Results 41 to 50 of about 22,239,888 (283)

Determination of itraconazole and hydroxyitraconazole in plasma by use of liquid chromatography-tandem mass spectrometry with on-line solid-phase extraction [PDF]

, 2003
In this paper a method for the simultaneous quantification of the anti-fungal drug itraconazole and its co-active metabolite hydroxyitraconazole in plasma employing liquid chromatography tandem-mass spectrometry and automated solid-phase extraction is ...
Spöhrer, Ute, Schiel, Xaver, Vogeser, Michael   +2 more
core   +1 more source

Development and validation of RP-HPLC and RP-UPLC methods for quantification of erythropoietin formulated with human serum albumin

Journal of Pharmaceutical Analysis, 2012
Rapid and sensitive reverse phase high performance liquid chromatography (RP–HPLC) and ultra performance liquid chromatography (UPLC) methods with UV detection for quantification of erythropoietin (EPO) in presence of human serum albumin (HSA) as a ...
Shaligram S. Rane, Alkesh Ajameri, Rustom Mody, P. Padmaja   +3 more
doaj   +1 more source

Principles of High Performance Liquid Chromatography (HPLC)

, 2021
HPLC is one of the most common separation techniques in many sciences. This paper first refers to the history of chromatography, its terms and relationships, and then to the careful selection of instrumental and laboratory parameters in order to perform an efficient separation. Parameters such as the choice of column type and the choice of moving phase,
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Fractionation of fecal neutral steroids by high performance liquid chromatography.

Journal of Lipid Research, 1985
Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids.
E M Jackson, C A Kloss, S T Weintraub, G E Mott   +3 more
doaj   +1 more source

Sustainablity evaluation of existed HPLC based analytical methods for quantification of amlodipine besylate and telmisartan using RGB model: A whiteness approach

Green Analytical Chemistry, 2023
: For drug analysis, many spectroscopic techniques, including infrared, mass, NMR, and UV spectroscopy, as well as chromatographic techniques like ultra-performance liquid chromatography, thin-layer chromatography, high-pressure thin-layer chromatography,
Suvarna Yenduri, Huma Sulthana, Naga Prashant Koppuravuri   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Light-emitting diodes and photodiodes in the deep ultra-violet range for absorption photometry in liquid chromatography, capillary electrophoresis and gas sensing [PDF]

, 2016
Absorbance measurement in the deep ultra-violet range (below 300 nm) has been one of the most widely used detection methods for analytical techniques as a large number of organic compounds have strong absorption bands in the deep UV region.
Bui, Duy Anh
core   +1 more source

Quantitative analysis of monosialogangliosides by high-performance liquid chromatography of their perbenzoyl derivatives.

Journal of Lipid Research, 1979
A quantitative high-performance liquid chromatographic method for the analysis of monosialogangliosides as their perbenzoyl derivatives has been devised.
E G Bremer, S K Gross, R H McCluer
doaj   +1 more source

High-performance liquid chromatography (HPLC) assay for ribavirin and comparison of the HPLC assay with radioimmunoassay [PDF]

Antimicrobial Agents and Chemotherapy, 1989
The use of high-performance liquid chromatography (HPLC) to measure ribavirin in serum and other biological fluids has been limited by endogenous interfering substances. We report an HPLC procedure based on the extraction of ribavirin from serum, plasma, or cerebrospinal fluid with a boronate affinity gel, which uses a 3-methylcytidine internal ...
G G, Granich, D J, Krogstad, J D, Connor, K L, Desrochers, C, Sherwood   +4 more
openaire   +2 more sources