Results 41 to 50 of about 655,509 (235)

PCR primers for PCR-RFLP assays.

, 2014
PCR primers for PCR-RFLP assays.
Wei Li (7081), Zhongde Wang (476556), Bi Shi (641952), Zhiqiang Fan (641950), Kenneth L. White (641953), Thomas D. Bunch (453210), Sang R. Lee (641951), Il-Keun Kong (85884), Qinggang Meng (511759)   +8 more
core   +1 more source

Simultaneous detection of TB and drug resistance to Isoniazid in Mycobacterium tuberculosis clinical isolates using PCR-RFLP method

Iranian South Medical Journal, 2015
Background: Early detection and suitable drug admission are the first attempts to control of TB and MDR-TB. In this study, PCR-RFLP method was used for rapid detection of Mycobacterium tuberculosis and resistance to isoniazid.
Maryam alsadat Tayeboon, Maryam Sadrnia, Hamidreza Mohajerani   +2 more
doaj  

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases, 2011
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD   +4 more
doaj   +1 more source

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS‐PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR/Cas9 editing

Pest Management Science, EarlyView.
A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos, Athanasios Petmezas, Anastasios Samaras, George Karaoglanidis   +3 more
wiley   +1 more source

Enterococcus faecalis and Endodontic Treatment Failure: A Qualitative Systematic Review of Its Role and Elimination Strategies

Australian Endodontic Journal, EarlyView.
ABSTRACT The aim of this study was to synthesize evidence on the influence of Enterococcus faecalis on endodontic treatment failure and strategies for its elimination. This study followed the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines and was registered in PROSPERO (CRD420251022741).
Francine Santos Fernandes de Lima, Analia Gabriella Borges Ferraz Facury   +1 more
wiley   +1 more source

Discrimination of Kratom Products by an Improved PCR-RFLP Method

YAKUGAKU ZASSHI, 2020
In Japan, mitragynine, 7-hydroxymitragynine and Mitragyna speciosa Korth. (M. speciosa, "Kratom") were controlled as Designated Substances under the Pharmaceutical and Medical Device Act from March 2016. In this study, the origins of 16 Kratom products obtained from the illegal drug market in Japan were investigated by DNA analyses and LC-MS analyses ...
Jun, Ogata, Maiko, Kawamura, Takashi, Hakamatsuka, Ruri, Kikura-Hanajiri   +3 more
openaire   +3 more sources

Impact of FSHB and FSHR Genes Polymorphisms on Hormonal Profile and Sperm Retrieval Outcome in Men With Klinefelter Syndrome: A Clinical–Genetic Predictive Study

Andrology, EarlyView.
ABSTRACT Background Genetic variability within the follicle‐stimulating hormone (FSH)‐related genes might contribute to phenotypic heterogeneity in patients with Klinefelter syndrome (KS), yet its clinical impact on sperm retrieval remains unclear. Objectives To investigate the association between FSHB c.211 G > T and FSHR polymorphisms (c.2039 A > G ...
Andrea Graziani, Giuseppe Grande, Maria Santa Rocca, Massimo Iafrate, Riccardo Selice, Antonella Di Mambro, Alberto Ferlin   +6 more
wiley   +1 more source

Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

International Journal of Microbiology, 2014
Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals.
Nancy Younis Omar, Hala Abdel Salam Ali, Reem Abdel Hameed Harfoush, Engy Hamdy El Khayat   +3 more
doaj   +1 more source

DETERMINACIÓN DE LA MUTACIÓN S315T DEL GEN KATG EN AISLADOS RESISTENTES A ISONIACIDA DE MYCOBACTERIUM TUBERCULOSIS MEDIANTE PCR-RFLP

Infectio, 2018
Objetive: To determine the S315T mutation of the katG gene in Mycobacterium tuberculosis isoniazid-resistant isolates by PCR-RFLP. Materials and Methods: Polymorphism analysis of 1054 and 630 bp products containing the S315T mutation of the katG gene ...
Jenny Sánchez-Domínguez
doaj   +1 more source

A novel selective stabilizer of the ryanodine receptor 2 prevents stress‐induced ventricular arrhythmias without impairing cardiac function

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Aberrant activation of type 2 ryanodine receptors (RyR2) causes lethal arrhythmias, such as catecholaminergic polymorphic ventricular tachycardia (CPVT). Developing drugs that suppress RyR2 hyperactivation may be key to novel arrhythmia treatments.
Nagomi Kurebayashi, Masami Kodama, Hana Inoue, Masato Konishi, Masami Sugihara, Takashi Murayama, Ryosuke Ishida, Koichiro Ishii, Shuichi Mori, Yukari Endo, Xi Zeng, Yukiko U. Inoue, Takayoshi Inoue, Satoru Noguchi, Hajime Nishio, Eri Nakashima, Norio Suzuki, Noriko Nakaya, Ryushi Sato, Yuya Otori, Ayuna Tsutsumi, Mirei Takahashi, Takayuki Oka, Haruo Ogawa, Kayoko Kanamitsu, Hiroyuki Kusuhara, Utako Yokoyama, Junko Kurokawa, Hiroyuki Kagechika, Takashi Sakurai   +29 more
wiley   +1 more source