Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Non-Contact State Assessment of Falling-Film Flow over Horizontal Tube Bundles Using High-Speed Imaging. [PDF]
Wang W, Tian M, Zhang G, Qiu Y.
europepmc +1 more source
Moxifloxacin based axially swept wide-field fluorescence microscopy for high-speed imaging of conjunctival goblet cells. [PDF]
Lee J, Kim S, Yoon CH, Kim MJ, Kim KH.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
High-speed imaging of CNT deagglomeration in aqueous solution with surfactant. [PDF]
Xu Z, Tonry C, Shaffer MSP, Li Q.
europepmc +1 more source
High-Speed Visual Imaging (HiViz) provides "information and inspiration especially for students, teachers and hobbyists." Their goal is to dispel common misconceptions about high-speed photography, in particular that it is only for experts and ...
core
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Multi-objective optimization of low-cost high-pressure glasshouse atomization: High-speed imaging mechanism for targeted droplet uniformity, environmental sustainability, and whitefly control. [PDF]
Yu Z +8 more
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
High-speed imaging and coumarin dosimetry of horn type ultrasonic reactors: Influence of probe diameter and amplitude. [PDF]
Viciconte G +4 more
europepmc +1 more source

