Results 31 to 40 of about 43,727 (186)

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases, 2011
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD   +4 more
doaj   +1 more source

Sensitivity of Irish Pyrenopeziza brassicae populations to methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI) and succinate dehydrogenase inhibitor (SDHI) fungicides

Pest Management Science, EarlyView.
For three collections of Irish Pyrenopeziza brassicae populations fungicide sensitivity status to methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI) and succinate dehydrogenase inhibitor (SDHI) fungicides was determined using sensitivity screening in vitro. Molecular mechanisms of insensitivity have been identified for MBC and QoI in
Diana E Bucur, Yong‐Ju Huang, Bruce DL Fitt, Steven Kildea   +3 more
wiley   +1 more source

Target‐site mutations and phenotypic failure in Myzus persicae against pyrethroids and carbamates in central Europe

Pest Management Science, EarlyView.
Molecular screening of 140 field‐collected Myzus persicae aphids revealed target‐site mutations L1014F, M918L, M918T, and L932F in the sodium channel and S431F in Ace2. The M918L mutation was the primary driver of high pyrethroid (cypermethrin) resistance, while S431F served as a definitive marker for carbamate (pirimicarb) resistance.
Jitka Stara, Jan Hubert, Pavla Pabiskova, Frantisek Kocourek   +3 more
wiley   +1 more source

Hybridization With a Non‐Native Congener Threatens the Catalan Chub (Squalius laietanus): Optimization of a DNA‐Based Early Warning Tool

Integrative Zoology, EarlyView.
Hybridization with non‐native congeners threatens endemic freshwater fishes. Using a rapid and cost‐effective nuclear DNA assay, we detected widespread hybridization between the Catalan chub (Squalius laietanus) and the non‐native European chub (Squalius cephalus) across S.
Núria Pérez‐Bielsa, Alba Abras, Sandra Heras, Marc Palmada‐Flores, Jose‐Luis García‐Marín   +4 more
wiley   +1 more source

Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

International Journal of Microbiology, 2014
Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals.
Nancy Younis Omar, Hala Abdel Salam Ali, Reem Abdel Hameed Harfoush, Engy Hamdy El Khayat   +3 more
doaj   +1 more source

Characterization of 12S rRNA gene for meat identification of common wild and domestic small herbivores as an aid to wildlife forensic

Veterinary World, 2013
Aim: Chital and sambar are the common wild small herbivores, which are vulnerable to poaching for their meat. Many times poachers claim the wild meat to be that of goat or sheep. Hence, authentic evidences are required to stop such wildlife crime.
E. Joseph, Sanjeev Singh, Rakesh Ranjan, S.N.S. Parmar, Nidhi Rajput, A.B. Shrivastav   +5 more
doaj   +1 more source

Discrimination of Kratom Products by an Improved PCR-RFLP Method

YAKUGAKU ZASSHI, 2020
In Japan, mitragynine, 7-hydroxymitragynine and Mitragyna speciosa Korth. (M. speciosa, "Kratom") were controlled as Designated Substances under the Pharmaceutical and Medical Device Act from March 2016. In this study, the origins of 16 Kratom products obtained from the illegal drug market in Japan were investigated by DNA analyses and LC-MS analyses ...
Jun, Ogata, Maiko, Kawamura, Takashi, Hakamatsuka, Ruri, Kikura-Hanajiri   +3 more
openaire   +3 more sources

Impact of FSHB and FSHR Genes Polymorphisms on Hormonal Profile and Sperm Retrieval Outcome in Men With Klinefelter Syndrome: A Clinical–Genetic Predictive Study

Andrology, EarlyView.
ABSTRACT Background Genetic variability within the follicle‐stimulating hormone (FSH)‐related genes might contribute to phenotypic heterogeneity in patients with Klinefelter syndrome (KS), yet its clinical impact on sperm retrieval remains unclear. Objectives To investigate the association between FSHB c.211 G > T and FSHR polymorphisms (c.2039 A > G ...
Andrea Graziani, Giuseppe Grande, Maria Santa Rocca, Massimo Iafrate, Riccardo Selice, Antonella Di Mambro, Alberto Ferlin   +6 more
wiley   +1 more source

DETERMINACIÓN DE LA MUTACIÓN S315T DEL GEN KATG EN AISLADOS RESISTENTES A ISONIACIDA DE MYCOBACTERIUM TUBERCULOSIS MEDIANTE PCR-RFLP

Infectio, 2018
Objetive: To determine the S315T mutation of the katG gene in Mycobacterium tuberculosis isoniazid-resistant isolates by PCR-RFLP. Materials and Methods: Polymorphism analysis of 1054 and 630 bp products containing the S315T mutation of the katG gene ...
Jenny Sánchez-Domínguez
doaj   +1 more source

Genotyping Of ABO gene In Iraqi Population Sample By Using PCR-RFLP Technique [PDF]

مجلة جامعة الانبار للعلوم الصرفة, 2017
The aim of this study was the molecular detection of ABO gene and identify the patterns of alleles in Iraqi population sample. The study consisted of 100 unrelated individuals who appear to be healthy.
Qabas H. Alawee, Louay M. Abdou, Saad M. AL-Nida   +2 more
doaj   +1 more source