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High Performance Liquid Chromatography
2019High performance liquid chromatography (HPLC) is a form of liquid chromatography which is used to separate the individual components of interest present in mixture and/or dissolved in sample solution. It is based on pumping of mobile phase through the packed column under high pressure.
Muhammad Sajid Hamid Akash+1 more
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High-performance liquid chromatography of proteins
Journal of Chromatography A, 1976Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase ...
Karen M. Gooding+2 more
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High-performance liquid chromatography of antibiotics
Journal of Chromatography B: Biomedical Sciences and Applications, 1990High-performance liquid chromatographic (HPLC) monitoring of antimicrobial agents has recently become more widely used, and represents an interesting alternative to other methods. The methodology is characterized by good specificity and accuracy, and it is applicable to almost all antibiotics.
François Jehl+2 more
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High performance liquid chromatography
1999The pioneering work of Martin and Synge [1] led to the development of gas-liquid chromatography, an elegant chromatographic method where, even with packed columns, the efficiencies achieved were high and the technique could be used with equal facility for both analytical and preparative studies.
F. J. Smith, A. Braithwaite
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High-performance liquid magneto-chromatography
Journal of Chromatography A, 2006High-performance liquid magneto-chromatography (HPLMC) is a new chromatography technique with two distinctive features: (a) a high surface area stationary phase with paramagnetic properties composed of magnetite embedded in a silica gel, and (b) a magnetic field (variable intensity 0-5.5 mT) that selectively retains paramagnetic substances in the ...
Jose A. Rodriguez, Enrique Barrado
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High-performance liquid chromatography of tetracyclines
Journal of Chromatography A, 1986The possibilities of two non-ionogenic resins (XAD-2 and PRP-1) in the chromatography of tetracyclines and their degradation products are described. It appears that, in contrast to silica-based materials, this type of column material produces linear calibration curves, even in the low nanogram range.
U.R. Tjaden, H.J.E.M. Reeuwijk
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High-Performance Liquid Chromatography
1982High-performance liquid chromatography (HPLC) has become a standard technique in the laboratory. The intent of this chapter is to present an overview of the technical aspects of HPLC rather than an in-depth discussion. For more detailed information on HPLC, the reader is referred to the recently revised text by Synder and Kirkland.1 There is an ...
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Measurement of food flavonoids by high-performance liquid chromatography: A review.
Journal of Agricultural and Food Chemistry, 2000The flavonoids are plant polyphenols found frequently in fruits, vegetables, and grains. Divided into several subclasses, they include the anthocyanidins, pigments chiefly responsible for the red and blue colors in fruits, fruit juices, wines, and ...
H. Merken, G. Beecher
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High-Performance Liquid Chromatography of Cisplatin
Journal of Pharmaceutical Sciences, 1983The retention behavior of cisplatin on a variety of stationary phases has been investigated using aqueous mobile phases modified by the addition of various electrolytes and methanol. Cisplatin is poorly retained on reverse-phase or silica columns but satisfactorily retained on chemically bonded or solvent-generated anion exchangers.
A.J. Repta+2 more
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