Results 161 to 170 of about 2,865 (201)

Purification and stability of epizootic hemorrhagic disease virus

Journal of Virological Methods, 1982
Purification of epizootic hemorrhagic disease virus (EHDV) from sonicated cell culture supernatant was effected using polyethylene glycol (PEG) precipitation and two CsCl density gradient centrifugations. During purification procedures the pH was maintained at values close to pH 8.0 as pH stability for EHDV was maximal between pH 7.2 and pH 9.0. During
R, Lewanczuk, T, Yamamoto
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Clinical disease associated with epizootic hemorrhagic disease virus in cattle in Illinois

Journal of the American Veterinary Medical Association, 2015
Abstract Objective—To describe the clinical manifestation of epizootic hemorrhagic disease virus (EHDV) in cattle during an outbreak in northwestern Illinois in the fall of 2013. Design—Outbreak survey. Sample—18 EHDV-affected cattle herds. Procedures—Herds with confirmed EHDV-infected cattle were selected for the survey on the basis of having a ...
Edgar F, Garrett, Eleonora, Po, Elena R, Bichi, Suzette K, Hexum, Robert, Melcher, Andrew M, Hubner   +5 more
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Experimental infection of calves with epizootic hemorrhagic disease virus

American Journal of Veterinary Research, 1999
Abstract Objective To determine whether experimental inoculation with a field strain of epizootic hemorrhagic disease virus serotype-2 (EHDV-2) suspected of causing clinical disease in naturally infected cattle would cause clinical disease in calves. Animals 8 calves.
M J, Abdy, E E, Howerth, D E, Stallknecht   +2 more
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The association of epizootic hemorrhagic disease virus with the cytoskeleton

Proceedings, annual meeting, Electron Microscopy Society of America, 1990
The cells of eukaryotes are characterized by a filamentous network referred to as the cytoskeleton. It is believed that most animal viruses use the cytoplasmic or nuclear skeletal matrix during at least part of their replication cycle.Transmission electron microscopic studies of thin sections of cells infected with epizootic hemorrhagic disease virus ...
R.A. Nunamaker, J.G. Wigington, C.E. Nunamaker   +2 more
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Epizootic Hemorrhagic Disease Virus in Montana: Isolation and Serologic Survey

American Journal of Veterinary Research, 1981
SUMMARY Epizootic hemorrhagic disease virus (ehdv) was isolated in Vero cell culture from the spleen and whole blood of a white-tailed deer (Odocoileus virginianus). A 10% spleen suspension caused acute hemorrhagic disease (hd) when inoculated into an experimental white-tailed deer and resulted in the recovery of ehdv from the blood of the experimental
T J, Feldner, M H, Smith
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Serological evidence of epizootic hemorrhagic disease and serotypes of epizootic hemorrhagic disease virus in Pakistan

Acta Tropica
This study investigated seroprevalence and serotypes of Epizootic hemorrhagic disease virus (EHDV) in ruminants in North-Western Pakistan. A total of 616 serum samples were tested for the presence of antibodies against EHDV and neutralizing antibodies against 7 EHDV serotypes (EHDV-1, 2, 4, 5, 6, 7 and 8) using competitive ELISA and VNT, respectively ...
Muhammad Ishaq, Syed M. Jamal, Liana Teodori, Alessandra Leone, Barbara Bonfini, Massimo Spedicato, Giovanni Savini   +6 more
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No evidence for involvement of sheep in the epidemiology of cattle virulent epizootic hemorrhagic disease virus

Veterinary Microbiology, 2011
Epizootic hemorrhagic disease virus (EHDV) is an Orbivirus. While not previously considered as an important disease in cattle, several EHDV serotypes (EHDV-6 and 7) have recently been implicated in disease outbreaks. The involvement of sheep in the epidemiology of EHDV is still not understood.
H Yadin, Carrie Batten, Eyal Klement
exaly   +3 more sources

Epizootic hemorrhagic disease virus of deer: an electron microscopic study

Canadian Journal of Microbiology, 1970
A mouse brain adapted strain of epizootic hemorrhagic disease (EHD) virus of deer was studied by ultrathin section and negative contrast electron microscopy. In sections of infected BHK-21 cells and neurons of newborn mouse brains, aggregates of virus particles, some appearing to be lacking their outer coats, were observed within membrane-bound ...
K S, Tsai, L, Karstad
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Plaque Neutralization of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus in BHK21 Cells

American Journal of Veterinary Research, 1976
SUMMARY Plaque assay and plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus were studied in baby hamster kidney (bhk21) cells grown under an overlay containing gum tragacanth. Tests were done in plastic panels, each with 24 wells, and variables were established for achieving reproducible results.
M M, Jochim, S C, Jones
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EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS

Journal of Wildlife Diseases, 1973
Two elk (Cervus canadensis) were inoculated intravenously with epizootic hemorrhagic disease virus. No overt signs of disease were observed in the elk although one animal had a slight febrile response. A cell associated viremia was detected in both elk, which persisted in one animal for 30 days post-inoculation.
G L, Hoff, D O, Trainer
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