Artificial Receptor in Synthetic Cells Performs Transmembrane Activation of Proteolysis
Transmembrane signaling is the hallmark of living cells and is among the highest challenges for the design of synthetic cells. Herein, an artificial receptor based on the chemistry of self‐immolative linkers is used to communicate information across the lipid bilayer, for transmembrane activation of enzymatic activity. Abstract The design of artificial,
Ane Bretschneider Søgaard+7 more
wiley +1 more source
Spatiotemporal Control Over Protein Release from Artificial Cells via a Light‐Activatable Protease
Stimulus‐responsive protein release is essential for intercellular communication. Mimicking this functionality in artificial cells is promising to study the working principles of cellular signaling. Herein, an engineered light‐activatable protease is implemented in a coacervate‐based artificial cell platform to establish user‐defined spatiotemporal ...
Arjan Hazegh Nikroo+4 more
wiley +1 more source
High Performance Liquid Chromatography for the Detection of Homozygotes and Heterozygotes of Niemann-Pick Disease [PDF]
Firoze B. Jungalwala, Aubrey Milunsky
openalex +1 more source
Stability studies of coal liquid products using high performance liquid chromatography [PDF]
The storage stability of coal liquids is of great importance if coal liquefaction products are to replace petroleum products. It has been reported that gum formation occurs in coal-derived liquids during storage to a level at which it becomes a handling ...
Norcio, Lawrence P.
core +1 more source
Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells
TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter+3 more
wiley +1 more source
Antibiotics are a common practice in veterinary medicine, mainly for therapeutic purposes. Sectors of application include livestock farming, aquacultures, and bee-keeping, where bacterial infections are frequent and can be economically damaging. However,
Dimitrios Bitas+3 more
doaj +1 more source
A Preliminary Study of Oxidation of Lignin from Rubber Wood to Vanillin in Ionic Liquid Medium [PDF]
In this study, lignin was oxidised to vanillin by means of oxygen in ionic liquid (1,3-dimethylimidazolium methylsulphate) medium. The parameters of the oxidation reaction that have been investigated were the following: concentration of oxygen (5, 10, 15 and 20 ft3 h-1), reaction time (2, 4, 6, 8 and 10 h) and reaction temperature (25, 40, 60, 80 and ...
arxiv
Extracellular vesicles (EVs) play a dual role in diagnostics and therapeutics, offering innovative solutions for treating cancer, cardiovascular, neurodegenerative, and orthopedic diseases. This review highlights EVs’ potential to revolutionize personalized medicine through specific applications in disease detection and treatment.
Farbod Ebrahimi+4 more
wiley +1 more source
Rapid, quantitative separation by high-performance liquid chromatography of methylated bases in transfer RNA [PDF]
C.E. Salas, Otto Z. Sellinger
openalex +1 more source
Optimal Experimental Designs for Estimating Henry's Law Constants via the Method of Phase Ratio Variation [PDF]
When measuring Henry's Law constants ($k_H$) using the phase ratio method via headspace gas chromatography (GC), the value of $k_H$ of the compound under investigation is calculated from the ratio of the slope to the intercept of a linear regression of the the inverse GC response versus the ratio of gas to liquid volumes of a series of vials drawn from
arxiv