Advanced Science, EarlyView.A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son +2 more
wiley +1 more source
Randomized trial of multi-strain <i>Lactobacillus crispatus</i> vaginal live biotherapeutic products after antibiotic therapy for bacterial vaginosis: study protocol for VIBRANT (vaginal lIve biotherapeutic RANdomized trial). [PDF]
Contemp Clin Trials CommunChetty C +19 more
europepmc +1 more source
Advanced Science, EarlyView.G9a, and DNA Methyltransferase1 (DNMT1) cooperatively modulates E2F1 on the promoter of tumor suppressor p53‐binding protein 2 (TP53BP2) increased autophagy in preeclampsia. TP53BP2 promotes autophagy in trophoblasts through DNA methylation and H3K9me2‐mediated transcriptional regulation.
Nan Jiang +12 more
wiley +1 more source
Mathematical Prediction for Geometry‐Mediated Cell 3D In‐Growth on Bone Tissue Engineering Scaffolds
Advanced Science, EarlyView.This study identifies a fundamental pore size dependent pattern of three dimensional bone marrow derived mesenchymal stem cell (BMSC) infiltration within porous scaffolds, where small pores promote horizontal cellular bridging and large pores facilitate vertical migration.
Xiang Gao +15 more
wiley +1 more source
Visitors Without Passports: A Synthesis of Invasion Routes and Phylogeographic Patterns in the Asian Tiger Mosquito Based on Single-Locus Genetic Analyses. [PDF]
Zool StudLondoño-Sanchez JC +3 more
europepmc +1 more source
Wireless Bioelectronic Modulation of Membrane Potential in Glioblastoma Using Carbon Nanotube Porins
Advanced Science, EarlyView.A schematic illustrating glioblastoma cell membrane modulation using carbon nanotube porins (CNTPs) and an applied voltage. CNTPs enable ion flux leading to changes in membrane potential. This figure summarizes the concept of bioelectronic control of cell membrane voltage.
Fleur Groualle +6 more
wiley +1 more source
Strengthening research and training on insecticide resistance in arthropod vectors in South America: The WINSA network. [PDF]
PLoS Negl Trop DisCorbel V +17 more
europepmc +1 more source
Advanced Science, EarlyView.Cryo‐EM analysis reveals an asymmetric MMOH–MMOB complex with distinct protomers, where MMOB binding triggers structural rearrangements from the surface to the active site. This conformational asymmetry shortens the Fe···Fe distance to 2.7 Å in one protomer, forming a geometry suitable for O2 activation and supporting a sequential catalytic mechanism ...
Yunha Hwang +9 more
wiley +1 more source
The epidemiological and clinical differences between patients with mpox and patients without mpox among suspected cases during the recent global mpox outbreaks: a systematic review and meta-analysis. [PDF]
Front Cell Infect MicrobiolHan Y, Li X, Wang X, Zhong Z.
europepmc +1 more source
Droplet Digital CRISPR for Nucleic Acid Detection
Advanced Science, EarlyView.This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang +4 more
wiley +1 more source