Results 31 to 40 of about 3,478,631 (289)

18-Hydroxydolabella-3,7-diene synthase – a diterpene synthase from Chitinophaga pinensis

Beilstein Journal of Organic Chemistry, 2017
The product obtained in vitro from a diterpene synthase encoded in the genome of the bacterium Chitinophaga pinensis, an enzyme previously reported to have germacrene A synthase activity during heterologous expression in Escherichia coli, was identified ...
Jeroen S. Dickschat, Jan Rinkel, Patrick Rabe, Arman Beyraghdar Kashkooli, Harro J. Bouwmeester   +4 more
doaj   +1 more source

Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

mBio, 2017
Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram ...
Jia Jia Zhang, Xiaoyu Tang, Michelle Zhang, Darlene Nguyen, Bradley S. Moore   +4 more
doaj   +1 more source

Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. [PDF]

PLoS ONE, 2012
Microalgae have recently received attention as a potential low-cost host for the production of recombinant proteins and novel metabolites. However, a major obstacle to the development of algae as an industrial platform has been the poor expression of ...
Beth A Rasala, Philip A Lee, Zhouxin Shen, Steven P Briggs, Michael Mendez, Stephen P Mayfield   +5 more
doaj   +1 more source

Expression of Lectins in Heterologous Systems [PDF]

International Journal of Molecular Sciences, 2018
Lectins are proteins that have the ability to recognize and bind in a reversible and specific way to free carbohydrates or glycoconjugates of cell membranes. For these reasons, they have been extensively used in a wide range of industrial and pharmacological applications.
Dania Martínez-Alarcón, Alejandro Blanco-Labra, Teresa García-Gasca   +2 more
openaire   +2 more sources

Synthetic biology of fungal natural products [PDF]

, 2015
This work was supported by the DFG-funded excellence graduate school Jena School for Microbial Communication (JSMC; DJM, AAB), the collaborative research center (SFB) 1127 Chemical Mediators in Complex Biosystems (ChemBioSys—AAB, project B02) and the ...
Vito Valiante, Unkles, S.E., Mattern, D.J., Valiante, V., Brakhage, A.A., Derek J. Mattern, Axel A. Brakhage, Shiela E. Unkles   +7 more
core   +1 more source

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Microbial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Recombinant expression of fungal oxidases for industrial application [PDF]

, 2005
Laccases catalyse the oxidation of a range of organic substrates coupled to the reduction of molecular oxygen to water. They are members of the ubiquitous blue multi-copper oxidase family.
Piscitelli, Alessandra
core   +1 more source

Production of Recombinant Biopharmaceuticals in Chlamydomonas reinhardtii

International Journal of Plant Biology, 2022
This review aimed to present Chlamydomonas reinhardtii as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous
Cesar Andres Diaz Arias, Caroline Frere Martiniuc de Oliveira, João Vitor Dutra Molino, Livia Seno Ferreira-Camargo, Marcelo Chuei Matsudo, João Carlos Monteiro de Carvalho   +5 more
doaj   +1 more source

Heterologous systems for expression of mammalian sulfotransferases [PDF]

Chemico-Biological Interactions, 1994
This paper describes the use of both mammalian and bacterial expression systems as tools to study the structural and functional relationships of proteins encoded by cDNAs to both rat and human aryl sulfotransferases. In particular, we describe the use of the mammalian COS cell system for functional expression studies, and the use of Escherichia coli ...
Veronese, M. E., Burgess, W., Cruickshank, D., Sansom, L., Zhu, X., McManus, M. E.   +5 more
openaire   +5 more sources

Heterologous expression of the transcription factors AtfA and AtfB in Escherichia coli

, 2023
The aim of my thesis is to extract desired biological product through heterologous expression in Escherichia Coli. The study focuses on the bZip transcriptional factors atfA and atfB and their subsequent purification through heterologous expresson.
Ullah, Zain
core