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High Performance Thin-Layer Chromatography

2013
The current chapter emphasizes the use of high-performance thin-layer chromatography (HPTLC) as a green analytical separation alternative. The chapter provides historical development of thin-layer chromatography towards becoming modern, automated, high resolution technique in the form of high-performance thin-layer chromatography, and their further ...
Milojković-Opsenica, Dušanka   +1 more
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Pressurized planar electrochromatography, high-performance thin-layer chromatography and high-performance liquid chromatography—Comparison of performance

Journal of Chromatography A, 2010
Kinetic performance, measured by plate height, of High-Performance Thin-Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Pressurized Planar Electrochromatography (PPEC) was compared for the systems with adsorbent of the HPTLC RP18W plate from Merck as the stationary phase and the mobile phase composed of acetonitrile and ...
Paweł, Płocharz   +2 more
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High-Performance Thin Layer Chromatography Instrumentation

2023
HPTLC is one type of planar chromatography and the most advanced form of instrumental TLC. HPTLC equipment involves an autosampler for the application of a specific amount of sample on the plate, after spotting of sample the plate is developed in a TLC development chamber after development the plate is dried and visualized using visualizer.
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Quantitative high-performance thin-layer chromatography of nucleosides

Russian Journal of Applied Chemistry, 2010
A procedure was developed for express analysis of thymidine by high-performance thin-layer chromatography in the cultural liquid in the course of microbiological synthesis.
I. I. Malakhova   +3 more
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Anticircular high performance thin‐layer chromatography

Journal of High Resolution Chromatography, 1978
AbstractThe principal analytical details for the third of three possible modes in high performance thin‐layer chromatography are given, namely the anticircular mode. Separation is achieved by allowing the mobile phase to enter the plate layer on a precise outer circle line, from where it flows towards the centre with nearly constant speed.
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Stationary phases for high-performance thin-layer chromatography

Journal of Chromatography A, 1980
Abstract The characteristics of commercially available high-performance thin-layer chromatographic quality thin-layer plates pre-coated with silica gel, cellulose, chemically bonded phases or polyamide are discussed and compared with those of pre-coated layers used in conventional thin-layer chromatography.
U.A. Th. Brinkman   +2 more
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Quantitation in high-performance micro-thin-layer chromatography

Journal of Chromatography A, 1975
Abstract Silica gel 60 F254 pre-coated plates for nanogram-scale thin-layer chromatography were developed for high-performance thin-layer chromatography (HPTLC) on the basis of silica gel of pore size 60 A. Under the usual chromatographic conditions, it was possible to achieve a plate height of 12 μm.
J. Ripphahn, H. Halpaap
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Introduction to High-Performance Thin Layer Chromatography (HPTLC)

2023
This chapter discusses chromatography, classification of chromatography, thin layer chromatography (TLC) and how high-performance thin-layer chromatography (HPTLC) is an advanced version of TLC. In brief, this chapter also discusses the advantages and the areas where the HPTLC technique can be used.
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Determination of cortisol in guinea pig urine by high performance thin-layer chromatography, high performance liquid chromatography, and thin-layer chromatography/radioimmunoassay

Chromatographia, 1997
In order to collect urinary samples from unrestrained guinea pigs, animals were kept in their familiar home cages with wood shavings for bedding. Cortisol was removed from shavings by a simple washing step, and an attempt was made to measure its concentrations by high performance thin-layer chromatography (HPTLC), high performance liquid chromatography
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Rapid microanalysis of anticonvulsants by high-performance thin-layer chromatography

Journal of Chromatography B: Biomedical Sciences and Applications, 1981
A rapid microanalytical method is described for phenobarbital, phenytoin, primidone and carbamazepine utilizing high-performance thin-layer chromatography (HPTLC). This procedure incorporates a single extraction of a 50-microliter plasma sample.
C M, Davis, D C, Fenimore
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