Results 1 to 10 of about 149,241 (263)
Modelling hCDKL5 Heterologous Expression in Bacteria [PDF]
Metabolites, 2021 hCDKL5 refers to the human cyclin-dependent kinase like 5 that is primarily expressed in the brain. Mutations in its coding sequence are often causative of hCDKL5 deficiency disorder, a devastating neurodevelopmental disorder currently lacking a cure ...
Marco Fondi +9 more
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Marine Drugs, 2022 Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms.
Yushan Xu +6 more
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Fermentation, 2023 AA10 family lytic polysaccharide monooxygenases (AA10 LPMOs) are mainly distributed in bacteria. Because of their characteristics of oxidative degradation of crystalline polysaccharides, such as cellulose and chitin, they have great application potential
Hongyu Zhang +6 more
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Alternative sigma factor over-expression enables heterologous expression of a type II polyketide biosynthetic pathway in Escherichia coli. [PDF]
PLoS ONE, 2013 BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways.
David Cole Stevens +5 more
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mBio, 2023 The functional versatility of the Fe protein, the reductase component of nitrogenase, makes it an appealing target for heterologous expression, which could facilitate future biotechnological adaptations of nitrogenase-based production of valuable ...
Joseph B. Solomon +7 more
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Heterologous Expression of Limulus Rhodopsin [PDF]
Journal of Biological Chemistry, 2003 Invertebrates such as Drosophila or Limulus assemble their visual pigment into the specialized rhabdomeric membranes of photoreceptors where phototransduction occurs. We have investigated the biosynthesis of rhodopsin from the Limulus lateral eye with three cell culture expression systems: mammalian COS1 cells, insect Sf9 cells, and amphibian Xenopus ...
Barry E, Knox +8 more
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Heterologous expression of human transketolase [PDF]
The International Journal of Biochemistry & Cell Biology, 1998 Transketolase belongs to the family of thiamin diphosphate dependent enzymes. The aim of this study was to establish a bacterial expression system for human transketolase in order to investigate the functional characteristics of mammalian transketolases. The level of recombinant human enzyme expressed in Escherichia coli was modest.
Schenk, Gerhard +2 more
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Microorganisms, 2020 Since the 1950s, natural products of bacterial origin were systematically developed to be used as drugs with a wide range of medical applications. The available treatment options for many diseases are still not satisfying, wherefore, the discovery of new
Constanze Lasch +5 more
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Reconstitution of Polyketide-Derived Meroterpenoid Biosynthetic Pathway in Aspergillus oryzae
Journal of Fungi, 2021 The heterologous gene expression system with Aspergillus oryzae as the host is an effective method to investigate fungal secondary metabolite biosynthetic pathways for reconstruction to produce un-natural molecules due to its high productivity and ...
Takayoshi Awakawa, Ikuro Abe
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Heterologous expression of cryptomaldamide in a cyanobacterial host [PDF]
ACS Synthetic Biology, 2020 ABSTRACT Filamentous marine cyanobacteria make a variety of bioactive molecules that are produced by polyketide synthases, non-ribosomal peptide synthetases, and hybrid pathways that are encoded by large biosynthetic gene clusters.
Arnaud Taton +11 more
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