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A human iPSC-derived kidney-liver organ-on-a-chip platform for modeling inter-organ crosstalk.

open access: yesLab Chip
de Brouwer L   +10 more
europepmc   +1 more source
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Benchmarking HEp-2 Cells Classification Methods

IEEE Transactions on Medical Imaging, 2013
In this paper, we report on the first edition of the HEp-2 Cells Classification contest, held at the 2012 edition of the International Conference on Pattern Recognition, and focused on indirect immunofluorescence (IIF) image analysis. The IIF methodology is used to detect autoimmune diseases by searching for antibodies in the patient serum but ...
FOGGIA, PASQUALE   +3 more
openaire   +4 more sources

Adherence of Helicobacter pylori to HEp-2 cells

Journal of Infection, 1992
The adherence of 25 strains of Helicobacter pylori was evaluated in HEp-2 cells. These bacterial isolates, obtained from Chilean patients with gastric disorders, were also tested for haemagglutination of human red blood cells. Adherence of HEp-2 cells was expressed as a common property of all strains, irrespective of whether the cultures were grown on ...
Figueroa, Guillermo   +3 more
openaire   +3 more sources

Effect of the bioflavonoid morin on HEp-2 cells

Bulletin of Environmental Contamination and Toxicology, 1994
Bioflavonoids are plant compounds which aid in disease resistance (MariniBettolo and Monache 1977) and attract pollinating insects (Thompson et al. 1972). Flavonoids have been shown to affect biological systems variably, having antibacterial, antiviral (Miski et al. 1983), antifungal (Donnelly 1977), mutagenic (Brown 1980), antineoplastic and/or enzyme
A L, Pond, J M, Zamora, M R, Wells
openaire   +2 more sources

Mitotic cells recognition in HEp-2 images

Pattern Recognition Letters, 2014
Abstract Indirect immunofluorescence is the recommended method to detect autoantibodies in patient serum, which could cause connective tissue diseases. One step of the diagnostic procedure needs to distinguish between mitotic and interphase cells in HEp-2.
Giulio Iannello   +3 more
openaire   +2 more sources

Escherichia coli adherence to HEp-2 cells with prefixed cells

Journal of Clinical Microbiology, 1995
We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live
H M, Zepeda-Lopez, G M, Gonzalez-Lugo
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Adherence and internalization of Helicobacter pylori by HEp-2 cells

Gastroenterology, 1992
Helicobacter pylori colonizes the mucous layer of the stomach and the surface of gastric mucous cells. Although H. pylori is not generally thought of as invasive, it has been observed in the lamina propria and within vacuoles in the cytoplasm of epithelial cells. The authors report that isolates of H.
D G, Evans, D J, Evans, D Y, Graham
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Automatic Analysis of Immunofluorescence Patterns of HEp‐2 Cells

Annals of the New York Academy of Sciences, 2007
Abstract:  The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp‐2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available.
Rico, Hiemann   +7 more
openaire   +2 more sources

Ultrastructure of Hep-2 Cells

1985
Abstract Hep-2 cells are commonly used as substrate in immunofluorescence tests. At the ultrastructural level, the nucleus of HEp-2 cell is characterized by a small amount of dense chromatin and, in the interchromatinic areas, by large nucleoli and by several types of nuclear bodies.
M. THIRY, R. HUMBEL, G. GOESSENS
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A classification-based approach to segment HEp-2 cells

2012 25th IEEE International Symposium on Computer-Based Medical Systems (CBMS), 2012
In this paper we propose a new method for cells segmentation in HEp-2 images addressing and overcoming the main limitations of the existing approaches. The proposed method adopts image reconstruction for a preliminary image segmentation and, then, it employs a sort of classifier-controlled dilation for better determining the structure of the cells ...
PERCANNELLA, Gennaro   +2 more
openaire   +3 more sources

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