Results 1 to 10 of about 1,381,507 (269)

PCR-RFLP analysis of rbcL chloroplast gene [PDF]

open access: yesBIO Web of Conferences, 2022
To identify intergeneric, interspecific, and intraspecific polymorphisms, PCR-RFLP studies of the chloroplast rbcL gene profiles were carried out using five restriction endonucleases (AluI, HpaII, BsuRI, BstHHI, BstMBI) in 14 species belonging to 6 ...
Sheikina Olga
doaj   +2 more sources

Molecular authentication of Maytenus sp by PCR-RFLP
Autenticação molecular de Maytenus sp por PCR-RFLP

open access: yesSemina: Ciências Agrárias, 2013
Maytenus aquifolia and Maytenus ilicifolia are native plants from South America and popularly known as ‘Espinheira-santa’. Both are used as tea due to their efficiency in the treatment of ulcer, gastritis and indigestion.
Sandra Sayuri Nakamura   +4 more
doaj   +2 more sources

Molecular Typing of Methicillin Resistant Staphylococcus aureus using coa Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism: A Cross-sectional Study [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2022
Introduction: Methicillin Resistant Staphylococcus aureus (MRSA) are the most important multidrug resistant pathogen of humans causing a wide array of infections.
P Hema, B Appalaraju, R Someshwaran
doaj   +1 more source

تمرکز ایزوالکتریک و روشهای متصل به PCR-RFLP برای کشف کمبود Alpha1-antitrypsin [PDF]

open access: yesArchives of Razi Institute, 2004
53 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF ...
ب.غ. گودرزی   +4 more
doaj   +1 more source

PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil

open access: yesElectronic Journal of Biotechnology, 2015
Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses.
Diego Rayan Teixeira de Sousa   +8 more
doaj   +1 more source

Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP

open access: yesJournal of Pharmacopuncture, 2009
Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences ...
Ki-Rok Kwon, Seung-Il Baek, Suk Ho Choi
doaj   +1 more source

Typing class I HLA-A gene using a nested PCR-RFLP procedure

open access: yesBrazilian Journal of Medical and Biological Research, 2005
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution ...
E.C. Castelli   +3 more
doaj   +1 more source

Animal Species Identification by PCR – RFLP of Cytochrome b

open access: yesScientific Papers Animal Science and Biotechnologies, 2023
An alternative DNA detection system is based on the polymerase chain reaction (PCR) amplification of a segment of the mitochondrial cytochrome b gene.
Tomáš Minarovič   +3 more
doaj  

Métodos subsidiários para o diagnóstico da Leishmaniose tegumentar americana (LTA): comparação dos resultados do seqüenciamento de DNA e da PCR-RFLP para determinação da espécie de leishmania em amostras cutâneo-mucosas Subsidiary methods for the diagnosis of American tegumentar leishmaniasis (ATL): comparison of sequencing of DNA and PCR-RFLP for identification of leishmania species in skin samples

open access: yesAnais Brasileiros de Dermatologia, 2005
FUNDAMENTOS: Métodos moleculares têm-se mostrados mais eficazes para o diagnóstico da LTA. OBJETIVOS: Comparar os resultados da intradermorreação de Montenegro (IRM), presença de leishmania em biópsia (Bc), reação de imunofluorescência indireta (Rifi ...
Flávio C. Barbosa Garcia   +4 more
doaj   +1 more source

Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages

open access: yesCzech Journal of Food Sciences, 2017
The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in
Ivona Djurkin Kušec   +5 more
doaj   +1 more source

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