Results 21 to 30 of about 149,241 (263)

Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum

open access: yesMicrobial Cell Factories, 2021
Background Ganoderma lucidum, a well-known medicinal mushroom, has received wide attention as a promising cell factory for producing bioactive compounds.
Hao You, Bin Sun, Na Li, Jun-Wei Xu
doaj   +1 more source

Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin in Aspergillus niger

open access: yesJournal of Fungi, 2023
Aspergillus niger is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current A.
Ke Li   +4 more
doaj   +1 more source

18-Hydroxydolabella-3,7-diene synthase – a diterpene synthase from Chitinophaga pinensis

open access: yesBeilstein Journal of Organic Chemistry, 2017
The product obtained in vitro from a diterpene synthase encoded in the genome of the bacterium Chitinophaga pinensis, an enzyme previously reported to have germacrene A synthase activity during heterologous expression in Escherichia coli, was identified ...
Jeroen S. Dickschat   +4 more
doaj   +1 more source

Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

open access: yesmBio, 2017
Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram ...
Jia Jia Zhang   +4 more
doaj   +1 more source

Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. [PDF]

open access: yesPLoS ONE, 2012
Microalgae have recently received attention as a potential low-cost host for the production of recombinant proteins and novel metabolites. However, a major obstacle to the development of algae as an industrial platform has been the poor expression of ...
Beth A Rasala   +5 more
doaj   +1 more source

Heterologous systems for expression of mammalian sulfotransferases [PDF]

open access: yesChemico-Biological Interactions, 1994
This paper describes the use of both mammalian and bacterial expression systems as tools to study the structural and functional relationships of proteins encoded by cDNAs to both rat and human aryl sulfotransferases. In particular, we describe the use of the mammalian COS cell system for functional expression studies, and the use of Escherichia coli ...
Veronese, M. E.   +5 more
openaire   +5 more sources

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

open access: yesMicrobial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Production of Recombinant Biopharmaceuticals in Chlamydomonas reinhardtii

open access: yesInternational Journal of Plant Biology, 2022
This review aimed to present Chlamydomonas reinhardtii as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous
Cesar Andres Diaz Arias   +5 more
doaj   +1 more source

Altered fatty acid composition of Nicotiana benthamiana and Nicotiana excelsior leaves under transient overexpression of the cyanobacterial desC gene

open access: yesBiologia Plantarum, 2020
Transient heterologous gene expression in two model plant species, Nicotiana benthamiana and N. excelsior, has been used to study the localization of the heterologous Δ9 acyl-lipid desaturase (Δ9 desaturase) of Synechococcus vulcanus in different cell ...
M. BERESTOVOY   +4 more
doaj   +1 more source

Heterologous expression and characterisation of microcystinase

open access: yesToxicon, 2012
The first enzyme in the microcystin (MC) degradation pathway identified in bacterial strains is coded by mlrA gene and is referred to as microcystinase. To date, there has been no biochemical characterisation of this enzyme. The results presented herein show a successful heterologous expression of MlrA as well as mutational studies, partial ...
Dziga, Dariusz   +4 more
openaire   +3 more sources

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