Results 61 to 70 of about 3,478,631 (289)

Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Microbial Cell Factories, 2023
Background Lanthipeptides are a rapidly expanding family of ribosomally synthesized and post-translationally modified natural compounds with diverse biological functions.
Winschau F Van Zyl, Anton D. Van Staden, Leon M T. Dicks, Marla Trindade   +3 more
doaj   +1 more source

Cooperativity of Glucocorticoid Response Elements Located Far Upstream of the Tyrosine Aminotransferase Gene [PDF]

, 1987
Two glucocorticoid response elements (GREs) located 2.5 kb upstream of the transcription initiation site of the tyrosine aminotransferase gene were identified by gene transfer experiments and shown to bind to purified glucocorticoid receptor.
Strähle, Uwe, Boshart, Michael, Schmid, Wolfgang, Miksicek, Richard, Jansen, Hans-Michael, Gloss, Bernd, Schlitz, Günther, Stewart, Francis   +7 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome

Microbial Cell Factories, 2023
Background In the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct an efficient exocrine expression system for the different heterologous proteins.
Siqian Yu, Ge Zhang, Qi Liu, Yingping Zhuang, Zongjie Dai, Jianye Xia   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Engineering of Microbial Expression Systems for the In Vitro Production of Anticancer Peptides

Proceedings
Introduction: Cancer causes nearly 10 million deaths worldwide each year, with approximately 704,000 new cases in Brazil [...]
Giovana A. Vasconcelos, Pedro E. B. Guedes, Thais M. M. Ferreira, Victor A. Cunha, André R. C. Junior, Octavio L. Franco, Karen O. Osiro   +6 more
doaj   +1 more source

Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host. [PDF]

PLoS ONE, 2008
Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence. During heterologous protein expression, low expression or formation of insoluble aggregates may be attributable to ...
Evelina Angov, Collette J Hillier, Randall L Kincaid, Jeffrey A Lyon   +3 more
doaj   +1 more source

Potential of Synechocystis PCC 6803 as a novel cyanobacterial chassis for heterologous expression of enzymes in the trans-resveratrol biosynthetic pathway

, 2016
Selected model strains of phototrophic cyanobacteria have been genetically engineered for heterologous expression of numerous enzymes. In the present study, we initially explored the heterologous expression of enzymes involved in trans-resveratrol ...
Incharoensakdi, Aran,, Lindblad, Peter,, Tantong, Supaluk,, Sirikantaramas, Supaart,   +3 more
core   +1 more source

Heterologous expression of the yeast arsenite efflux system ACR3 improves Arabidopsis thaliana tolerance to arsenic stress [PDF]

, 2012
Arsenic contamination has a negative impact on crop cultivation and on human health. As yet, no proteins have been identified in plants that mediate the extrusion of arsenic. Here, we heterologously expressed the yeast (Saccharomyces cerevisiae) arsenite
Maathuis, F. J. M., Ali, W., Zhao, F-J., Isner, J-C., Liu, W., Isayenkov, S. V.   +5 more
core   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source