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High pressure liquid chromatography of oligodeoxyribonucleotides
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973Abstract A high pressure liquid chromatography system capable of resolving oligodeoxy-ribonucleotides of intermediate size is described. Columns filled with a pellicular weak anion exchanger consisting of a polymeric aliphatic amine permit the rapid resolution of small amounts of oligomers.
A. de Czekala +6 more
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High-pressure liquid chromatography of peptides
Journal of Chromatography A, 1977Peptides varying in size from di- to decapeptide have been subjected to high-pressure liquid chromatography on Phenyl-Corasil, Poragel PN, and Poragel PS under reversed-phase conditions with acetonitrile-water mixtures. It has been found that residual silanol groups in the Phenyl-Corasil and the functional groups in the Poragels significantly influence
K. Nils-Gunnarjohansson +4 more
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High-pressured liquid chromatography of carotenoids
Journal of Chromatography A, 1978Abstract The potential of high-pressure liquid chromatography (HPLC) for separation of carotenoids on silica has been studied with selected model carotenoids (carotenes, diols, cis—trans isomers and diastereoisomers) in comparison with conventional thin-layer chromatography and circular paper techniques.
Anne Fiksdahl +2 more
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High Pressure Liquid Chromatography of Anthocyanins
Canadian Institute of Food Science and Technology Journal, 1975Abstract An investigation of several high pressure liquid chromatographic (hplc) systems, potentially useful in rapidly separating mixtures of anthocyanins, indicated that a polyamide material called Pellidon performed the best. Three monoglucosides of malvidin, petunidin and peonidin were successfully separated in less than 20 minutes on a ⅛″ × 4 ...
C.H. Manley, P. Shubiak
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High-pressure liquid chromatography of carbohydrates
Journal of Chromatography A, 1974Abstract A procedure is described for the separation of water-soluble wood polysaccharides on Bio-Glas (granular porous glass) and Bio-Gel P (polyacrylamide) packed columns using high-pressure liquid chromatography (HPLC). An example of HPLC employing non-aqueous solvent systems and EM Gel OR-PVA (vinyl acetate copolymer) type packing materials is ...
Gary D. McGinnis, Greg P. Belue
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High-pressure liquid chromatography on triacetylcellulose
Journal of Chromatography A, 1983Abstract Microcrystalline triacetylcellulose ( d p = 10–20 μm) was used for analytical liquid chromatography at pressures around 50 bar. 1,3,5-Tri- tert .-butylbenzene is proposed as a compound which is not retained on this sorbent. Thus, reliable information about porosity, linear flow-rates, u , and relative retentions become available for the ...
Karl-Heinz Rimböck +2 more
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Laboratory Automation of High‐Pressure Liquid Chromatography
Journal of Pharmaceutical Sciences, 1975An automated system for high-pressure liquid chromatography was developed. The system is built around commercial modules wherever possible, modified to varying degrees. An automatic sampler, a sample pump, a high-pressure sampling valve, a recorder with an integrator, and a high-pressure liquid chromatograph comprise the commercial instruments. Relays,
David D. Gleason, William F. Beyer
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Thimerosal Determination by High-Pressure Liquid Chromatography
Journal of Pharmaceutical Sciences, 1978A sensitive and useful high-pressure liquid chromatographic method for the determination of intact thimerosal was developed. This method is extremely fast and reliable, and its inherent specificity makes it a breakthrough over other common wet chemical methods.
Roger Carey Meyer, Lawrence B. Cohn
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Analysis of leukotrienes by high-pressure liquid chromatography
Analytical Biochemistry, 1981Abstract A reversed-phase high-pressure liquid chromatographic procedure has been developed to separate and quantitate leukotrienes. This procedure is based on the ultraviolet absorption of the conjugated triene moiety of these compounds and is sensitive to the nanogram level.
Robert C. Murphy +2 more
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Determination of hydroxyproline by high pressure liquid chromatography
Analytical Biochemistry, 1992A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated from other amino acids and detected by absorption at 254
Kathleen Reagan, Graham D. Green
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