Results 201 to 210 of about 1,123,025 (269)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Development and testing of a high-precision static calibration device for aviation thin-film thermocouples. [PDF]

Sci Rep
Yang J, Fang C, Xu Z, Zhou C, Zhang K.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Deep-learning deconvolution and segmentation of fluorescent membranes for high-precision bacterial cell-size profiling. [PDF]

Commun Biol
Reyes-Matte O, Fortmann-Grote C, Gericke B, Hüttmann N, Ojkic N, López-Garrido J.   +5 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

High Precision Antibody-Free Microtubule Labeling for Expansion Microscopy. [PDF]

Bio Protoc
Chowdhury R, Krah D, Ntolkeras A, Heimbrodt A, Shaib AH.   +4 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Enable preclinical, high-precision image-guided radiotherapy research with a stand-alone small animal IGRT system. [PDF]

Med Phys
Deng W, Han D, Byrne KE, Bhandary B, Jiang K, Poirier YP, Mossahebi S, Xu J, Shukla H, Sawant A.   +9 more
europepmc   +1 more source

High-Precision Centroid Measurement Method Based on 3D Scanning and Hooke's Law. [PDF]

Sensors (Basel)
He X, Li Z, Pan X, Yang Y.
europepmc   +1 more source

High-precision microtest

Materials Today, 2001
openaire   +1 more source