Results 141 to 150 of about 28,492 (307)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Frequency Domain Modeling of High Voltage Transformers Using a Nonlinear Least-Square Estimation Technique [PDF]
, 2009 The development of transformer models can be achieved based on experimental frequency response measurements providing access to the windings is available and the physical dimensions are known in order to calculate R, L and C parameter values. Of interest
Mohamed, R, Lewin, P L
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Simulation of a transformer winding for partial discharge propagation studies [PDF]
, 2002 A simulation model of a continuous disc type 6.6 kV transformer winding was used to study the propagation behaviour of partial discharge (PD) pulses. The model based on multi-conductor transmission line theory uses a single turn as a circuit element with
Darwin, A. +4 more
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
High Frequency Transformer : Implementation of prototype
, 2019 Since its invention in 1885 by Otto Bláthy, Miksa Déri and KárolyZipernowsky, transformers have become an important cornerstone of theelectrical infrastructure we have today. They are found mostprominently in any machinery or device that requires a differentlevel of voltage or current than a general grid can supply, such ascomputers, motors or even ...
Jansson, Vincent +2 more
openaire +1 more source
Soft Magnetic Materials for High Frequency High Power Density Transformers in Power Electronic Systems [PDF]
, 2007 Along with the trends of higher and higher frequency operations and smaller and smaller physical volumes of power electronic systems, the transformers and inductors used in the power electronic systems are facing challenge to operate at high frequencies.
Zhong, J, Jin, J, Zhu, J, Lu, H, Guo, Y
core
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source