Results 141 to 150 of about 3,478,631 (289)

Broad‐Spectrum, Cell Envelope‐Active Marinocyclin Antibiotics From a Coral‐Derived Bacterium Are Effective Against Colistin‐Resistant Bacteria

Angewandte Chemie, EarlyView.
Marinocyclins, polycationic macrocyclic depsipeptides, were discovered by genome mining from the marine coral‐derived bacterium Aquimarina megaterium. The compounds have broad‐spectrum activity against gram‐positive and gram‐negative ESKAPE bacteria.
Clara Chepkirui, Sandra Silva, Jan Straetener, Chandrashekhar Padhi, Tina Keller‐Costa, Tess Lamer, Laura Reinke, Heike Brötz‐Oesterhelt, Rodrigo Costa, Jörn Piel   +9 more
wiley   +2 more sources

Molecular Basis for Activation to Inhibition Switching in Kv7.2 Channel Modulators

Angewandte Chemie, EarlyView.
The paper describes the serendipitous discovery of chemical manipulation allowing the activator‐to‐inhibitor switching in Kv7.2 channel modulators. The molecular determinants driving this switch have been rationalized by multidisciplinary investigation encompassing synthetic and analytical chemistry, in silico methods, cryo‐EM analysis ...
Tania Ciaglia, Giusy Carleo, Zhenni Yang, Francesca Di Matteo, Federica De Rosa, Gerardina Smaldone, Zhao‐Bing Gao, Marco D'Alì, Rita Turcio, Demin Ma, Veronica Di Sarno, Nannan Su, Salvatore Vincenzo Giofrè, Giacomo Pepe, Pietro Campiglia, Alessia Bertamino, Francesco Miceli, Jiangtao Guo, Nunzio Iraci, Carmine Ostacolo, Maurizio Taglialatela   +20 more
wiley   +2 more sources

RNA polymerase I promoter and splice acceptor site recognition affect gene expression in non-pathogenic Leishmania species

Memorias do Instituto Oswaldo Cruz, 2007
Leishmania (Sauroleishmania) tarentolae has biotechnological potential for use as live vaccine against visceral leishmaniasis and as a system for the over expression of eukaryotic proteins that possess accurate post-translational modifications.
Tereza Cristina Orlando, Mário Gustavo Mayer, David A Campbell, Nancy R Sturm, Lucile Maria Floeter-Winter   +4 more
doaj  

Gain‐of‐Function Engineering of the NifEN Scaffold Into a Self‐Assembling Nitrogenase

Angewandte Chemie, EarlyView.
From scaffold to enzyme: Systematic engineering of the cofactor maturation scaffold NifEN reconstructs the P→M electron relay along with cofactor coordination and proton gating at the S2B site, converting NifEN into a functional nitrogenase capable of ATP‐dependent N2 reduction both in vitro and in vivo.
Yimo Yang, Chi Chung Lee, Steve Ortiz, Markus W. Ribbe, Yilin Hu   +4 more
wiley   +2 more sources

Adenylation Domain Engineering Enables Biosynthesis of an Improved Polymyxin Antibiotic

Angewandte Chemie, EarlyView.
To enable incorporation of l‐2‐aminodecanoic acid into polymyxin by fermentation, a single Trp‐to‐Ala substitution is rationally introduced into the adenylation‐domain residue of its nonribosomal peptide synthetase (NRPS) and efficiently switches substrate selectivity. The engineered strain produces biologically active Ada‐polymyxin, demonstrating that
Maximilian Müll, Pia Lanvers, Giulia Ravagnan, Kilian Klaus, Olena Nosovska, Ivan Vilotijevic, Jochen Schmid, Hajo Kries   +7 more
wiley   +2 more sources

Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression. [PDF]

Biotechnol J
Vermeulen W, van Staden ADP, Dicks LMT.
europepmc   +1 more source

Heterologous Expression of Thanatin Using Dual Transposon System in <i>Saccharomyces cerevisiae</i>. [PDF]

Biology (Basel)
Li S, Song J, Sun B, Liu K, Li R, Xiang B, Zhang H, Zhao C.   +7 more
europepmc   +1 more source

Heterologous expression of NRPS and PKS pathways in Escherichia coli: from bottlenecks to biosynthetic platforms. [PDF]

Essays Biochem
Göbner L, Gulder TAM, D'Agostino PM.
europepmc   +1 more source

Heterologous expression reveals a cryptic morphogenetic lanthipeptide durhapeptin from Streptomyces durhamensis. [PDF]

Antonie Van Leeuwenhoek
Tsugimoto M, Kobayashi R, Thetsana C, Furukawa T, Nakagawa H, Kodani S.   +5 more
europepmc   +1 more source

Cytotoxicity mechanism of heterologous expression of phospholipase D in <i>Escherichia coli</i>. [PDF]

Microbiol Spectr
Chen S, Wu R, Guo X, Lin S, Lu Y, Ling X.   +5 more
europepmc   +1 more source