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DNA sequencing in high-throughput neuroanatomy
Journal of Chemical Neuroanatomy, 2019Mapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are often very labor intensive and do not scale well to the complexity of mammalian brains.
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Analysis of High-Throughput Sequencing Data
2010Next-generation sequencing has revolutionized biology by exponentially increasing sequencing output while dramatically lowering costs. High-throughput sequence data with shorter reads has opened up new applications such as whole genome resequencing, indel and SNP detection, transcriptome sequencing, etc.
Shrinivasrao P, Mane +2 more
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High-Throughput HIV Sequencing: Evolution in 2D
Future Virology, 2011Evaluation of: Willerth SM, Pedro HA, Pachter L, Humeau LM, Arkin AP, Schaffer DV: Development of a low bias method for characterizing viral populations using next generation sequencing technology. PLoS ONE 5(10), E13564 (2010). The recent commercialization of high-throughput sequencing technologies has brought about the possibility of characterizing ...
Vrancken, Bram, Lemey, Philippe
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Nonoverlapping Clone Pooling for High-Throughput Sequencing
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2012Simultaneously sequencing multiple clones using second-generation sequencers can speed up many essential clone-based sequencing methods. However, in applications such as fosmid clone sequencing and full-length cDNA sequencing, it is important to create pools of clones that do not overlap on the genome for the identification of structural variations and
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High-throughput single-cell RNA sequencing
Trends in Plant Science, 2022Tom Denyer, Marja C.P. Timmermans
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High-Throughput Sequencing and Metagenomics
Estuaries and Coasts, 2009The fields of biology and environmental health are undergoing an unprecedented transformation in the way communities are characterized and studied. One of the catalysts for this change has been the advent of high-throughput sequencing (aka next-generation sequencing).
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High-Throughput Sequencing of Complete Mitochondrial Genomes
2016Next-generation sequencing has revolutionized mitogenomics, turning a cottage industry into a high throughput process. This chapter outlines methodologies used to sequence, assemble, and annotate mitogenomes of non-model organisms using Illumina sequencing technology, utilizing either long-range PCR amplicons or gDNA as starting template.
Andrew George, Briscoe +2 more
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มะเร็งปากมดลูกมีสาเหตุหลักมาจากการติดเชื้อไวรัสฮิวแมนปาปิลโลมาในกลุ่ม high risk genotype แต่สามารถพบการติดเชื้อในกลุ่ม low risk genotype ในผู้ป่วยมะเร็งปากมดลูกด้วย ซึ่งเทคนิคที่ใช้ในปัจจุบันไม่สามารถตรวจพบได้ ในขณะที่เทคโนโลยีที่มีประสิทธิภาพสูงอย่าง high-throughput sequencing สามารถตรวจพบสายพันธุ์ของไวรัสได้คลอบคลุมมากกว่าแต่มีข้อเสียในเรื่องค่าใช้จ ...
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Bioinformatics for High Throughput Sequencing
2012Preface.- Introduction.- Overview of Sequencing Technology Platforms.- Applications of High Throughput Sequencing.- Computational Infrastructure and Basic Data Analysis for High-Throughput Sequencing.- Base-Calling for Bioinformaticians.- De Novo Short Read Assembly.- Short Read Mapping.- DNA-Protein Interaction Analysis (ChIP-Seq).- Genome-wide ...
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