Results 111 to 120 of about 937,893 (307)

Nanoscale Spatial Organization of ARC High‐ and Low‐Order Assemblies at Excitatory Synapses

Advanced Science, EarlyView.
ARC (Activity‐Regulated Cytoskeleton‐Associated protein) mediates synaptic plasticity by forming nanoscale assemblies in neurons. Using super‐resolution microscopy and time‐resolved anisotropy with targeted tagging, the study reveals low‐order ARC assemblies at synapses colocalizing with AMPARs, semi‐circular structures at endocytic zones, and 60–80 nm
Martina Damenti, Giovanna Coceano, Mariline Mendes Silva, Jonatan Alvelid, Chiara Sgattoni, Andrea Volpato, Lea Rems, Luciano A. Masullo, Eduard M. Unterauer, Rafal Kowalewski, Lucie Delemotte, Erdinc Sezgin, Ralf Jungmann, Ilaria Testa   +13 more
wiley   +1 more source

HIV controllers: hope for a functional cure

Frontiers in Immunology
Elite controllers (ECs) and post-treatment controllers (PTCs) represent important models for achieving a functional cure for HIV. This review synthesizes findings from immunological, genetic, and virological studies to compare the mechanisms underlying ...
Zhuoya Deng, Zhuoya Deng, Hongxia Yan, Hongxia Yan, Olivier Lambotte, Christiane Moog, Christiane Moog, Bin Su, Bin Su, Bin Su   +9 more
doaj   +1 more source

In vitro Infection of Primary Human Monocytes with HIV-1

Bio-Protocol, 2019
Monocyte infection by HIV-1 is an important component of chronic HIV pathogenesis. Following infection by HIV-1, monocytes are able to cross the blood brain barrier and set up a viral reservoir in the central nervous system.
Patrick O’Connell, Yong-Hui Zheng, Andrea Amalfitano, Yasser Aldhamen   +3 more
doaj   +1 more source

Structural Insights Into Man9 Recognition by the HIV Antibody 2G12 Revealed by Paramagnetic NMR

Advanced Science, EarlyView.
The HIV gp120 glycoprotein displays Man9 oligomannose structures that are recognized by the neutralizing antibody 2G12. Detailed characterization of the binding epitope in solution is important for immunogen design but remains challenging, since the signals of the terminal mannoses from the three arms of Man9 (D1, D2, and D3) cannot be distinguished in
Adrián Silva‐Díaz, Paola Oquist‐Phillips, Noelia de la Cruz, Inés Lera‐Lasso, Laura Castañar, Javier Rojo, Javier Ramos‐Soriano, Ángeles Canales   +7 more
wiley   +1 more source

Exploring the role of multi-pathogen infections in Alzheimer’s disease: A case–control study

Virulence
Background Alzheimer’s disease (AD) is a leading cause of dementia. Following the discovery of the amyloid’s antimicrobial properties, research has focused on infectious agents as potential contributors. This study investigates the role of multi-pathogen
Victor Garcia-Bustos, Marta Dafne Cabanero-Navalon, Carmen Lloret-Sos, MariaDolores Gómez, Miguel Baquero, Santos Ibanez-Barcelo, Javier Grimaldos, Consuelo Cháfer-Pericás   +7 more
doaj   +1 more source

A Biomimetic Lubricant Captures Hyaluronic Acid In Situ to Regenerate Cartilage: From Bench to Bedside

Advanced Science, EarlyView.
(a) The outstanding lubricative properties of natural articular cartilage arise from a composite coating composed of HA, lubricin, and phospholipids at its surface. Notably, lubricin facilitates the enrichment of synovial HA onto cartilage surfaces, and HA complexes with phospholipids, a key component to maintain extremely low friction under high ...
Yongan Lin, Zijian Yan, Jiayi Chen, Xiaochao Wang, Ruibin Lin, Yunqi Fu, Zhaoying Lv, Bohui Wu, Xuewei Cao, Renjian Xie, Ming Dong, Chenxiao Zheng, Li Ren   +12 more
wiley   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Human Vault Nanoparticle Targeted Delivery of Antiretroviral Drugs to Inhibit Human Immunodeficiency Virus Type 1 Infection. [PDF]

, 2019
"Vaults" are ubiquitously expressed endogenous ribonucleoprotein nanoparticles with potential utility for targeted drug delivery. Here, we show that recombinant human vault nanoparticles are readily engulfed by certain key human peripheral blood ...
Anton, Peter A, Deming, Timothy, Elliott, Julie, Fulcher, Jennifer A, Ibarrondo, F Javier, Kickhoefer, Valerie A, Maynard, Heather D, Mrazek, Jan, Rome, Leonard H, Tamshen, Kyle, Wollenberg, Alexander L, Yang, Otto O   +11 more
core  

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, EarlyView.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source