Results 131 to 140 of about 1,036,014 (384)

Use of high throughput sequencing to observe genome dynamics at a single cell level

, 2009
With the development of high throughput sequencing technology, it becomes possible to directly analyze mutation distribution in a genome-wide fashion, dissociating mutation rate measurements from the traditional underlying assumptions. Here, we sequenced
A. Soldatov, Bentley, Benzer, Bhanot, Cai, Cairns, Cox, Cupples, D. Parkhomchuk, Dotsch, Elowitz, Foster, Foster, Gabriel, Goodarzi, Hall, Hill-Perkins, Lindahl, Loechler, Loveless, Meselson, Mitra, Niki, Raser, Rosche, Sedgwick, Singer, V. Amstislavskiy, V. Ogryzko, Yu   +29 more
core   +1 more source

Microfluidic electro‐viscoelastic manipulation of extracellular vesicles

FEBS Open Bio, EarlyView.
The electro‐viscoelastic manipulation as a potential method for separation of particles based on size. The particles introduced as a sheath flow migrate to the channel center under the influence of simultaneously applied electric field and pressure driven flow.
Seyedamirhosein Abdorahimzadeh, Éva Bozó, Zikrullah Bölükkaya, Artem Zhyvolozhnyi, Anatoliy Samoylenko, Henrikki Liimatainen, Seppo J. Vainio, Caglar Elbuken   +7 more
wiley   +1 more source

Metagenomic Sequencing of Maize Reveals Abundant Genomic RNA of a Comovirus, a Genus Previously Known to Infect Only Dicots [PDF]

The Plant Pathology Journal
To better understand the diversity of viral pathogens in Türkiye, a major exporter of cereals in Europe, we performed high-throughput sequencing of total RNA from maize plants collected in the Trakya region.
Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W. Allen Miller   +4 more
doaj   +1 more source

Review of applications of high-throughput sequencing in personalized medicine: barriers and facilitators of future progress in research and clinical application

Briefings Bioinform., 2019
There has been an exponential growth in the performance and output of sequencing technologies (omics data) with full genome sequencing now producing gigabases of reads on a daily basis. These data may hold the promise of personalized medicine, leading to
Gaye Lightbody, Valeriia Haberland, Fiona Browne, Laura Taggart, Huiru Zheng, E. Parkes, J. Blayney   +6 more
semanticscholar   +1 more source

The Principles and Applications of High-Throughput Sequencing Technologies

Development & Reproduction, 2023
The advancement in high-throughput sequencing (HTS) technology has revolutionized the field of biology, including genomics, epigenomics, transcriptomics, and metagenomics. This technology has become a crucial tool in many areas of research, allowing scientists to generate vast amounts of genetic data at a much faster pace than traditional methods. With
openaire   +2 more sources

FLASH: ultra-fast protocol to identify RNA-protein interactions in cells

, 2019
Determination of the in vivo binding sites of RNA-binding proteins (RBPs) is paramount to understanding their function and how they affect different aspects of gene regulation.
Akhtar, A., Aktas, T., Backofen, R., Ilik, I., Maticzka, D.   +4 more
core   +1 more source

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source

A Reference Viral Database (RVDB) To Enhance Bioinformatics Analysis of High-Throughput Sequencing for Novel Virus Detection

Msphere, 2018
To facilitate bioinformatics analysis of high-throughput sequencing (HTS) data for the detection of both known and novel viruses, we have developed a new reference viral database (RVDB) that provides a broad representation of different virus species from
Norman Goodacre, Aisha AlJanahi, S. Nandakumar, Mike Mikailov, Arifa S. Khan   +4 more
semanticscholar   +1 more source

Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. [PDF]

, 2018
High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with ...
Byrne, Ashley, Cole, Charles, Green, Richard E, Palmer, Theron, Schmitz, Robert J, Volden, Roger, Vollmers, Christopher   +6 more
core   +1 more source

A unified model for Duchenne muscular dystrophy gene involvement in cancer: context‐dependent tumour suppression and oncogenicity

FEBS Open Bio, EarlyView.
We propose a context‐dependent model where the Duchenne muscular dystrophy (DMD) gene acts as a tumour suppressor in aggressive tumours and as an oncogene in less aggressive ones. We propose this model as a unified framework to explain the opposing survival associations with DMD expression and to guide experimental exploration of the dual role of DMD ...
Lee Machado, Leanne Jones, Sonika Divakar, Michael Naidoo, Karen Anthony   +4 more
wiley   +1 more source