Results 61 to 70 of about 719,896 (409)
FEBS Open Bio, EarlyView.We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques +5 more
wiley +1 more source
Separation of the natural retinoids by high-pressure liquid chromatography
Journal of Lipid Research, 1978 A reverse phase high-pressure liquid chromatography system for rapid separation of various retinoids (vitamin A and its analogs) with little or no degradation is described. This method permits detection of as little as 22 pmol of retinoic acid.
C A Frolik, T E Tavela, M B Sporn
doaj
Truncated glucagon-like peptide I, an insulin-releasing hormone from the distal gut [PDF]
, 1987 By hydrophobic gel permeation and high pressure liquid chromatography we isolated from pig intestinal mucosa a peptide which corresponds to proglucagon 78–107 as suggested by chromatography and determination of its N-terminal sequence.
Holst, J.J. +3 more
core +1 more source
FEBS Open Bio, EarlyView.Osimertinib reduces angiogenesis and PDL1 expression in in ovo tumors, transforming them into ‘cold tumors’ with lower immune activity. Anatomopathological and transcriptomic analyses highlight its therapeutic impact on tumor biology. This study underscores osimertinib's potential to reshape the tumor microenvironment and provides insights into its ...
David Barthélémy +14 more
wiley +1 more source
American Journal of Clinical Pathology, 2008 The Sebia Capillarys (capillary zone electrophoresis [CE]) and the Primus Resolution high-pressure liquid chromatography (HPLC) were used to prospectively evaluate 297 samples for hemoglobinopathies.
D. Keren +4 more
semanticscholar +1 more source
Tropomyosin-like properties of clathrin light chains allow a rapid, high-yield purification. [PDF]
, 1983 The light chains (LCa and LCb) of bovine brain clathrin are resistant to heat denaturation by boiling, a property shared by tropomyosin (Bailey, K., 1948, Biochem. J., 43:271-281).
Brodsky, FM, Holmes, NJ, Parham, P
core +2 more sources
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACTObjectiveCerebrospinal fluid (CSF) orexin‐A has been suggested to be a biomarker of Alzheimer disease (AD). In both cognitively unimpaired healthy older adults and individuals with symptomatic AD, CSF orexin‐A is positively associated with CSF Aβ42, p‐tau181, and total tau (t‐tau) concentrations.
Ruijin Lu +13 more
wiley +1 more source
Complete gradient-LC-ESI system on a chip for protein analysis [PDF]
, 2005 This paper presents the first fully integrated gradient-elution liquid chromatography-electrospray ionization (LC-ESI) system on a chip. This chip integrates a pair of high-pressure gradient pumps, a sample injection pump, a passive mixer, a packed ...
Lee, T. D. +3 more
core
Advanced Biology, EarlyView.Extracellular vesicles (EVs) play a dual role in diagnostics and therapeutics, offering innovative solutions for treating cancer, cardiovascular, neurodegenerative, and orthopedic diseases. This review highlights EVs’ potential to revolutionize personalized medicine through specific applications in disease detection and treatment.
Farbod Ebrahimi +4 more
wiley +1 more source
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis.
Journal of Lipid Research, 1988 Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol.
K Prydz, B F Kase, J I Pedersen
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