекция и иммунитет, 2023 Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of ...
N. V. Larionova +4 more
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