Results 11 to 20 of about 43,727 (186)

PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil

Electronic Journal of Biotechnology, 2015
Background: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses.
Diego Rayan Teixeira de Sousa, Carla Silvana da Silva Santos, Bodo Wanke, Roberto Moreira da Silva Júnior, Marcelo Cordeiro dos Santos, Kátia Santana Cruz, Rossicléia Lins Monte, Andreas Nocker, João Vicente Braga de Souza   +8 more
doaj   +1 more source

Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP

Journal of Pharmacopuncture, 2009
Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences ...
Ki-Rok Kwon, Seung-Il Baek, Suk Ho Choi
doaj   +1 more source

Typing class I HLA-A gene using a nested PCR-RFLP procedure

Brazilian Journal of Medical and Biological Research, 2005
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution ...
E.C. Castelli, D.S. Gil, L.C.S. Veiga, J.L.V. de Camargo   +3 more
doaj   +1 more source

Animal Species Identification by PCR – RFLP of Cytochrome b

Scientific Papers Animal Science and Biotechnologies, 2023
An alternative DNA detection system is based on the polymerase chain reaction (PCR) amplification of a segment of the mitochondrial cytochrome b gene.
Tomáš Minarovič, Anna Trakovická, Alica Rafayová, Zuzana Lieskovská   +3 more
doaj  

Métodos subsidiários para o diagnóstico da Leishmaniose tegumentar americana (LTA): comparação dos resultados do seqüenciamento de DNA e da PCR-RFLP para determinação da espécie de leishmania em amostras cutâneo-mucosas Subsidiary methods for the diagnosis of American tegumentar leishmaniasis (ATL): comparison of sequencing of DNA and PCR-RFLP for identification of leishmania species in skin samples

Anais Brasileiros de Dermatologia, 2005
FUNDAMENTOS: Métodos moleculares têm-se mostrados mais eficazes para o diagnóstico da LTA. OBJETIVOS: Comparar os resultados da intradermorreação de Montenegro (IRM), presença de leishmania em biópsia (Bc), reação de imunofluorescência indireta (Rifi ...
Flávio C. Barbosa Garcia, Sandra Silva Rodrigues dos Santos, Maria Fernanda Chociay, Ângela C. Rapela Medeiros, Ana Maria F. Roselino   +4 more
doaj   +1 more source

Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages

Czech Journal of Food Sciences, 2017
The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in
Ivona Djurkin Kušec, Danijela Samac, Vladimir Margeta, Žarko Radišić, Dragutin Vincek, Goran Kušec   +5 more
doaj   +1 more source

IN SILICO DESIGN AND VALIDATION OF A NOVEL PCR-RFLP ASSAY FOR GPRC6A RS2247911 POLYMORPHISM INVOLVED IN MALE INFERTILITY [PDF]

Bulletin of Pharmaceutical Sciences. Assiut University, 2022
Male infertility is a prevalent disorder affecting approximately 7% of the male population, at least 2,000 genes are involved in spermatogenesis, availability of genetic investigations is a challenge in developing countries, GPRC6A, G-protein coupled ...
Kenda Jawich, Marwan Alhalabi, Sahar Al Fahoum   +2 more
doaj   +1 more source

Genes do eixo somatotrófico e características de crescimento numa população F2 de bovinos Somatotrophic axis genes on growth traits in a bovine F2 population

Pesquisa Agropecuária Brasileira, 2006
O objetivo deste trabalho foi avaliar a associação dos polimorfismos dos genes bGH, IGF-1 e PIT-1 com características de peso e ganho de peso numa população F2 de bovinos (Gir x Holandês), pela técnica de PCR-RFLP.
Marcos Vinicius Gualberto Barbosa da Silva, Mário Luiz Martinez, Marco Antonio Machado, Carlos Souza do Nascimento, Ana Lúcia Campos, Marta Fonseca Martins Guimarães, Ana Luisa Sousa Azevedo, Antônia Kécya França Moita, Jeffrey Frederico Lui   +8 more
doaj   +1 more source

Evolutionary Computation-Based Memetic Algorithm Against Genetic Algorithm to Improve PCR-RFLP Assay Primers of SNP Genotyping

IEEE Access, 2018
A genetic algorithm (GA) combines the restriction enzyme mining core of single nucleotide polymorphism (SNP) restriction fragment length polymorphism (RFLP) to design polymerase chain reaction (PCR)-RFLP primer pairs for SNP-based genotyping with ...
Yu-Huei Cheng, Ching-Ming Lai, Jiashen Teh, Che-Nan Kuo   +3 more
doaj   +1 more source

PCR‐RFLP typing of the bovine myoglobin gene

Animal Genetics, 1994
SummaryWe have identified substitutions in the 31 untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi‐allelic restriction fragment length polymorphism. Co‐dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction—restriction ...
Agaba, Morris, Kemp, Stephen J.
openaire   +3 more sources