Results 91 to 100 of about 3,478,631 (289)

Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes [PDF]

, 2008
The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for
Kleerebezem, M., Masja N. Nierop Groot, Michiel Kleerebezem, Jeroen Godefrooij, Nierop Groot, M.N., Godefrooij, J.   +5 more
core   +1 more source

Anisotropic Masked Mycobacterium Potentiates Amplified Antitumor Trained Immunity via Spleen Targeting and Myelopoiesis Conversion

Advanced Materials, EarlyView.
An engineered Trojan Mycobacterium, developed via a “peeling‐off and masking‐up” strategy, functions as a powerful splenic myelopoiesis converter. This spleen‐targeted nanomedicine induces trained immunity to reprogram immunosuppressive myeloid reservoirs into antitumoral effectors.
Jin‐Ho Choi, Sang Nam Lee, Jang Hun Heo, Soyoung Park, Sei Hyun Park, Yong Taik Lim   +5 more
wiley   +1 more source

Heterologous expression system comprising a b. subtilis promoter [PDF]

, 2004
The present invention relates to expression cassettes suitable for the expression of a heterologous gene. Furthermore, the invention relates to recombinant plasmids comprising such expression cassettes.
Kuipers, Oscar Paul; id_orcid, Lindner, Johanna, Hamoen, Leendert   +2 more
core   +4 more sources

Programmable Carrier‐Free All‐Enzyme Beads for Modular Continuous‐Flow Biocatalysis

Advanced Materials, EarlyView.
Genetically encoded enzyme building blocks self‐assemble into monodisperse, carrier‐free protein beads via a droplet‐based formulation strategy. These programmable catalytic particles enable modular continuous‐flow biocatalysis, from single‐enzyme reactions to multi‐enzyme cascades and bead–bead coupled reactor systems.
Jennifer Kühne, Julian S. Hertel, André Delavault, Judith Felk, Martin Peng, Lara Reuber, Felix Ott, Kim Wanner, Marc F. Münker, Adrian Neukirch, Alexei Kiselev, Kersten S. Rabe, Christof M. Niemeyer   +12 more
wiley   +1 more source

Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection

Scientific Reports, 2017
Non-typhoidal Salmonella are associated with gastrointestinal disease worldwide and invasive disease in Africa. We constructed novel bivalent vaccines through the recombinant expression of heterologous O-antigens from Salmonella Choleraesuis in ...
Xinxin Zhao, Qinlong Dai, Dekang Zhu, Mafeng Liu, Shun Chen, Kunfeng Sun, Qiao Yang, Ying Wu, Qingke Kong, Renyong Jia   +9 more
doaj   +1 more source

Alphaviruses ? vectors for the expression of heterologous genes

Trends in Biotechnology, 1993
DNA viruses and retroviruses are well established as vectors for the expression of heterologous genes, but there is increasing interest in the possibilities of using RNA viruses, which do not replicate through a DNA intermediate, for this purpose. This article summarizes some of the general properties of RNA viruses and concentrates on one class of RNA
openaire   +2 more sources

CPP–PEG‐Guided Surface Engineering of Mitochondria Enables Efficient Cellular Uptake and Respiratory Modulation

Advanced Materials Interfaces, EarlyView.
PEG‐guided mitochondrial surface engineering supports structural stabilization and controlled presentation of cell‐penetrating peptides. CPP–PEG‐modified mitochondria exhibit enhanced cellular uptake and uptake‐associated respiratory modulation, providing a proof‐of‐concept framework for modular organelle engineering and future bioenergetic modulation ...
Masahiro Shiraishi, Yukari Muramatsu, Akihiro Nakaya, Yuji Maruo, Rick C. Tsai, Masashi Suganuma, Hisashi Ohta, Atsuhito Takeda, Hideyoshi Harashima, Yuma Yamada   +9 more
wiley   +1 more source

Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]

, 2003
The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
core   +2 more sources

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

Advanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang, Qiang Chen, Aihua Jin, Xiaoqi Yang, Hui Zhou, Weiquan Gong, Jin Cheng, Jun Dai, Minjin Wang, Meihua Piao, Yuanyi Wang, Nan Zhou   +11 more
wiley   +1 more source

The Pisum sativum SAD short-chain dehydrogenase/reductase: quinone reduction, tissue distribution, and heterologous expression [PDF]

, 2011
The pea (Pisum sativum) tetrameric short-chain alcohol dehydrogenase-like protein (SAD) family consists of at least three highly similar members (SAD-A, -B, and -C).
Hilja Strid, Strid, Hilja, Eriksson, Leif A.,, Nikolai Scherbak, Åke Strid, Strid, Hilja,, Anneli Ala-Häivälä, Böwer, Nathalie, Strid, Åke, Grahn, Elin M., Brosché, Mikael, Ala-Häivälä, Anneli, Gittins, John R.,, Elin Grahn, Mikael Brosché, John R. Gittins, Strid, Åke,, Grahn, Elin, Nathalie Böwer, Gittins, John R., Scherbak, Nikolai,, Eriksson, Leif A., Leif A. Eriksson, Grahn, Elin M.,, Scherbak, Nikolai, Brosché, Mikael,   +25 more
core   +1 more source