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QTL Mapping Using High-Throughput Sequencing
2015Quantitative trait locus (QTL) mapping in plants dates to the 1980s (Stuber et al. Crop Sci 27: 639-648, 1987; Paterson et al. Nature 335: 721-726, 1988), but earlier studies were often hindered by the expense and time required to identify large numbers of polymorphic genetic markers that differentiated the parental genotypes and then to genotype them ...
Tiffany M, Jamann +2 more
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DNA sequencing in high-throughput neuroanatomy
Journal of Chemical Neuroanatomy, 2019Mapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are often very labor intensive and do not scale well to the complexity of mammalian brains.
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Analysis of High-Throughput Sequencing Data
2010Next-generation sequencing has revolutionized biology by exponentially increasing sequencing output while dramatically lowering costs. High-throughput sequence data with shorter reads has opened up new applications such as whole genome resequencing, indel and SNP detection, transcriptome sequencing, etc.
Shrinivasrao P, Mane +2 more
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Promises and pitfalls of using high‐throughput sequencing for diet analysis
Molecular Ecology Resources, 2018The application of high‐throughput sequencing‐based approaches to DNA extracted from environmental samples such as gut contents and faeces has become a popular tool for studying dietary habits of animals.
A. Alberdi +6 more
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High-Throughput HIV Sequencing: Evolution in 2D
Future Virology, 2011Evaluation of: Willerth SM, Pedro HA, Pachter L, Humeau LM, Arkin AP, Schaffer DV: Development of a low bias method for characterizing viral populations using next generation sequencing technology. PLoS ONE 5(10), E13564 (2010). The recent commercialization of high-throughput sequencing technologies has brought about the possibility of characterizing ...
Vrancken, Bram, Lemey, Philippe
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Nonoverlapping Clone Pooling for High-Throughput Sequencing
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2012Simultaneously sequencing multiple clones using second-generation sequencers can speed up many essential clone-based sequencing methods. However, in applications such as fosmid clone sequencing and full-length cDNA sequencing, it is important to create pools of clones that do not overlap on the genome for the identification of structural variations and
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High-throughput sequencing view on the magnitude of global fungal diversity
Fungal diversity, 2021P. Baldrian +3 more
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High-throughput single-cell RNA sequencing
Trends in Plant Science, 2022Tom Denyer, Marja C.P. Timmermans
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High-Throughput Sequencing and Metagenomics
Estuaries and Coasts, 2009The fields of biology and environmental health are undergoing an unprecedented transformation in the way communities are characterized and studied. One of the catalysts for this change has been the advent of high-throughput sequencing (aka next-generation sequencing).
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High-Throughput Sequencing of Complete Mitochondrial Genomes
2016Next-generation sequencing has revolutionized mitogenomics, turning a cottage industry into a high throughput process. This chapter outlines methodologies used to sequence, assemble, and annotate mitogenomes of non-model organisms using Illumina sequencing technology, utilizing either long-range PCR amplicons or gDNA as starting template.
Andrew George, Briscoe +2 more
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