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A Review on High Performance Liquid Chromatography (HPLC)
Asian Journal of Pharmaceutical Analysis, 2023The estimate of pharmaceutical and biological materials typically uses the key qualitative and quantitative technique known as high performance liquid chromatography (HPLC). It is the most adaptable, secure, dependable, and quick chromatographic method for determining the quality of medicinal ingredients.
Sahil Kamble +5 more
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High Performance Liquid Chromatography (HPLC)
1985Liquid chromatography (LC) is the generic name used to describe any chromatographic procedure in which the mobile phase is a liquid. Techniques already discussed which belong to this general classification are paper chromatography, thin-layer chromatography (TLC) and variants of traditional column chromatography.
A. Braithwaite, F. J. Smith
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Separation of Hemoglobin Peptides by high Performance Liquid Chromatography (HPLC)
Hemoglobin, 1980(1980). Separation of Hemoglobin Peptides by high Performance Liquid Chromatography (HPLC) Hemoglobin: Vol. 4, No. 3-4, pp. 551-559.
W A, Xchroeder +2 more
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Determination of hexamethylenetetramine in foods by high-performance liquid chromatography (HPLC)
Food Additives & Contaminants: Part A, 2014A simple and rapid method was developed and validated for the determination of hexamethylenetetramine (HMT) in foods. Samples were homogenised and extracted with methanol, followed by centrifugation. The resulting solution was filtered and injected into the high-performance liquid chromatograph (HPLC).
HoSoo, Lim +3 more
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High performance liquid chromatography (HPLC) in the investigation of gout in palaeopathology
International Journal of Osteoarchaeology, 2008AbstractGout is a disease caused by the abnormal accumulation of uric acid in the body, which can result in sodium urate crystals forming tophi at joints, with associated erosion of bone and cartilage. Only two examples of tophi have been reported from archaeological individuals, and the diagnosis of gout based on dry bone manifestations can be ...
Swinson, D.J. +3 more
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High Performance Liquid Chromatography (HPLC)
1992The general principles of liquid chromatography were first outlined in 1903 by the botanist Tswett in his description of adsorption chromatography for the separation of natural pigments, such as chlorophylls (Tswett 1903). Since then remarkable progress has been made, as evidenced by the multitude of techniques employed today in thin layer, low ...
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Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)
2014The size exclusion-high-performance liquid chromatography is a high-throughput analytical method, through isocratic condition, that allows to determine and quantify the level of aggregates and fragments of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, quantity injected,
Delphine, Schrag +2 more
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Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)
2014The ion exchange-high-performance liquid chromatography is a high-throughput analytical method that allows to determine the charge profile of purified antibodies. Here, we describe the preparation of the samples, chromatographic conditions to be used (buffer preparation, salt gradient, quantity injected, flow rate, run time, column suitability, etc ...
Marie, Corbier +2 more
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Purification of Monoclonal Antibodies Using High Performance Liquid Chromatography (HPLC)
Preparative Biochemistry, 1984Recent increases in the ability to detect low levels of immunofluorescence have shown the need for highly purified primary immunoreagents. There are now reports of purification of monoclonal antibodies using HPLC with reverse phase columns. In this study we have utilized standard size exclusion HPLC to purify both biotinylated and non-biotinylated ...
G A, Perry +4 more
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Chiral High-Performance Liquid Chromatography (HPLC) in Targeted Metabolomics
Enzymatic reactions usually occur with a high substrate, product, or substrate-product stereoselectivity. In many instances, information on the stereochemical disposition of biologically active metabolites is indispensable for a complete understanding of biological processes and metabolic states.Xiaoqing, Fu, Michael, Lämmerhofer
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