Results 21 to 30 of about 3,478,631 (289)

ENHANCED HETEROLOGOUS EXPRESSION IN E.COLI

Studia Universitatis Babes-Bolyai Chemia, 2019
Apoptotic regulation has been implicated in many human diseases, including cancer, autoimmune disease, inflammation and neuro degradation. Mapping up critical apoptosis regulators is a strategy for the development of new therapies [1, 2].
Katalin NAGY, Zita KOVÁCS, Pál SALAMON, Csongor-Kálmán ORBÁN, Szabolcs LÁNYI, Beáta ALBERT   +5 more
doaj   +1 more source

The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae [PDF]

, 2011
Protein synthesis translates information from messenger RNAs into functional proteomes. Because of the finite nature of the resources required by the translational machinery, both the overall protein synthesis activity of a cell and activity on ...
von der Haar, Tobias, David J. Barnes, Barnes, David J., Dominique Chu, Tobias von der Haar, Chu, Dominique   +5 more
core   +1 more source

Heterologous expression.

, 2022
A) Heterologous expression of plasmids encoding eGFP tagged PRM2 (Prm2-eGFP), mP2 (mP2-eGFP) or eGFP (pEGFP-N3) in human embryonic kidney 293 (HEK) cells 48 hours post-transfection. Scale bar = 50μm. B) Western blot against GFP (Anti-GFP antibody ab6556,
Lena Arévalo (8148903), Simon Schneider (749180), Isabelle Sophie Neumann (12969420), Hubert Schorle (15757), Gina Esther Merges (12969414), Franka Enow Oben (12969417)   +5 more
core   +1 more source

Exploring the biocombinatorial potential of benzoxazoles: generation of novel caboxamycin derivatives

Microbial Cell Factories, 2017
Background The biosynthesis pathway of benzoxazole compounds caboxamycin and nataxazole have been recently elucidated. Both compounds share one of their precursors, 3-hydroxyanthranilate (two units in the case of nataxazole).
Armando A. Losada, Carmen Méndez, José A. Salas, Carlos Olano   +3 more
doaj   +1 more source

A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization

Electronic Journal of Biotechnology, 2014
Background: Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic ...
Lei Wei, Li Yin, Xiaole Hu, Ying Xu, Fang Chen   +4 more
doaj   +1 more source

Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters

Microbial Cell Factories, 2020
Background Heterologous expression of secondary metabolite gene clusters is used to achieve increased production of desired compounds, activate cryptic gene clusters, manipulate clusters from genetically unamenable strains, obtain natural products from ...
Yousra Ahmed, Yuriy Rebets, Marta Rodríguez Estévez, Josef Zapp, Maksym Myronovskyi, Andriy Luzhetskyy   +5 more
doaj   +1 more source

Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21.

Revista Colombiana de Biotecnología, 2020
Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle, Gloria Maria Restrepo Franco, Narmer Fernando Galeano Vanegas   +2 more
doaj   +1 more source

Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum

Microbial Cell Factories, 2021
Background Ganoderma lucidum, a well-known medicinal mushroom, has received wide attention as a promising cell factory for producing bioactive compounds.
Hao You, Bin Sun, Na Li, Jun-Wei Xu
doaj   +1 more source

Heterologous expression from the human D-Loop in organello [PDF]

, 2014
We report the expression of a linear reporter construct in isolated human mitochondria. The reporter construct contained the entire human D-Loop with adjacent tRNA (MTT) genes (mt.15956-647), the human ND1 gene with an in frame GFP gene and adjacent ...
Schaller, André, Jackson, Christopher, Gallati, Sabina, Zbinden, C   +3 more
core   +1 more source

Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin in Aspergillus niger

Journal of Fungi, 2023
Aspergillus niger is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current A.
Ke Li, Junwei Zheng, Leyi Yu, Bin Wang, Li Pan   +4 more
doaj   +1 more source