Results 141 to 150 of about 2,167 (179)

Proteomic Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract

Journal of Proteome Research, 2013
Bacterial endophytes of the genus Herbaspirillum colonize sugar cane and can promote plant growth. The molecular mechanisms that mediate plant- H. seropedicae interaction are poorly understood. In this work, we used 2D-PAGE electrophoresis to identify H. seropedicae proteins differentially expressed at the log growth phase in the presence of sugar cane
Michelle Zibetti Tadra, Rose Adele Monteiro, Huergo L F Or Huergo L   +2 more
exaly   +3 more sources

Characterization of the orf1glnKamtB operon of Herbaspirillum seropedicae

Archives of Microbiology, 2005
Herbaspirillum seropedicae is an endophytic nitrogen-fixing bacterium that colonizes economically important grasses. In this organism, the amtB gene is co-transcribed with two other genes: glnK that codes for a PII-like protein and orf1 that codes for a probable periplasmatic protein of unknown function.
Lilian, Noindorf, Fabiane G M, Rego, Valter A, Baura, Rose A, Monteiro, Roseli, Wassem, Leonardo M, Cruz, Liu U, Rigo, Emanuel M, Souza, Maria B R, Steffens, Fabio O, Pedrosa, Leda S, Chubatsu   +10 more
openaire   +2 more sources

Naringenin degradation by the endophytic diazotroph Herbaspirillum seropedicae SmR1

Microbiology, 2013
Several bacteria are able to degrade flavonoids either to use them as carbon sources or as a detoxification mechanism. Degradation pathways have been proposed for several bacteria, but the genes responsible are not known. We identified in the genome of the endophyte Herbaspirillum seropedicae SmR1 an operon potentially associated with the degradation ...
A M, Marin, E M, Souza, F O, Pedrosa, L M, Souza, G L, Sassaki, V A, Baura, M G, Yates, R, Wassem, R A, Monteiro   +8 more
openaire   +2 more sources

Herbaspirillum seropedicae expresses non-phosphorylative pathways for d-xylose catabolism

Applied Microbiology and Biotechnology, 2021
Herbaspirillum seropedicae is a β-proteobacterium that establishes as an endophyte in various plants. These bacteria can consume diverse carbon sources, including hexoses and pentoses like D-xylose. D-xylose catabolic pathways have been described in some microorganisms, but databases of genes involved in these routes are limited.
Ana Karen, Malán, Thalita, Tuleski, Ana Inés, Catalán, Emanuel Maltempi, de Souza, Silvia, Batista   +4 more
openaire   +2 more sources

The PII Protein of Herbaspirillum Seropedicae

1999
The PII protein of Herbaspirillum seropedicae has been purified, crystallized and analysed for physical and biochemical properties. It is trimeric in solution, with a monomeric molecular weight of 12 kDa, comprising 112 amino acid residues, with a C-terminal loop absent from the E. coli PII.
E. M. Benelli, E. M. Souza, M. G. Yates, L. U. Rigo, M. Buck, M. Moore, A. Harper, F. O. Pedrosa   +7 more
openaire   +1 more source

Role of conserved cysteine residues in Herbaspirillum seropedicae NifA activity

Research in Microbiology, 2009
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium that associates with economically important crops. NifA protein, the transcriptional activator of nif genes in H. seropedicae, binds to nif promoters and, together with RNA polymerase-sigma(54) holoenzyme, catalyzes the formation of open complexes to allow transcription initiation.
Marco A S, Oliveira, Valter A, Baura, Bruno, Aquino, Luciano F, Huergo, Marco A S, Kadowaki, Leda S, Chubatsu, Emanuel M, Souza, Ray, Dixon, Fábio O, Pedrosa, Roseli, Wassem, Rose A, Monteiro   +10 more
openaire   +2 more sources

Regulation of Nitrogen Fixation in Herbaspirillum seropedicae

1998
Herbaspirillum seropedicae is an aerobic, plant-associated, nitrogen-fixing bacterium of the β-proteobacteria group. Nitrogen fixation in this organism is repressed by ammonium ions or oxygen, and nitrogenase switch-off by ammonium ions is partial and does not involve ADP-ribosylation (Klasssen et al., unpublished). The nif genes of H.
F. O. Pedrosa, E. M. Souza, E. M. Benelli, I. M. P. Machado, M. B. R. Steffens, G. Klassen, H. B. Machado, L. U. Rigo, R. Wassem, R. A. Monteiro, F. G. M. Rego, L. S. Chubats, M. Buck, M. Moore, A. Harper, M. Drummond, M. G. Yates   +16 more
openaire   +1 more source

In vitro characterization of the NAD+ synthetase NadE1 from Herbaspirillum seropedicae

Archives of Microbiology, 2016
Nicotinamide adenine dinucleotide synthetase enzyme (NadE) catalyzes the amination of nicotinic acid adenine dinucleotide (NaAD) to form NAD(+). This reaction represents the last step in the majority of the NAD(+) biosynthetic routes described to date. NadE enzymes typically use either glutamine or ammonium as amine nitrogen donor, and the reaction is ...
Kerly, Laskoski, Adrian R S, Santos, Ana C, Bonatto, Fábio O, Pedrosa, Emanuel M, Souza, Luciano F, Huergo   +5 more
openaire   +2 more sources

Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae

Journal of Microbiological Methods, 2016
The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values.
Daniella Duarte Villarinho, Pessoa, Marcia Soares, Vidal, José Ivo, Baldani, Jean Luiz, Simoes-Araujo   +3 more
openaire   +2 more sources

A Report on the Genome of Herbaspirillum seropedicae Strain Z78.

2006
The sequence and annotation of the genome of Herbaspirillum seropedicae strain Z78 is being completed by The Parana State Genome Programme, GENOPAR (www.genopar.org) a consortium of 10 Institutions (UFPR, UEL, UEM, UEPG, UNIOESTE, UNIPAR, IAPAR, EMBRAPA-SOJA, PUC-PR, UFSC). H.
Fábio O. Pedrosa, Genopar Consortium
openaire   +1 more source