Results 121 to 130 of about 52,296 (305)
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Behavioral SciencesWhile sadness and anger are distinct emotional states, the cognitive traits that differentiate people prone to one versus the other are not well understood. This research tested whether the cognitive signatures of state emotions extend to the trait level.
Seunghee Han
doaj +1 more source
The generality of learned helplessness theory: effect of electroconvulsive shock [PDF]
, 1977 While the learned helplessness effect has been reliably found in dogs and other species (e.g., cats, mice, fish, and humans), it has been somewhat difficult to obtain in rats.
Brett, Claude William
core
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
The Hopelessness and Helplessness Scale: Validity and measurement invariance
Journal of Happiness and HealthThis study set out to investigate the psychometric properties of the Hopelessness and Helplessness Scale (HHS), which was designed to measure an individual’s levels of hopelessness and helplessness.
Erdinç Duru, Murat Balkis, Sibel Duru
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Şırnak Üniversitesi İlahiyat Fakültesi DergisiÜniversite öğrencilerinde dini motivasyon, iyimserlik ve çaresizlik değişkenleri arasındaki ilişkileri inceleyen bu araştırmanın temel amacı dini motivasyonun öğrencilerde çaresizlik gibi olumsuz psikolojik durumları azaltmadaki rolünü ortaya koymak ve ...
Yunus Emre Temiz
doaj +1 more source