Results 31 to 40 of about 149,241 (263)

Heterologous expression facilitates the discovery and characterization of marine microbial natural products

Engineering Microbiology
Microbial natural products and their derivatives have been developed as a considerable part of clinical drugs and agricultural chemicals. Marine microbial natural products exhibit diverse chemical structures and bioactivities with substantial potential ...
Shuang Zhao, Ruiying Feng, Yuan Gu, Liyuan Han, Xiaomei Cong, Yang Liu, Shuo Liu, Qiyao Shen, Liujie Huo, Fu Yan   +9 more
doaj   +1 more source

Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems

Frontiers in Pharmacology, 2023
Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to ...
Nicolás Lefin, Javiera Miranda, Jorge F. Beltrán, Lisandra Herrera Belén, Brian Effer, Adalberto Pessoa, Jorge G. Farias, Mauricio Zamorano   +7 more
doaj   +1 more source

Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris

Biotechnology for Biofuels, 2019
Background Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis.
Xihao Liao, Jing Zhao, Shuli Liang, Jingjie Jin, Cheng Li, Ruiming Xiao, Lu Li, Meijin Guo, Gong Zhang, Ying Lin   +9 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

A simple, highly efficient method for heterologous expression in mammalian primary neurons using cationic lipid-mediated mRNA transfection

Frontiers in Neuroscience, 2010
Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The postmitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods.
Damian J Williams, Henry L Puhl, Stephen R Ikeda   +2 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Heterologous Expression and Transglycosylation of Acarviosyltransferase [PDF]

Shipin Kexue
In order to further investigate the structural properties and catalytic function of acarviosyltransferase (ATase), a key enzyme in the biosynthesis of acarbose, its gene (acbD) was cloned from Actinoplanes sp.
XUE Zhenglian, WANG Yuqing, LI Chuang, LI Dandan, ZHU Sibao, LI Xiangfei
doaj   +1 more source

Cloning and expression of Burkholderia polyyne biosynthetic gene clusters in Paraburkholderia hosts provides a strategy for biopesticide development

Microbial Biotechnology, 2022
Burkholderia have potential as biocontrol agents because they encode diverse biosynthetic gene clusters (BGCs) for a range of antimicrobial metabolites.
Yoana D. Petrova, Jinlian Zhao, Gordon Webster, Alex J. Mullins, Katherine Williams, Amal S. Alswat, Gregory L. Challis, Andy M. Bailey, Eshwar Mahenthiralingam   +8 more
doaj   +1 more source