Results 41 to 50 of about 1,381,507 (269)

Nobody Is Perfect: Comparison of the Accuracy of PCR-RFLP and KASP™ Method for Genotyping. ADH1B and FTO Polymorphisms as Examples.

Folia biologica, 2015
DNA genotyping is among the most common analyses currently performed in scientific research. Two high-throughput genotyping techniques are widely used - the "classic" PCR-RFLP and probe-based methods such as TaqMan® PCR assay or KASP™ genotyping.
J. Hubacek, H. Pikhart, A. Peasey, R. Kubínová, M. Bobák   +4 more
semanticscholar   +1 more source

Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP and RFLP. [PDF]

PLoS ONE, 2016
BACKGROUND:The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid
Wen-Lin Liu, Huei-Chuan Shih, I-Szu Weng, Ya-Zhu Ko, Chi-Chu Tsai, Chang-Hung Chou, Yu-Chung Chiang   +6 more
doaj   +1 more source

Identification of the Kna/Knb polymorphism and a method for Knops genotyping [PDF]

, 2004
BACKGROUND: DNA mutations resulting in the McCoy and Swain-Langley polymorphisms have been identified on complement receptor 1 (CR1)—a ligand for rosetting of Plasmodium falciparum-infected RBCs.
Daniels, Helgeson, Krych-Goldberg, Mallan, Molthan, Molthan, Moulds, Moulds, Moulds, Moulds, Moulds, Moulds, Moulds, Moulds, Rao, Rowe, Tamasauskas, Zimmerman   +17 more
core   +1 more source

Machine Learning‐Augmented Loop‐Mediated Isothermal Amplification‐Enabled Point‐of‐Care for Mpox‐Specific Detection

Advanced Intelligent Systems, EarlyView.
A low‐cost, portable point‐of‐care platform for rapid Mpox detection using loop‐mediated isothermal amplification is reported. The device integrates fluorescence readout and mobile monitoring. A machine‐learning model analyzes temperature data and correlates thermal changes with DNA concentration, enabling sensitive and reliable molecular diagnosis in ...
Nazente Atceken, Ahmed Choukri Abdullah, Olgac Ozarslan, Ziya Yıldız, Savas Tasoglu   +4 more
wiley   +1 more source

MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]

, 2002
Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
., Sutarno, A.J, Lymbery, J.M, Cummins, RCA, Thompson   +3 more
core  

Complex Genetic Architecture in RASopathies: Constitutional PTPN11 and Mosaic RIT1 Pathogenic Variants Underlying Severe Noonan Syndrome With Adult‐Onset Acute Myeloid Leukemia

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Noonan syndrome (NS) is a genetically heterogeneous disorder characterized by a broad spectrum of clinical features resulting from dysregulation of the RAS/MAPK pathway. Although complex genotypes are increasingly recognized in NS, cases harboring two distinct pathogenic variants in different NS genes remain extremely rare.
Francesco Prevedello, Dario Seif Ali, Chiara Piccolo, Chiara Rigon, Monica Forzan, Elena Tacchetto, Roberta Palmitessa, Davide Calosci, Leonardo Salviati, Carmela Gurrieri, Eva Trevisson   +10 more
wiley   +1 more source

Genetic diversity of african and worldwide strains of Ralstonia solanacearum as determined by PCR-Restriction Fragment Length Polymorphism analysis of the hrp gene region [PDF]

, 1999
The genetic diversity among a worldwide collection of 120 strains of #Ralstonia solanacearum# was assessed by restriction fragment length polymorphism (RFLP) analysis of amplified fragments from the hrp gene region.
Poussier, Stéphane, Vandewalle, P., Luisetti, Jacques   +2 more
core  

The Impact of Genetic Polymorphisms on Genotoxicity (DNA Damage) Among Children Exposed to Environmental Mutagens: A Systematic Review

Journal of Applied Toxicology, EarlyView.
ABSTRACT Children, as a particularly vulnerable group, are more susceptible to these mutagens due to their developing immune systems, higher physiological vulnerability, and increased exposure through behaviors like outdoor play and hand‐to‐mouth activities. This review aims to investigate the relationship between these environmental exposures, genetic
Thiago Guedes Pinto, Daniel Vitor de Souza, Gabriel Carvalhal de Aguiar, Ana Claudia Muniz Renno, Patricia Ramos Cury, Jean Nunes dos Santos, Rogerio Aparecido Dedivitis, Daniel Araki Ribeiro   +7 more
wiley   +1 more source

Species Differentiation Of Fish Samples By Restriction Fragment Length Polymorphism Analysis Of Cytochrome B Gene [PDF]

, 2009
Metode pengukuran polimorfisme fragmen hasil pemotongan produkreaksi polimorfik berantai oleh enzim restriksi spesifik (polymerase chainreaction-restriction fragment length polymorphism, RFLP-PCR) telah digunakanuntuk membedakan beberapa jenis ikan ...
I, Z. M. (Zuraini), R, S. (Son), R, T. (Tunung), Y, H. (Haryani)   +3 more
core  

PCR‐RFLP typing of the bovine myoglobin gene

Animal Genetics, 1994
SummaryWe have identified substitutions in the 31 untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi‐allelic restriction fragment length polymorphism. Co‐dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction—restriction ...
Agaba, Morris, Kemp, Stephen J.
openaire   +3 more sources