Results 91 to 100 of about 8,231 (239)
Engineered Optogenetic Circuits In Yeast with Self‐Sustained Outputs
Advanced Science, EarlyView.Optogenetic quorum‐sensing (OptoQS) circuit enables Saccharomyces cerevisiae to record transient light stimulus with self‐sustained outputs. Panel a indicates the design of the OptoQS circuit based on G‐protein coupled receptor (GPCR) signaling cascade. Panel b indicates the mode of action of surrogate messenger‐mediated signal transmission at the cell
Cong Fan +7 more
wiley +1 more source
Femtosecond‐Laser‐Induced Physical Unclonable Random Maze Structure for Storage‐Free Encryption
Advanced Science, EarlyView.Femtosecond‐laser‐induced gold random maze structures serve as multimodal physical unclonable functions for storage‐free encryption. Their stochastic optical, electrical, and Raman responses are generated by plasmon‐assisted Marangoni formation and converted into AES‐compatible keys without permanent secret‐key storage, offering a portable route toward
Shiru Jiang +6 more
wiley +1 more source
On the Hill estimator: a comparison of methods
, 2015 Extreme value theory (EVT) deals with the occurrence of extreme phenomena. The tail index is a very important parameter appearing in the estimation of the probability of rare events. Under a semiparametric framework, inference requires the choice of a number k of upper order statistics to be considered.
Ferreira, Marta Susana +1 more
openaire +1 more source
FOS3D: A Fluorescence‐Enabled Toolkit for Characterizing a Three‐dimensional Osteosarcoma Model
Advanced Science, EarlyView.FOS3D describes fluorescent (F) osteosarcoma (OS) cells in a tri‐dimensional (3D) model. The study comprises three phases: development, where biofabrication parameters are tuned to achieve cytocompatibility and tumor‐specific mechanical properties in cell‐laden gelatin methacryloyl constructs; validation, where whole‐well fluorescence reading is ...
William Humble +9 more
wiley +1 more source
Versatile Detection of Cellular Protein via Fluorescence Anisotropy
Advanced Science, EarlyView.Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang +12 more
wiley +1 more source
Advanced Science, EarlyView.Both SVF and CD31+ SVF improve spontaneous osteoarthritis, but CD31+ enrichment refines the therapeutic profile rather than broadly enhancing all effects. More distinct benefits are observed in extracellular matrix remodeling, osteochondral integrity, and subchondral/trabecular bone preservation, supporting CD31+‐enriched SVF as a biologically enhanced
Sijia Zhou +16 more
wiley +1 more source
Spectrum estimates of Hill's lunar problem
, 2015 We investigate the action spectrum of Hill's lunar problem by observing inclusions between the Liouville domains enclosed by the regularized energy hypersurfaces of the rotating Kepler problem and Hill's lunar problem. In this paper, we reinterpret the spectral invariant corresponding to every nonzero homology class $α\in H_*(ΛN)$ in the loop homology ...
openaire +2 more sources
Advanced Science, EarlyView.This study shows that in glioma stem cells (GSCs), RBM12 recruits ALKBH5 to remove m6A from SLC7A5 transcripts, thereby enhancing mRNA stability, which elevates large neutral amino acid (LNAA) levels and activates mTORC1, promoting GSC proliferation, self‐renewal, and tumor growth.
Hong Lei +18 more
wiley +1 more source
Advanced Science, EarlyView.We have shown that both simulated and actual spaceflight contribute to cartilage degradation in the knees of mice. Using RNA sequencing and bioenergetic profiling, we identified NADPH oxidase 4 (NOX4) as a key factor driving these changes. Additionally, we found that kaempferol, a naturally occurring flavonoid that binds directly to NOX4, reduces ...
Yuesong Yin +23 more
wiley +1 more source
Advanced Science, EarlyView.scTIDE identifies single‐cell tipping points by combining manifold‐based graph representations with optimal‐transport conditional flow matching, which preserves intrinsic topology and models distributional dynamics. It supports critical‐transition detection at individual‐cell resolution, prediction of unseen cells, and dimensionality reduction and ...
Jiayuan Zhong +6 more
wiley +1 more source