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Hemagglutination Inhibition Assay

2020
The hemagglutination inhibition (HI) assay for influenza A virus has been used since the 1940s. The assay may be utilized to detect or quantify antibodies to influenza A viruses and can be used to characterize differences in antigenic reactivity between influenza isolates.
Erica, Spackman, Ioannis, Sitaras
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Hemagglutination and Hemagglutination-Inhibition Studies with Echo Viruses

The Journal of Immunology, 1962
Summary A study of several factors which may affect hemagglutinin (HA) production revealed that a dilute inoculum combined with a relatively late harvest time favored maximum HA titers of ECHO 6 viruses grown in monkey kidney tissue culture.
R H, BUSSELL, D T, KARZON, F T, HALL
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Hemagglutination and Hemagglutination - Inhibition with Killed Mycoplasma Antigen

Avian Diseases, 1965
Hemagglutination (HA) and hemagglutination-inhibition (HI) with Mycoplasma gallisepticum (MG) were first demonstrated by van Herick and Eaton (5). Little use was made of these findings until Jungherr et al. (2) reintroduced the procedure, in 1953. Thereafter, numerous investigators used the HI test for detecting antibodies for M. gcllisepticum.
M, Baharsefat, H E, Adler
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Hemagglutination Test for Toxoplasmosis

Science, 1957
The importance of adequate serological tests for toxoplasmosis is accentuated by the tremendous gains in knowledge concerning this infection, which stemmed from the description of the in vitro dye test by Sabin and Feldman in 1948. In the less than 8 years since this discovery, the serology of toxoplasmosis has been characterized and forms of the ...
L, JACOBS, M N, LUNDE
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Hemagglutination (Inhibition) Assay

2014
The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension.
Kotone, Sano, Haruko, Ogawa
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Passive Hemagglutination

The Journal of Immunology, 1970
Abstract Aggregates of mildly denatured bovine serum albumin (BSA) present antigenic determinants which are not found in native or denatured but unaggregated BSA. Antibody against these determinants appears early and often transiently in rabbits immunized with denatured or native BSA emulsified in Freund's incomplete adjuvant.
Gerald Wolberg   +2 more
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Hemagglutination properties of Enterococcus

Current Microbiology, 1995
In total, 86 enterococcal strains including representatives of most of the described species were tested for the ability to agglutinate human, sheep, and rabbit erythrocytes. Five strains did not react with any of the erythrocytes tested, and 81 (94.2%) strains agglutinated only rabbit erythrocytes. The hemagglutination titers ranged from 2 to 64. Loss
M da G, Carvalho, L M, Teixeira
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Cryopreserved erythrocytes in clinical laboratory hemagglutination and hemagglutination inhibition tests

Journal of Clinical Microbiology, 1985
Various concentrations of pig, guinea pig, chicken, and turkey erythrocytes (RBCs) were cryopreserved and compared with fresh RBCs in hemagglutination and hemagglutination inhibition tests. Hemagglutination and hemagglutination inhibition titers obtained with cryopreserved or fresh RBCs from the same donor differed by not more than one twofold dilution.
R K, Maes, M M, Hayes, J P, Newman
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Blue dextran-mediated hemagglutination

Journal of Immunological Methods, 1982
Blue dextran at low concentrations (0.1-1 ng/ml) agglutinated human, mouse, rabbit and rat erythrocytes. This agglutination was inhibited by 10% calf serum, 0.5 mg/ml bovine albumin and 0.2 M sodium thiocyanate, and less effectively by 1.5 M potassium chloride, but not by 30-50 mM magnesium sulfate.
K, Imanishi, I, Suzuki
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Techniques for Hemagglutination and Hemagglutination-Inhibition with Arthropod-Borne Viruses

The American Journal of Tropical Medicine and Hygiene, 1958
Preparation of Antigens 561; Preparation of Sera 567; Preparation of Erythrocytes 568; Chemical Reagents 569; Technique of HA and HI Tests 570; HA Titrations 571; HI Tests 572; References 573 During the past few years, the methods for conducting hemagglutination (HA) and hemagglutination-inhibition (HI) tests with arthropod-borne (arbor) viruses have ...
D H, CLARKE, J, CASALS
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