Results 121 to 130 of about 816 (153)

Regulation of Nitrogen Fixation in Herbaspirillum seropedicae

1998
Herbaspirillum seropedicae is an aerobic, plant-associated, nitrogen-fixing bacterium of the β-proteobacteria group. Nitrogen fixation in this organism is repressed by ammonium ions or oxygen, and nitrogenase switch-off by ammonium ions is partial and does not involve ADP-ribosylation (Klasssen et al., unpublished). The nif genes of H.
F. O. Pedrosa, E. M. Souza, E. M. Benelli, I. M. P. Machado, M. B. R. Steffens, G. Klassen, H. B. Machado, L. U. Rigo, R. Wassem, R. A. Monteiro, F. G. M. Rego, L. S. Chubats, M. Buck, M. Moore, A. Harper, M. Drummond, M. G. Yates   +16 more
openaire   +1 more source

Role of conserved cysteine residues in Herbaspirillum seropedicae NifA activity

Research in Microbiology, 2009
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium that associates with economically important crops. NifA protein, the transcriptional activator of nif genes in H. seropedicae, binds to nif promoters and, together with RNA polymerase-sigma(54) holoenzyme, catalyzes the formation of open complexes to allow transcription initiation.
Marco A S, Oliveira, Valter A, Baura, Bruno, Aquino, Luciano F, Huergo, Marco A S, Kadowaki, Leda S, Chubatsu, Emanuel M, Souza, Ray, Dixon, Fábio O, Pedrosa, Roseli, Wassem, Rose A, Monteiro   +10 more
openaire   +2 more sources

In vitro characterization of the NAD+ synthetase NadE1 from Herbaspirillum seropedicae

Archives of Microbiology, 2016
Nicotinamide adenine dinucleotide synthetase enzyme (NadE) catalyzes the amination of nicotinic acid adenine dinucleotide (NaAD) to form NAD(+). This reaction represents the last step in the majority of the NAD(+) biosynthetic routes described to date. NadE enzymes typically use either glutamine or ammonium as amine nitrogen donor, and the reaction is ...
Kerly, Laskoski, Adrian R S, Santos, Ana C, Bonatto, Fábio O, Pedrosa, Emanuel M, Souza, Luciano F, Huergo   +5 more
openaire   +2 more sources

Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae

Journal of Microbiological Methods, 2016
The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values.
Daniella Duarte Villarinho, Pessoa, Marcia Soares, Vidal, José Ivo, Baldani, Jean Luiz, Simoes-Araujo   +3 more
openaire   +2 more sources

Indentification of Acetobacter diazotrophicus, Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans using biochemical and genetic criteria

Plant and Soil, 1995
Nitrogen-fixing Acetobacter diazotrophicus, Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans colonize sugar cane, and are thought to be capable of supplying high levels of fixed nitrogen to this plant. Eight A. diazotrophicus, two H. seropedicae and four H.
A. Ureta, B. Alvarez, A. Ram�n, M. A. Vera, G. Martinez-Drets   +4 more
openaire   +1 more source

A Report on the Genome of Herbaspirillum seropedicae Strain Z78.

2006
The sequence and annotation of the genome of Herbaspirillum seropedicae strain Z78 is being completed by The Parana State Genome Programme, GENOPAR (www.genopar.org) a consortium of 10 Institutions (UFPR, UEL, UEM, UEPG, UNIOESTE, UNIPAR, IAPAR, EMBRAPA-SOJA, PUC-PR, UFSC). H.
Fábio O. Pedrosa, Genopar Consortium
openaire   +1 more source

Potentiality of Herbaspirillum seropedicae as a Platform for Bioplastic Production

2016
Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate produced by several bacteria as carbon storage and reducing equivalent sink. The production of PHB is a hot topic in biotechnological research due to its properties similar to oil-based plastics, while PHB is readily degradable in the environment.
Marcelo Bueno Batista, Marcelo Müller-Santos, Fábio de Oliveira Pedrosa, Emanuel Maltempi de Souza   +3 more
openaire   +1 more source

Structural organization and regulation of the nif genes of Herbaspirillum seropedicae

Soil Biology and Biochemistry, 1997
Abstract The promoter regions of nifA and nifH genes of Herbaspirillom seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a −24 −12 type promoter and NifA-binding sites; the nifA promoter also has an NtrC-binding site.
F.O. Pedrosa, K.R.S. Teixeira, I.M.P. Machado, M.B.R. Steffens, G. Klassen, E.M. Benelli, H.B. Machado, S. Funayama, L.U. Rigo, M.L. Ishida, M.G. Yates, E.M. Souza   +11 more
openaire   +1 more source

Expression, purification, and DNA-binding activity of the Herbaspirillum seropedicae RecX protein

Protein Expression and Purification, 2004
The Herbaspirillum seropedicae RecX protein participates in the SOS response: a process in which the RecA protein plays a central role. The RecX protein of the H. seropedicae, fused to a His-tag sequence (RecX His-tagged), was over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein.
Carolina W, Galvão, Fábio O, Pedrosa, Emanuel M, Souza, M Geoffrey, Yates, Leda S, Chubatsu, Maria Berenice R, Steffens   +5 more
openaire   +2 more sources

The role of NtrC in the adaptation of Herbaspirillum seropedicae SmR1 to nitrogen limitation and to nitrate

Archives of Microbiology
The RNA-Seq profiling of Herbaspirillum seropedicae SmR1 wild-type and ntrC mutant was performed under aerobic and three nitrogen conditions (ammonium limitation, ammonium shock, and nitrate shock) to identify the major metabolic pathways modulated by these nitrogen sources and those dependent on NtrC. Under ammonium limitation, H.
Paloma, Bonato, Doumit, Camilios-Neto, Michelle Zibetti, Tadra-Sfeir, Francisco J Teles, Mota, Marcelo, Muller-Santos, Roseli, Wassem, Emanuel Maltempi, de Souza, Fábio, de Oliveira Pedrosa, Leda Satie, Chubatsu   +8 more
openaire   +2 more sources