Results 21 to 30 of about 43,727 (186)

Molecular Genotyping of HBV by using Nested PCR-RFLP among Hepatitis B Patients in Daerah Istimewa Yogyakarta Province and Surrounding Area

Indonesian Journal of Biotechnology, 2008
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto, Nenny Sri Mulyani, Titis Widowati, Nastiti Wijayanti, Purnomo Hadi   +4 more
doaj   +1 more source

Machine Learning Combined with Restriction Enzyme Mining Assists in the Design of Multi-Point Mutagenic Primers

Mathematics, 2022
The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and high specificity; thus, many small and medium laboratories use it to
Yu-Huei Cheng, Li-Yeh Chuang, Cheng-Hong Yang   +2 more
doaj   +1 more source

Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP and RFLP. [PDF]

PLoS ONE, 2016
BACKGROUND:The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid
Wen-Lin Liu, Huei-Chuan Shih, I-Szu Weng, Ya-Zhu Ko, Chi-Chu Tsai, Chang-Hung Chou, Yu-Chung Chiang   +6 more
doaj   +1 more source

Molecular methods for differentiating Phragmites australis subspecies and identification of hybrid stands in the Upper Midwestern United States

Applications in Plant Sciences, EarlyView.
Abstract Premise In North America, Phragmites australis (common reed) has drawn a great deal of research attention. Non‐native P. australis subsp. australis is a noxious weed that has locally displaced native P. australis subsp. americanus in some areas.
Nicholas P. Tippery, Daniel J. Larkin, Julia Bohnen, Douglas L. Wendell, Neil O. Anderson, Susan M. Galatowitsch, Marie Abbey, Alan G. Smith, Andrzej K. Noyszewski   +8 more
wiley   +1 more source

The Impact of Genetic Polymorphisms on Genotoxicity (DNA Damage) Among Children Exposed to Environmental Mutagens: A Systematic Review

Journal of Applied Toxicology, EarlyView.
ABSTRACT Children, as a particularly vulnerable group, are more susceptible to these mutagens due to their developing immune systems, higher physiological vulnerability, and increased exposure through behaviors like outdoor play and hand‐to‐mouth activities. This review aims to investigate the relationship between these environmental exposures, genetic
Thiago Guedes Pinto, Daniel Vitor de Souza, Gabriel Carvalhal de Aguiar, Ana Claudia Muniz Renno, Patricia Ramos Cury, Jean Nunes dos Santos, Rogerio Aparecido Dedivitis, Daniel Araki Ribeiro   +7 more
wiley   +1 more source

Development and Analytical Validation of a Laboratory‐Customized TaqMan‐MGB Probe Method for CYP2C19 Genotyping

Journal of Clinical Laboratory Analysis, EarlyView.
A laboratory‐customized, open‐sequence TaqMan‐MGB probe assay was developed for detecting CYP2C19*2 and CYP2C19*17 polymorphisms using dried blood spot‐derived DNA. The assay demonstrated allele‐specific discrimination, candidate LODs of 1.17 × 102 copies/μL for CYP2C19*2 and 0.94 × 103 copies/μL for CYP2C19*17, and repeatability with CVs below 10%. In
Yaqun Liu, Lianghui Chen, Xuanyi Zheng, Peikui Yang, Yicun Chen, Chengsong Xie, Zhenxia Zhang, Yuzhong Zheng   +7 more
wiley   +1 more source

DNA Extracted From Aged Formalin‐Fixed Paraffin‐Embedded Specimens of Patients With Papillary Thyroid Carcinomas Can Be Used for Whole Exome Sequencing

Journal of Clinical Laboratory Analysis, EarlyView.
We aimed to assess the quality of DNA extracted from long‐term stored FFPE specimens of patients with papillary thyroid carcinoma from three hospitals in Hiroshima and their applicability to whole exome sequencing. FFPE samples preserved for up to 55 years may be amenable to sequencing with increased read depth.
Kousuke Tanimoto, Tomonori Hayashi, Hidewaki Nakagawa, Kazuhiro Maejima, Koji Arihiro, Megumu Fujihara, Takashi Nishisaka, Masahiro Ito, Masahiro Nakashima, Naohiro Kato, Zhenqiu Liu, Misa Imaizumi, Alina Brenner, Stephen J. Chanock, Osamu Tanabe, Kotaro Ozasa   +15 more
wiley   +1 more source

Molecular identification of Candida species isolated from cases of neonatal candidemia using polymerase chain reaction-restriction fragment length polymorphism in a tertiary care hospital

Indian Journal of Pathology and Microbiology, 2017
Context: Candida spp. is an emerging cause of bloodstream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs in various Candida species are responsible for the increase in morbidity ...
Akeela Fatima, Gulnaz Bashir, Tehmeena Wani, Abiroo Jan, Amrish Kohli, Mosin S Khan   +5 more
doaj   +1 more source

Leaf micromorphology and genetic diversity analysis of Iranian Hordeum species using ISSR and SCoT markers

Nordic Journal of Botany, EarlyView.
The genus Hordeum (Poaceae), of the tribe Triticeae, comprises approximately 32 species with substantial potential for barley improvement. Hordeum vulgare is an economically important cereal widely cultivated across diverse environments, from the Arctic to desert and humid regions.
Maryam Keshavarzi, Raheleh Tabaripour, Nasrin Farasat   +2 more
wiley   +1 more source

Simultaneous detection of TB and drug resistance to Isoniazid in Mycobacterium tuberculosis clinical isolates using PCR-RFLP method

Iranian South Medical Journal, 2015
Background: Early detection and suitable drug admission are the first attempts to control of TB and MDR-TB. In this study, PCR-RFLP method was used for rapid detection of Mycobacterium tuberculosis and resistance to isoniazid.
Maryam alsadat Tayeboon, Maryam Sadrnia, Hamidreza Mohajerani   +2 more
doaj