Results 31 to 40 of about 161,067 (287)
Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette [PDF]
Iranian Journal of Basic Medical Sciences, 2017 Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function.
Mehdi Imani +3 more
doaj +1 more source
Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters [PDF]
, 2010 Random mutagenesis has been used to identify the target DNA sites for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters. The malX promoter is repressed by MalI binding to a DNA site located from position -24 to position -9 ...
Barnard +22 more
core +1 more source
Journal of Lipid Research, 1996 Several lipoprotein lipase (LPL) gene polymorphisms have been found associated with fasting lipid levels, but their impact on coronary heart disease (CHD) is less clearly established.
R Jemaa +9 more
doaj +1 more source
Different fates of the chloroplast tufA gene following its transfer to the nucleus in green algae [PDF]
, 1990 Previous work suggested that the tufA gene, encoding protein synthesis elongation factor Tu, was transferred from the chloroplast to the nucleus within the green algal lineage giving rise to land plants.
Baldauf, S.L. +2 more
core +4 more sources
Development of a new bicistronic retroviral vector with strong IRES activity
BMC Biotechnology, 2006 Background Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the monitoring of expression of a ...
Boulukos Kim E +4 more
doaj +1 more source
Transformation of the Methylotrophic Actinomycete Amycolatopis methanolica with Plasmid DNA: Stimulatory Effect of a pMEA300-Encoded Gene [PDF]
, 1995 Amycolatopsis methanolica contains a 13.29-kb plasmid (pMEA300) present both in the free state and integrated at a unique genomic location. A pMEA300-free derivative (strain WV1) was selected, allowing further analysis of pMEA300-encoded functions. Whole
Dijkhuizen, L., +2 more
core +2 more sources
Ciência Rural, 1996 The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to ...
Sergio Echeverrigaray +2 more
doaj +1 more source
Amplification and Cloning of alfa-Toxin Gene from Clostridium Perfringenes Bacterium in E. coli [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective The aim of this study was to amplify and cloning complete, as well as the primary part of the α-toxin gene from Clostridium perfringens with the size of 1100 and 750 bps, in E. coli using the expression vector pET28a.
Hosseinali Sasan +5 more
doaj +1 more source
Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI
Nucleic Acids Research, 1978 The cleavage of the DNAs of the B95-8 and P3HR-1 virus strains of Epstein-Barr virus by the restriction endonucleases EcoRI, HindIII and BamI was investigated using a new technique for quantitative evaluation of the fluorescence of ethidium stained DNA fragments separated on agarose gels.
Lars Rymo, Sven Forsblom
openalex +5 more sources
HindIII polymorphism in the humanc-sisproto-oncogene
Nucleic Acids Research, 1988 The 2.0 kb BamH1 restriction fragment corresponding to a cDNA insert encoding the human c-sis PDGF A chain and nucleotide sequences homologous to the v-sis gene was isolated from plasmid pSM-1. An identical polymorphism was noted using the 1.2 kb PstI fragment or the 1.0 kb PstI/XbaI fragment isolated from the v-sis sequence subcloned in the plasmid pV-
R.M. Fourney +3 more
openalex +5 more sources