Results 11 to 20 of about 149,241 (263)

Construction of T7-Like Expression System in Pseudomonas putida KT2440 to Enhance the Heterologous Expression Level

open access: yesFrontiers in Chemistry, 2021
Pseudomonas putida KT2440 has become an attractive chassis for heterologous expression with the development of effective genetic manipulation tools. Improving the level of transcriptional regulation is particularly important for extending the potential ...
Tianxin Liang   +8 more
doaj   +1 more source

Xenopus Oocytes: A Tool to Decipher Molecular Specificity of Insecticides towards Mammalian and Insect GABA—A Receptors

open access: yesMembranes, 2022
The number of insect GABA receptors (GABAr) available for expression studies has been recently increased by the cloning of the Acyrthosiphon pisum (pea aphid) RDL subunits.
Anaïs Bertaud   +10 more
doaj   +1 more source

A set of vectors and strains for chromosomal integration in fission yeast

open access: yesScientific Reports, 2023
The expression of heterologous genes is an important technique in yeast genetics. In fission yeast, the leu1 and ura4 genes have been used mainly as selectable markers for heterologous expression.
Akihisa Matsuyama   +3 more
doaj   +1 more source

Expression of Lectins in Heterologous Systems [PDF]

open access: yesInternational Journal of Molecular Sciences, 2018
Lectins are proteins that have the ability to recognize and bind in a reversible and specific way to free carbohydrates or glycoconjugates of cell membranes. For these reasons, they have been extensively used in a wide range of industrial and pharmacological applications.
Dania Martínez-Alarcón   +2 more
openaire   +2 more sources

Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters

open access: yesFrontiers in Microbiology, 2018
Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover “cryptic” secondary metabolites. To improve the heterologous expression of BGCs, herein we optimized S.
Qinying Peng   +11 more
doaj   +1 more source

ENHANCED HETEROLOGOUS EXPRESSION IN E.COLI

open access: yesStudia Universitatis Babes-Bolyai Chemia, 2019
Apoptotic regulation has been implicated in many human diseases, including cancer, autoimmune disease, inflammation and neuro degradation. Mapping up critical apoptosis regulators is a strategy for the development of new therapies [1, 2].
Katalin NAGY   +5 more
doaj   +1 more source

A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization

open access: yesElectronic Journal of Biotechnology, 2014
Background: Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic ...
Lei Wei   +4 more
doaj   +1 more source

Exploring the biocombinatorial potential of benzoxazoles: generation of novel caboxamycin derivatives

open access: yesMicrobial Cell Factories, 2017
Background The biosynthesis pathway of benzoxazole compounds caboxamycin and nataxazole have been recently elucidated. Both compounds share one of their precursors, 3-hydroxyanthranilate (two units in the case of nataxazole).
Armando A. Losada   +3 more
doaj   +1 more source

Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters

open access: yesMicrobial Cell Factories, 2020
Background Heterologous expression of secondary metabolite gene clusters is used to achieve increased production of desired compounds, activate cryptic gene clusters, manipulate clusters from genetically unamenable strains, obtain natural products from ...
Yousra Ahmed   +5 more
doaj   +1 more source

Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21.

open access: yesRevista Colombiana de Biotecnología, 2020
Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle   +2 more
doaj   +1 more source

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