Results 1 to 10 of about 5,087 (131)

Combined biochemistry and histocytochemistry as a tool to investigate Ecto‐ATPase in the cardiac muscle [PDF]

Microscopy Research and Technique, 2002
AbstractEcto‐ATPase (ecto‐adenosine triphosphatase), a key enzyme of cardiac metabolism, is responsible for modulation of the concentration of extracellular nucleotides in the heart. We present methodology consisting of the combined use of biochemical and histocytochemical techniques to study its properties.
Vadim S. Zinchuk, Teruhiko Okada, Harumichi Seguchi   +2 more
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Experimental study for the toxicological effects of SVATE-3 on rat kidney. Observation by light and electron microscope and by enzyme histocytochemistry.

ACTA HISTOCHEMICA ET CYTOCHEMICA, 1992
In the present study, we have done an acute toxicological experiment on the rat liver by administration of SVATE-3 in clinical doses (high and low dose, respectively) for 3 weeks. By using morphological methods, light and electron microscopy, enzyme histocytochemistry in light and electron microscope and biochemistry, we have observed the histological ...
Yu Shi, Li Liu, Wei He, SHU WEN ZHU, Qi Wan, Jun Gao, DONG JAN LIU, Zhan‐You Wang, Yan Xu, YU KUEN GUO, WEN XEU HAO   +10 more
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Studies on monoamine-containing neurons by immunoenzyme-histocytochemistry and immunohistocytochemistry with special reference to gold-fish brain.

ACTA HISTOCHEMICA ET CYTOCHEMICA, 1984
In the goldfish brain, eleven groups of tyrosine hydroxylase (TH) -positive (catecholaminergic neurons and four groups of serotonin (5-HT) -positive (serotonergic) neurons are described as follows by immunofluorescence and light microscopic level of PAP technique: 1) The olfactory bulb (TH); 2) The area ventralis telencephali (TH); 3) The nucleus ...
Ikuko Nagatsu, Nobuyuki Karasawa, Yukari Kawakami, Masami Yoshida   +3 more
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Microwave-stimulated fixation for histocytochemistry: Application to surgical pathology and preembedding immunoelectron microscopy.

ACTA HISTOCHEMICA ET CYTOCHEMICA, 1990
Microwave (MW)-stimulated fixation was applied to surgical pathology and preembedding immunoelectron microscopy. Fresh specimens were microwavedin the fixatives for 15-30sec using a conventional MW oven (500W) containing 200ml of water load. The temperature of fixatives was raised to 35-45°C.
Haruo Ohtani, Hiroshi Naganuma, Hiroshi Nagura   +2 more
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2. Histocytochemistry Series The Fundamentals of Immunoelectron Microscopy (3)

Nihon Ika Daigaku Igakkai Zasshi, 2009
Immunohistochemistry is concerned with the detection of specific biological substances at the light and electron microscopic levels with antibodies labeled with visible markers, such as horseradish peroxidase and colloidal gold. In particular, the immunohistochemistry of electron microscopy has provided much morphological and biological information ...
Hitoshi Ozawa, Toshiyuki Matsuzaki
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Experimental study for the toxicological effects of SVATE-3 on rat liver. Observation by light and electron microscope and by enzyme histocytochemistry.

ACTA HISTOCHEMICA ET CYTOCHEMICA, 1990
In the present study, we have done an acute toxicological experiment on the rat liver by administration of SVATE-3 in clinical doses (high and low dose, respectively) for 3 weeks. By using morphological methods, light and electron microscopy, enzyme histocytochemistry in light and electron microscope and biochemistry, we have observed the histological ...
Yuxiu Shi, Li Liu, Wei He, Yan Wang, Dongjian Liu, Qi Wan, Zhan‐You Wang, Wei Liu, Yanping Xu, WENXEU HAO   +9 more
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2. Histocytochemistry Series The Basic Knowledge for the Analysis of Clinicopathological Features (7)

Nihon Ika Daigaku Igakkai Zasshi, 2011
The basic knowledge of treating clinicopathological features is essential for analysis. In this note, we describe procedures for the analysis of clinicopathological features, with a focus on statistics.
Ichiro Akagi, Masao Miyashita, Eiji Uchida, Toshihiro Takizawa   +3 more
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2. Histocytochemistry Series In Situ Hybridization Using Non-radioisotope labeled RNA Probes (4)

Nihon Ika Daigaku Igakkai Zasshi, 2010
In situ hybridization is a powerful method directed toward obtaining spatial and temporal information concerning the expression and distribution of RNA molecules (e.g., messenger RNA and microRNA) in vivo. In this technical note, we describe procedures for in situ hybridization with non-radioisotope labeled RNA probes, especially a digoxigenin-labeled ...
Takuya Mishima, Shanshun Luo, Toshiyuki Takeshita, Toshihiro Takizawa   +3 more
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2. Histocytochemistry Series Morphological and Functional Analyses of Cells, Using Confocal Laser Scanning Microscopy System: Example for Morphological and Functional Analyses of Cancer Cells, Using Multi-mode Microscopy System (2)

Nihon Ika Daigaku Igakkai Zasshi, 2009
The confocal laser scanning microscope (CLSM) is a device for obtaining high-resolution optical images of immunofluorescent staining. The CLSM can produce in-focus images of thick specimens, a process known as optical sectioning. The images are reconstructed with a computer, using 3-dimensional image software, allowing 3-dimensional reconstructions of ...
Zenya Naito
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2. Histocytochemistry Series Immunohistochemistry in Pathology (5)

Nihon Ika Daigaku Igakkai Zasshi, 2010
Immunohistochemistry is a technique to examine the expression and distribution of biomolecules in situ using antibodies labeled with enzymes, such as horseradish peroxidase. Generally, both immunohistochemistry and immunofluorescence are termed as immunostaining, because localized biomolecules in situ are detected with antigen-antibody immunoreactions ...
Akira Shimizu, Kyoko Wakamatsu, Yukinari Masuda, Yuh Fukuda   +3 more
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