Results 11 to 20 of about 3,478,631 (289)
A heterologous expression system for bovine lens transmembrane main intrinsic protein (MIP) in Nicotiana tabacum plants [PDF]
, 1999 We have developed a heterologous expression system for transmembrane lens main intrinsic protein (MIP) in Nicotiana tabacum plant tissue. A native bovine MIP26 amplicon was subcloned into an expression cassette under the control of a constitutive ...
Dunwell, Jim M. +3 more
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Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity
Journal of Pure and Applied Microbiology, 2017 Three lipase genes namely LK2, LK3 and LK5 were successfully sub-cloned into expression vector pET30a. The recombinant vectors were heterologically expressed into Escherichia coli BL21 (DE3) by 1 mM IPTG induced at 37 °C.
Nurhasanah +3 more
doaj +2 more sources
Bacterial natural product discovery by heterologous expression [PDF]
, 2023 Natural products have found important applications in the pharmaceutical and agricultural sectors. In bacteria, the genes that encode the biosynthesis of natural products are often colocalized in the genome, forming biosynthetic gene clusters.
Adjo, Kadjo, Alessandra, Eustaquio
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Identification, heterologous expression, and characterization of the tolypodiol biosynthetic gene cluster through an integrated approach. [PDF]
, 2023 Cyanobacteria are tremendous producers of biologically active natural products including the potent anti-inflammatory compound tolypodiol. However, linking biosynthetic gene clusters with compound production in cyanobacteria has lagged behind other ...
Philip , Williams +6 more
core +2 more sources
Heterologous expression of human transketolase [PDF]
The International Journal of Biochemistry & Cell Biology, 1998 Transketolase belongs to the family of thiamin diphosphate dependent enzymes. The aim of this study was to establish a bacterial expression system for human transketolase in order to investigate the functional characteristics of mammalian transketolases. The level of recombinant human enzyme expressed in Escherichia coli was modest.
Schenk, Gerhard +2 more
openaire +6 more sources
Frontiers in Chemistry, 2021 Pseudomonas putida KT2440 has become an attractive chassis for heterologous expression with the development of effective genetic manipulation tools. Improving the level of transcriptional regulation is particularly important for extending the potential ...
Tianxin Liang +8 more
doaj +1 more source
Membranes, 2022 The number of insect GABA receptors (GABAr) available for expression studies has been recently increased by the cloning of the Acyrthosiphon pisum (pea aphid) RDL subunits.
Anaïs Bertaud +10 more
doaj +1 more source
A set of vectors and strains for chromosomal integration in fission yeast
Scientific Reports, 2023 The expression of heterologous genes is an important technique in yeast genetics. In fission yeast, the leu1 and ura4 genes have been used mainly as selectable markers for heterologous expression.
Akihisa Matsuyama +3 more
doaj +1 more source
Use of homologous and heterologous gene expression profiling tools to characterize transcription dynamics during apple fruit maturation and ripening [PDF]
, 2010 Background Fruit development, maturation and ripening consists of a complex series of biochemical and physiological changes that in climacteric fruits, including apple and tomato, are coordinated by the gaseous hormone ethylene.
Valeria Soglio +56 more
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Frontiers in Microbiology, 2018 Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover “cryptic” secondary metabolites. To improve the heterologous expression of BGCs, herein we optimized S.
Qinying Peng +11 more
doaj +1 more source