Results 21 to 30 of about 112,497 (212)

Analiza genskega zapisa acidocinov LF221 A in B seva Lactobacillus gasseri LF221

Acta Agriculturae Slovenica, 2003
Z metodami molekularne biologije smo pripravili odseka DNA seva LF221 z zapisom za acidocin LF221 A oziroma acidocin LF221B. Na 0,9 kb dolgem odseku BamHI/HindIII, ki ima zapis za acidocin LF221 A, smo ugotovili prisotnost dveh bralnih okvirjev (ORF) ter
Andreja ČANŽEK MAJHENIČ, Irena ROGELJ
doaj   +1 more source

Association Study between BGLAP Gene HindIII Polymorphism and Type 2 Diabetes Mellitus Development in Ukrainian Population

Journal of Diabetes Research, 2019
Type 2 diabetes mellitus (T2DM) belongs to the diseases with hereditary predisposition, so both environmental and genetic factors contribute to its development.
Yaroslav D. Chumachenko, Viktoriia Yu. Harbuzova, Alexander V. Ataman   +2 more
doaj   +1 more source

Diferenciación de especies de Rhodococcus mediante una prueba de PCR-RFLP basada en los genes codificantes para la subunidad 16S ribosomal

NOVA, 2005
Dada la dificultad en diferenciar las especies de Rhodococcus por pruebas bioquímicas, se desarrolló una prueba de Reacción en Cadena de la Polimerasa (PCR), seguida de un ensayo de Polimorfismo de Longitud de Fragmentos de Restricción (PCR-RFLP) para la
Paula Pavía, Camila Calderon, Concepción Puerta   +2 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

A moderately frequent HindIII polymorphism at the human NGFR locus (17q12 – 17q22) [PDF]

Nucleic Acids Research, 1989
The clone Lambda6 contains human NGFR sequences cloned in the vector CH28. HindIII (AAGCTT) reveals allelic bands of 13 kb and 8.3+4.7 kb with a constant band of 8.8 kb. The NGFR locus has been assigned to 17q12->17q22 with somatic cell hybrid analysis and in situ hybridization.
E C, Wright, P R, Fain, D F, Barker, M V, Chao   +3 more
openaire   +2 more sources

Assembling a True “Olympic Gel” From over 16 000 Combinatorial DNA Rings

Advanced Materials, EarlyView.
Olympic gels are an elusive class of soft matter, consisting of molecular networks held together purely by mechanically interlocked rings. Their topological structure promises unique properties and functions, but their synthesis has proven notoriously difficult.
Sarah K. Speed, Yu‐Hsuan Peng, Azra Atabay, Krishna Gupta, Toni Müller, Carolin Fischer, Ilka M. Hermes, Jens‐Uwe Sommer, Michael Lang, Elisha Krieg   +9 more
wiley   +1 more source

Microfluidic Hydroporation Platform for Effective Allogeneic CAR‐T Cell Production and Subsequent Functional Cytotoxicity Analysis

Advanced Materials Technologies, EarlyView.
The hydroporator platform employs controlled hydrodynamic deformation for efficient mRNA and CRISPR/Cas9 delivery into primary human T cells, enabling allogeneic CAR‐T cell manufacturing. It preserves cell functionality and drives potent gene editing, CAR expression, and tumor cytotoxicity, while feature‐based analysis links these functional outcomes ...
Soohyun Jeon, Sein Kim, Hyun‐Young Kim, Duck Cho, Sungsu Park, Seohyun Kim, Aram J. Chung   +6 more
wiley   +1 more source

Super‐Enhancer‐Driven SOX4/SMAD3 Mediate Membrane Remodeling by Regulating Phospholipid Metabolism to Accelerate Leukemia Progression

Advanced Science, EarlyView.
Chronic myeloid leukemia blast phase (CML‐BP) poses a severe therapeutic challenge. This study reveals that the super‐enhancer‐driven transcription factors SOX4 and SMAD3 form a cooperative axis critical for disease progression. They co‐activate the oncogenic kinase AXL and promote phospholipid remodeling via LPCAT1 to facilitate its signaling ...
Enzhe Lou, Peilong Lai, Guanjie Peng, Bo Lu, Lizhen Jiang, Xinyue Li, Jinghong Chen, Ladi Mo, Qiong Mao, Haichuan Zhang, Jinxin Fang, Yi Meng, Aochu Liu, Yingyin Gao, Andong Huang, Wenhui Lin, Shiwen Hu, Zerong Guan, Xiaolan Ye, Zhenguo Liu, Liling Jiang, Yueyuan Zheng, Xianping Shi   +22 more
wiley   +1 more source

The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis

Advanced Science, EarlyView.
SLC39A13/ZIP13, a newly discovered intracellular iron transporter, delivers iron to the ER/Golgi to catalyze procollagen hydroxylation during collagen maturation. Here, we systematically characterize the cell type‐specific functions of ZIP13 across distinct hepatic cell populations, and identify hepatic stellate cell‐specific ZIP13 as a promising and ...
Shanshan Guo, Yalin Wang, Binyu Lu, Yu Zhang, David M. Frazer, Bing Zhou   +5 more
wiley   +1 more source