Results 21 to 30 of about 1,381,507 (269)

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

Journal of Applied Biotechnology Reports, 2019
Polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) and PCR–restriction fragment length polymorphism (PCR-RFLP) are two independent methods used in the post-amplification genotyping of DNA variations.
H. Hashim, M. Al-Shuhaib
semanticscholar   +1 more source

A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

Journal of Analytical Methods in Chemistry, 2018
There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species.
F. Guan, Yu-Ting Jin, Jin Zhao, Aichun Xu, Yuan-Yuan Luo   +4 more
semanticscholar   +1 more source

Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP

Tropical Medicine and Health, 2017
BackgroundThe development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French ...
S. Simon, Mathieu Nacher, B. Carme, C. Basurko, A. Roger, A. Adenis, M. Ginouves, M. Demar, P. Couppié   +8 more
semanticscholar   +1 more source

Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations.

Diagnostic microbiology and infectious disease, 2018
Hsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leishmania species. In this study, we used a previously described hsp70 PCR-RFLP method for
Caroline R. Espada, P. A. Ortiz, J. Shaw, A. Barral, J. Costa, Silvia R. B. Uliana, A. Coelho   +6 more
semanticscholar   +1 more source

Functional plasticity in the type IV secretion system of Helicobacter pylori. [PDF]

, 2012
Helicobacter pylori causes clinical disease primarily in those individuals infected with a strain that carries the cytotoxin associated gene pathogenicity island (cagPAI).
Barrozo, Roberto M, Cariaga, Taryn A, Cooke, Cara L, Cover, Timothy L, Gaddy, Jennifer A, Hansen, Lori M, Johnson, Elizabeth M, Lam, Anna M, Peek, Richard M, Solnick, Jay V, Suarez, Giovanni   +10 more
core   +1 more source

Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping [PDF]

, 2015
The Kidd blood group antigens, Jkª and Jkᵇ , are two of the main surface markers which are found on the membrane of red blood cells. The determination of whether a donor or a recipient has the Jkª and/or the Jkᵇ antigens is crucially important to ...
Borg, Joseph J., Debono, Jesmond, Xuereb, Karl   +2 more
core   +1 more source

Identification of Eutypa lata by PCR-RFLP [PDF]

Plant Disease, 2004
Eutypa lata is a vascular canker pathogen of woody plants commonly diagnosed by isolating the pathogen from infected tissue. Related fungi from the same family, the Diatrypaceae, also have been found in association with grapevine in Californian vineyards. An in situ polymerase chain reaction (PCR) method has been developed for detection of E.
P E, Rolshausen, F, Trouillas, W D, Gubler   +2 more
openaire   +2 more sources

Species relationships in the genus Vasconcellea (Caricaceae) based on molecular and morphological evidence [PDF]

, 2005
Validity of the taxa currently recognized in the genus Vasconcellea was analyzed by investigating morphological and molecular data from 105 specimens of this genus and six specimens of the related genus Carica. Taxon identification of these specimens was
Gheysen, Godelieve, Goetghebeur, Paul, Kyndt, Tina, Romeijn-Peeters, Eliza, Romero-Motochi, José P, Van Droogenbroeck, Bart   +5 more
core   +2 more sources

Molecular surveillance of Theileria parasites of livestock in Oman [PDF]

, 2017
Background: Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian Peninsula, and causes high rates of mortality and morbidity in sheep and cattle.
Al-Fahdi, Amira, Al-Hamidhi, Salama, Alqamashoui, Badar, Babiker, Hamza, Bilgic, Huseyin Bilgin, Bobade, Patrick, Hussain, Abdel-Rahim, Johnson, Eugene H., Karagenc, Tulin, Kose, Onur, Shiels, Brian, Tageldin, Mohammed H., Tait, Andy   +12 more
core   +1 more source

Molecular Characterization of Cryptosporidium spp. in Wild Rodents of Southwestern Iran Using 18s rRNA Gene Nested-PCR-RFLP and Sequencing Techniques

Journal of Tropical Medicine, 2016
Background. Rodents could act as reservoir for Cryptosporidium spp. specially C. parvum, a zoonotic agent responsible for human infections. Since there is no information about Cryptosporidium infection in rodents of Ahvaz city, southwest of Iran, hence ...
J. Saki, M. Foroutan-Rad, Reza Asadpouri
semanticscholar   +1 more source