Results 31 to 40 of about 655,509 (235)

Machine Learning Combined with Restriction Enzyme Mining Assists in the Design of Multi-Point Mutagenic Primers

Mathematics, 2022
The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and high specificity; thus, many small and medium laboratories use it to
Yu-Huei Cheng, Li-Yeh Chuang, Cheng-Hong Yang   +2 more
doaj   +1 more source

Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP and RFLP. [PDF]

PLoS ONE, 2016
BACKGROUND:The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid
Wen-Lin Liu, Huei-Chuan Shih, I-Szu Weng, Ya-Zhu Ko, Chi-Chu Tsai, Chang-Hung Chou, Yu-Chung Chiang   +6 more
doaj   +1 more source

Complex Genetic Architecture in RASopathies: Constitutional PTPN11 and Mosaic RIT1 Pathogenic Variants Underlying Severe Noonan Syndrome With Adult‐Onset Acute Myeloid Leukemia

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Noonan syndrome (NS) is a genetically heterogeneous disorder characterized by a broad spectrum of clinical features resulting from dysregulation of the RAS/MAPK pathway. Although complex genotypes are increasingly recognized in NS, cases harboring two distinct pathogenic variants in different NS genes remain extremely rare.
Francesco Prevedello, Dario Seif Ali, Chiara Piccolo, Chiara Rigon, Monica Forzan, Elena Tacchetto, Roberta Palmitessa, Davide Calosci, Leonardo Salviati, Carmela Gurrieri, Eva Trevisson   +10 more
wiley   +1 more source

PCR-RFLP typing of the p1 gene.

, 2019
M. pneumoniae isolates from 419 patients in this study were classified into eight p1 gene types by PCR-RFLP analysis. The RFLP patterns of the RepMP4 region (lanes 1 to 8) and the RepMP2/3 region (lanes 9 to 16) that represent eight p1 gene types are ...
Tsuyoshi Kenri (833481), Chihiro Katsukawa (6264050), Keigo Shibayama (183811), Kazuo Takahashi (182663)   +3 more
core   +1 more source

The Impact of Genetic Polymorphisms on Genotoxicity (DNA Damage) Among Children Exposed to Environmental Mutagens: A Systematic Review

Journal of Applied Toxicology, EarlyView.
ABSTRACT Children, as a particularly vulnerable group, are more susceptible to these mutagens due to their developing immune systems, higher physiological vulnerability, and increased exposure through behaviors like outdoor play and hand‐to‐mouth activities. This review aims to investigate the relationship between these environmental exposures, genetic
Thiago Guedes Pinto, Daniel Vitor de Souza, Gabriel Carvalhal de Aguiar, Ana Claudia Muniz Renno, Patricia Ramos Cury, Jean Nunes dos Santos, Rogerio Aparecido Dedivitis, Daniel Araki Ribeiro   +7 more
wiley   +1 more source

Performance Evaluation of a Premier Resolution HPLC System for Detecting Hemoglobin Constant Spring, Hemoglobin Paksé, and Coexisting α‐ and β‐Thalassemia Mutations

Journal of Clinical Laboratory Analysis, EarlyView.
This study compared Premier Resolution HPLC with Capillary Electrophoresis for detecting HbCS/Hb Paksé variants and coexisting thalassemia mutations. Premier Resolution HPLC demonstrated superior performance with 50% fewer false negative cases (11 vs. 22) compared to Capillary Electrophoresis, particularly in heterozygous cases.
Surada Satthakarn, Wibhasiri Srisuwan, Sitthichai Panyasai   +2 more
wiley   +1 more source

PCR-RFLP detection results of the three new SNPs (g.-1293C>G, g.56T>C, g.7017C>T) in bovine C3 gene.

, 2022
PCR-RFLP detection results of the three new SNPs (g.-1293C>G, g.56T>C, g.7017C>T) in bovine C3 gene.
Yonghui Wang (287491), Jingpeng Wang (1877023), Jingming Huang (12982031), Changfa Wang (390394), Yan Sun (22436), Chunhong Yang (764312), Yuhua Li (52681), Junyu Zhai (819072), Zhihua Ju (390391)   +8 more
core   +1 more source

Leaf micromorphology and genetic diversity analysis of Iranian Hordeum species using ISSR and SCoT markers

Nordic Journal of Botany, EarlyView.
The genus Hordeum (Poaceae), of the tribe Triticeae, comprises approximately 32 species with substantial potential for barley improvement. Hordeum vulgare is an economically important cereal widely cultivated across diverse environments, from the Arctic to desert and humid regions.
Maryam Keshavarzi, Raheleh Tabaripour, Nasrin Farasat   +2 more
wiley   +1 more source

Molecular identification of Candida species isolated from cases of neonatal candidemia using polymerase chain reaction-restriction fragment length polymorphism in a tertiary care hospital

Indian Journal of Pathology and Microbiology, 2017
Context: Candida spp. is an emerging cause of bloodstream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs in various Candida species are responsible for the increase in morbidity ...
Akeela Fatima, Gulnaz Bashir, Tehmeena Wani, Abiroo Jan, Amrish Kohli, Mosin S Khan   +5 more
doaj   +1 more source

Sensitivity of Irish Pyrenopeziza brassicae populations to methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI) and succinate dehydrogenase inhibitor (SDHI) fungicides

Pest Management Science, EarlyView.
For three collections of Irish Pyrenopeziza brassicae populations fungicide sensitivity status to methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI) and succinate dehydrogenase inhibitor (SDHI) fungicides was determined using sensitivity screening in vitro. Molecular mechanisms of insensitivity have been identified for MBC and QoI in
Diana E Bucur, Yong‐Ju Huang, Bruce DL Fitt, Steven Kildea   +3 more
wiley   +1 more source