Results 41 to 50 of about 3,478,631 (289)

Altered fatty acid composition of Nicotiana benthamiana and Nicotiana excelsior leaves under transient overexpression of the cyanobacterial desC gene

Biologia Plantarum, 2020
Transient heterologous gene expression in two model plant species, Nicotiana benthamiana and N. excelsior, has been used to study the localization of the heterologous Δ9 acyl-lipid desaturase (Δ9 desaturase) of Synechococcus vulcanus in different cell ...
M. BERESTOVOY, O.S. PAVLENKO, A.A. TYURIN, E.N. GORSHKOVA, I.V. GOLDENKOVA-PAVLOVA   +4 more
doaj   +1 more source

Heterologous expression and protein engineering of wheat gluten proteins

, 2006
A range of systems are available for the production of recombinant wheat gluten proteins, from simple and widely used systems based on Escherichia coli to more sophisticated eukaryotic systems in yeasts or cultured insect cells. The characteristics of
Tamas, L., Tamás, László, Shewry, P. R.   +2 more
core   +1 more source

Heterologous expression facilitates the discovery and characterization of marine microbial natural products

Engineering Microbiology
Microbial natural products and their derivatives have been developed as a considerable part of clinical drugs and agricultural chemicals. Marine microbial natural products exhibit diverse chemical structures and bioactivities with substantial potential ...
Shuang Zhao, Ruiying Feng, Yuan Gu, Liyuan Han, Xiaomei Cong, Yang Liu, Shuo Liu, Qiyao Shen, Liujie Huo, Fu Yan   +9 more
doaj   +1 more source

Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems

Frontiers in Pharmacology, 2023
Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to ...
Nicolás Lefin, Javiera Miranda, Jorge F. Beltrán, Lisandra Herrera Belén, Brian Effer, Adalberto Pessoa, Jorge G. Farias, Mauricio Zamorano   +7 more
doaj   +1 more source

Discovery, Isolation, Heterologous Expression and Mode of Action Studies of the Antibiotic Polyketide Tatiomicin from Amycolatopsis sp. DEM30355 [PDF]

, 2022
A genomic and bioactivity informed analysis of the metabolome of the extremophile Amycolatopsis sp. DEM30355 has allowed for the discovery and isolation of the polyketide antibiotic tatiomicin.
Michael, Goodfellow, Bill, Clegg, John, Perry, Lina, Mardiana, Stephanie, Morton-Laing, Michael, Probert, Joe, Grey, Jonathan, Bogaerts, Wouter, Herrebout, Corinne, Wills, Yousef, Dashti, Jeff, Errington, Nick E. E., Allenby, Emma, Marrs, Michael, Hall, Joseph, Cowell, Bernhard, Kepplinger   +16 more
core   +1 more source

Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris

Biotechnology for Biofuels, 2019
Background Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis.
Xihao Liao, Jing Zhao, Shuli Liang, Jingjie Jin, Cheng Li, Ruiming Xiao, Lu Li, Meijin Guo, Gong Zhang, Ying Lin   +9 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

A simple, highly efficient method for heterologous expression in mammalian primary neurons using cationic lipid-mediated mRNA transfection

Frontiers in Neuroscience, 2010
Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The postmitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods.
Damian J Williams, Henry L Puhl, Stephen R Ikeda   +2 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source