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Hemagglutination Assay For Determination Of Human Factor VIII:C [PDF]
New methods for the determination of human factor VIII clotting protein (F. VIII:C) have been developed utilizing hemagglutination technique without using any other coagulation proteins in the assay. A 50% ammonium sulphate precipitate of the plasma from a hemophilic patient containing high titer of F.
E P Kang +3 more
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1992
Publisher Summary This chapter discusses hemagglutination assay. Hemagglutination is the aggregation of red blood cells in the presence of hemagglutinating virus particles. This phenomenon is because of the presence of outer surface proteins on the hemagglutinating virus that recognize and attach to cellular surface receptors expressed by RBCs.
FLORENCE G. BURLESON +2 more
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Publisher Summary This chapter discusses hemagglutination assay. Hemagglutination is the aggregation of red blood cells in the presence of hemagglutinating virus particles. This phenomenon is because of the presence of outer surface proteins on the hemagglutinating virus that recognize and attach to cellular surface receptors expressed by RBCs.
FLORENCE G. BURLESON +2 more
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Hemagglutination Assay for the Avian Influenza Virus [PDF]
The hemagglutination (HA) assay is a tool used to screen cell culture or amnioallantoic fluid harvested from embryonating chicken eggs for hemagglutinating agents, such as type A influenza. The HA assay is not an identification assay, as other agents also have hemagglutinating properties.
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Hemagglutination inhibition assays
Seminars in Avian and Exotic Pet Medicine, 2002Hemagglutination is a useful characteristic of many viruses because it can provide a simple and efficient method of detecting antihemagglutinating activity in serum from exposed animals. This is called hemagglutination inhibition (HI), and HI assays are used extensively to detect and titrate antibodies to many avian diseases including Newcastle disease
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Potato lectin activity assay based on hemagglutination
Journal of the Science of Food and Agriculture, 1992AbstractA quantitative method for the determination of potato lectin activity has been developed. In the method, lectin is incubated with an excess of red blood cells (RBC) in phosphate buffered saline at 25 °C. Maximum agglutination is reached within 60 min. Agglutinated RBC are then separated from nonagglutinated RBC by filtration.
T. W. Willis, C. P. Patel
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Development of a Hemagglutination Inhibition Assay for Duck Tembusu Virus
Avian Diseases, 2019The HB strain of duck Tembusu virus (DTMUV) propagated in the brains of newborn mice was used to prepare antigens for use in the hemagglutination inhibition (HI) test. Results showed that such prepared antigens are highly specific to the serum samples derived from DTMUV-infected animals.
Xiuqing Wang +11 more
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Passive Hemagglutination Assays for the Detection of Antibodies to Herpes Viruses
Microbiology and Immunology, 1993AbstractA simple and effective method for the detection of antibodies to herpes simplex virus (HSV), human cytomegalovirus (HCMV) and varicella‐zoster virus (VZV), has been established using the passive hemagglutination assay (PHA) in combination with viral specific glycoproteins.
Yoichi Minamishima, Yoichiro Kino
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Improved analysis of hemagglutination assays for quantitation of lectin activity
Analytical Biochemistry, 1980Abstract A method to quantitate lectin activity based on hemagglutination assay in microtiter plates is described. In addition to the normal method of visual titer evaluation an electronic particle counter is used for counting of nonagglutinated cells in the microtiter wells; this allows a rapid, quantitative determination of the amount of lectin ...
Harald Köhle, Heinrich Kauss
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Hemagglutination Assay for Antigen and Antibody Associated with Viral Hepatitis
Science, 1970Hemagglutination assays are described for measuring hepatitis-associated Australia antigen and antibody. Red cells coated with isolated antigen, with chromic chloride as a coupling agent, are used for detection of antibodies. Detection of the antigen in serums depends on inhibition of hemagglutination.
Girish N. Vyas, N. R. Shulman
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Hemagglutination Assay for Influenza Virus
2014The hemagglutination assay (HA) is a tool used to screen cell culture isolates or amnioallantoic fluid harvested from embryonated chicken eggs for hemagglutinating agents, such as type A influenza. The HA assay is not an identification assay, as other agents also have hemagglutinating properties. Live and inactivated viruses are detected by the HA test.
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