Results 61 to 70 of about 145,939 (263)

Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris

Bioengineering
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj   +1 more source

A new high-performance heterologous fungal expression system based on regulatory elements from the Aspergillus terreus terrein gene cluster

Frontiers in Microbiology, 2015
Recently, the Aspergillus terreus terrein gene cluster was identified and selected for development of a new heterologous expression system. The cluster encodes the specific transcription factor TerR that is indispensable for terrein cluster induction. To
Markus eGressler, Peter eHortschansky, Elena eGeib, Matthias eBrock, Matthias eBrock   +4 more
doaj   +1 more source

Chloroplast Stress Signals Orchestrate Epidermis‐Specific Remodeling of Mitochondria and ER Under High Light

Advanced Science, EarlyView.
High light exposure triggers an epidermis‐specific remodeling of mitochondria and ER in Arabidopsis, driven by chloroplast‐derived signals. Live‐cell imaging shows that HL rapidly suppresses mitochondrial motility, followed by fusion‐driven elongation and ER cisternal expansion.
Evan R. Angelos, Hee‐Seung Choi, Jingzhe Guo, Andrea A. Zanini, Tessa M. Burch‐Smith, Emily Snyder, Matthew Part, Wilhelmina van de Ven, Manhoi Hur, Gerd Ulrich Balcke, Alain Tissier, Quanqing Zhang, Katayoon Dehesh   +12 more
wiley   +1 more source

GbWAKL20 Phosphorylates GbNFYB8 to Modulate Verticillium Wilt Resistance in Cotton

Advanced Science, EarlyView.
Wall‐associated receptor‐like kinases (WAKLs) play pivotal roles in extracellular–intracellular signal transduction. Upon sensing Verticillium dahliae infestation at the plasma membrane, GbWAKL20 accumulates and transmits signals to the nucleus via endoplasmic reticulum‐mediated Golgi vesicle transport.
Guilin Wang, Qingxin Si, Zhiguo Chen, Zhe Yu, Zhan Guo, Lu Wang, Weixi Li, Wangzhen Guo   +7 more
wiley   +1 more source

A Testis‐Specific Aralkylamine N‐Acetyltransferase Regulates Dimorphic Sperm Function and Male Fertility in Moths

Advanced Science, EarlyView.
We identify a Lepidoptera‐conserved testis‐specific arylalkylamine N‐acetyltransferase (LTNAT) that governs male moth fertility via a novel mechanism. LTNAT loss disrupts eupyrene sperm mitochondrial derivatives and impairs apyrene sperm motility, offering a safe molecular target for innovative pesticides and genetic pest control.
Hao Sun, Peng‐Yi Huang, Zhi‐Ruo Zhang, Cong‐Fen Gao, Subba Reddy Palli, Shun‐Fan Wu   +5 more
wiley   +1 more source

Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection

Scientific Reports, 2017
Non-typhoidal Salmonella are associated with gastrointestinal disease worldwide and invasive disease in Africa. We constructed novel bivalent vaccines through the recombinant expression of heterologous O-antigens from Salmonella Choleraesuis in ...
Xinxin Zhao, Qinlong Dai, Dekang Zhu, Mafeng Liu, Shun Chen, Kunfeng Sun, Qiao Yang, Ying Wu, Qingke Kong, Renyong Jia   +9 more
doaj   +1 more source

Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity

Journal of Pure and Applied Microbiology, 2017
Three lipase genes namely LK2, LK3 and LK5 were successfully sub-cloned into expression vector pET30a. The recombinant vectors were heterologically expressed into Escherichia coli BL21 (DE3) by 1 mM IPTG induced at 37 °C.
Nurhasanah, Santi Nurbaiti, Fida Madayanti, Akhmaloka   +3 more
doaj   +1 more source

Metabologenomic Hallmark‐Based Discovery of Bacterial Thioamides as a New Lead against Drug‐Resistant Pancreatic Cancer

Advanced Science, EarlyView.
A targeted metabologenomic strategy integrating genomic and metabolomic hallmarks enabled the discovery of two new thioamides, thiogochangamides A and B, from bacteria. Their absolute configurations, previously unresolved within the thioviridamide family, were fully assigned through various chemical derivatizations and computational analysis ...
Young Eun Du, Eun Seo Bae, Thinh T. M. Bui, Seok Beom Lee, Dahan Kim, Jin‐Gyeong Park, Yongjoo Park, Soo Yeon Park, Sangwook Kang, Beomsu Lee, Daniel Shin, Yun Pyo Kang, In‐Gyun Lee, Dae‐Duk Kim, Seokhee Kim, Suckchang Hong, Kyuho Moon, Sang Kook Lee, Dong‐Chan Oh   +18 more
wiley   +1 more source

PD‐L1‐Binding Antigen Presenters: Redirecting Vaccine‐Induced Antibodies for Cancer Immunotherapy

Advanced Science, EarlyView.
The PBAP‐gE complex anchors gE antigen to PD‐L1 on tumor cells. Vaccine‐induced anti‐gE antibodies simultaneously engage FcγRIIIa on NK cells and tumor‐bound PBAP‐gE, triggering NK cell activation and antibody‐dependent cellular cytotoxicity, thereby selectively eliminating PD‐L1–expressing tumor cells.
Huixin Gao, Lijuan Lu, Xiaoxiao Xiong, Yi Li, Donghui Hu, Duo Zhang, Zhiwei Feng, Cong Liu, Nannan Liu, Xiaoli Li, Jizhou Tan, Ting Liu, Ling Peng, Lu Lu, Huiyi Feng, Yan Zhong, Guisen Tan, Zhicheng Zhang, Liqin Huang, Chao Su, Ying Xue, Shuo Song, Wenxia Fan, Wei Wang, Fan Zou   +24 more
wiley   +1 more source

Heterologous expression and characterisation of microcystinase

Toxicon, 2012
The first enzyme in the microcystin (MC) degradation pathway identified in bacterial strains is coded by mlrA gene and is referred to as microcystinase. To date, there has been no biochemical characterisation of this enzyme. The results presented herein show a successful heterologous expression of MlrA as well as mutational studies, partial ...
Dziga, Dariusz, Władyka, Benedykt, Zielińska, Gabriela, Meriluoto, Jussi, Wasylewski, Marcin   +4 more
openaire   +3 more sources