A Novel Gene Synthesis Platform for Designing Functional Protein Polymers
Advanced Science, Volume 12, Issue 15, April 17, 2025.A new gene synthesis method consisting of rolling‐circle amplification and seamless cloning facilitates the advanced design of protein polymers with repetitive sequences. This method is more cost‐effective and user‐friendly than conventional methods. Using this method, a library of polymers with different repeat units can be easily constructed.
Toshimasa Homma +4 more
wiley +1 more source
RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma
Advanced Science, Volume 12, Issue 16, April 24, 2025.RFWD3 is identified as a key regulator of cisplatin (DDP) sensitivity in osteosarcoma using a genome‐wide CRISPR screen. RFWD3 promotes PHGDH ubiquitination, enhancing NAD+‐mediated nucleotide biosynthesis. Knocking out RFWD3 sensitized osteosarcoma cells to DDP.
Wenchao Zhang +6 more
wiley +1 more source
Quantifying Genetic Innovation: Mathematical Foundations for the Topological Study of Reticulate Evolution [PDF]
arXiv, 2018 A topological approach to the study of genetic recombination, based on persistent homology, was introduced by Chan, Carlsson, and Rabad\'an in 2013. This associates a sequence of signatures called barcodes to genomic data sampled from an evolutionary history. In this paper, we develop theoretical foundations for this approach. First, we present a novel
arxiv
RecA + -Dependent Repair of Gamma-Ray Damage to Escherichia coli Does Not Require Recombination Between Existing Homologous Chromosomes [PDF]
, 1971 B.A. Bridges
openalex +1 more source
Targeted recombination between homologous chromosomes for precise breeding in tomato
Nature Communications, 2017 Homologous recombination (HR) between parental chromosomes occurs stochastically. Here, we report on targeted recombination between homologous chromosomes upon somatic induction of DNA double-strand breaks (DSBs) via CRISPR-Cas9.
Shdema Filler Hayut +2 more
semanticscholar +1 more source
Aging‐induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense
Advanced Science, Volume 12, Issue 12, March 27, 2025.Bacterial infections in older individuals often result in worse outcomes, but the mechanisms remain unclear. This study reveals that aging‐induced alterations in gut microbiota impair host antibacterial defense mechanisms, and exacerbate the consequences of bacterial infections.
Peng Gu +20 more
wiley +1 more source
Category theory for genetics II: genotype, phenotype and haplotype [PDF]
arXiv, 2018 The overarching goal of this paper is to solve the word problem for a class of idempotent commutative monoids whose elements model population haplotypes. More specifically, we design an algebraic framework in which it is possible to unravel population stratification relationships and infer linkage disequilibrium in terms of algebraic equations of ...
arxiv
Homologous recombination can restore normal immunoglobulin production in a mutant hybridoma cell line. [PDF]
, 1988 Mark D. Baker +3 more
openalex +1 more source
Biophysics and PhysicobiologyJCVI-syn3B (syn3B), a minimal synthetic bacterium that only possesses essential genes, facilitates the examination of heterogeneous gene functions in minimal life. Conventionally, Escherichia coli is used to construct DNA fragments for gene transfer into
Atsuko Uenoyama +3 more
doaj +1 more source
Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination
Nature Communications, 2018 Homologous recombination ensures genome integrity during meiotic recombination. Here the authors reveal that factors SWS1 and SWSAP1 are critical for meiotic homologues recombination, particularly in promoting assembly of RAD51 and DMC1 on early ...
Carla M. Abreu +5 more
doaj +1 more source