Assay Harmonization and Use of Biological Standards To Improve the Reproducibility of the Hemagglutination Inhibition Assay: a FLUCOP Collaborative Study [PDF]
mSphere, 2021 The hemagglutination inhibition (HAI) assay is an established technique for assessing influenza immunity, through measurement of antihemagglutinin antibodies.
Joanna Waldock +12 more
doaj +8 more sources
Interlaboratory Reproducibility of Standardized Hemagglutination Inhibition Assays
mSphere, 2022 The hemagglutination inhibition (HI) assay is a prominent and commonly accepted method used to determine quantitative antibody titers for influenza virus.
David C. Bibby +6 more
doaj +5 more sources
A Novel Application of Virus Like Particles in the Hemagglutination Inhibition Assay [PDF]
International Journal of Molecular SciencesThe hemagglutination inhibition (HI) assay is a traditional laboratory procedure for detection and quantitation of serum antibodies of hemagglutinating viruses containing the hemagglutinin (HA) gene. The current study aimed to investigate the novel use of virus like particles (VLP) as an antigen for the HI assay.
Abdelsatar Arafa +2 more
exaly +4 more sources
A Competitive Hemagglutination Inhibition Assay for Dissecting Functional Antibody Activity against Influenza Virus [PDF]
Journal of Virology, 2021 The hemagglutination inhibition (HAI) assay is a well-established and reproducible method that quantifies functional antibody activity against influenza viruses and, in particular, the capability of an antibody formulation to inhibit the binding of hemagglutinin (HA) to sialic acid.
Greg Kirchenbaum +2 more
exaly +4 more sources
Development and Efficacy Evaluation of a Novel Nanoparticle-Based Hemagglutination Inhibition Assay for Serological Studies of Porcine Epidemic Diarrhea Virus [PDF]
Veterinary SciencesPorcine epidemic diarrhea virus (PEDV) is a major pathogen that causes serious economic losses to the swine industry. To aid PEDV clinical diagnosis and vaccine development, sensitive and precise serological methods are demanded for rapid detection of ...
Fengyan Liang +7 more
doaj +4 more sources
Diagnostic performance of the canine Influenza A Virus subtype H3N8 hemagglutination inhibition assay [PDF]
Journal of Veterinary Diagnostic Investigation, 2012 Canine Influenza A virus subtype H3N8 (H3N8 CIV) was recognized in 2004 as a novel respiratory pathogen for dogs. To date, infections have been diagnosed in thousands of dogs in 38 U.S. states. Diagnostic techniques such as reverse transcription polymerase chain reaction (RT-PCR) and virus isolation may yield false-negative results if samples are ...
Edward Dubovi
exaly +5 more sources
Automated Imaging and Analysis of the Hemagglutination Inhibition Assay [PDF]
SLAS Technology, 2016 The hemagglutination inhibition (HAI) assay quantifies the level of strain-specific influenza virus antibody present in serum and is the standard by which influenza vaccine immunogenicity is measured. The HAI assay endpoint requires real-time monitoring of rapidly evolving red blood cell (RBC) patterns for signs of agglutination at a rate of ...
William Warren
exaly +3 more sources
Hemagglutination Inhibition Assay for Gentamicin [PDF]
Antimicrobial Agents and Chemotherapy, 1977 A rapid and simple hemagglutination inhibition test for measuring gentamicin concentrations in serum has been developed. Correlation coefficients for this assay with the radioimmunoassay and microbiological assay were r = 710 and r = 0.864, respectively.
W A, Mahon, R I, Feldman, G H, Scherr
exaly +3 more sources
Hemagglutination Inhibition (HI) Assay of Influenza Viruses with Monoclonal Antibodies
Bio-Protocol, 2016 Heamagglutination is inhibited when antibodies are present because antibodies to the influenza virus will prevent attachment of the virus to red blood cells. The highest dilution of antibody that prevents hemagglutination is called the HI titer.
Ying Wu +24 more
doaj +2 more sources
Standardization of Hemagglutination Inhibition Assay for Influenza Serology Allows for High Reproducibility between Laboratories. [PDF]
Clin Vaccine Immunol, 2016 ABSTRACT Standardization of the hemagglutination inhibition (HAI) assay for influenza serology is challenging. Poor reproducibility of HAI results from one laboratory to another is widely cited, limiting comparisons between candidate vaccines in different clinical trials and posing challenges for licensing authorities.
Zacour M +10 more
europepmc +3 more sources