Quantification of gliadin levels to the picogram level by flow cytometry [PDF]
Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of
CAPPARELLI R +3 more
core +1 more source
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine.
Ehab El-Sayed Ibrahim +3 more
doaj +1 more source
Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA) [PDF]
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay.
Avrameas +17 more
core +1 more source
Quantification of Uric Acid or Xanthine in Plant Samples
We developed this protocol to assay and quantify the content of uric acid or xanthine in various tissues of Arabidopsis thaliana mutant lines with defective urate oxidase or xanthine dehydrogenase1 and in their complementation and suppressor lines (Hauck
Oliver Hauck, Claus-Peter Witte
doaj +1 more source
Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]
Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi +8 more
doaj +1 more source
Phagocytic properties of lung alveolar wall cells [PDF]
For the purpose to define the mechanism of heavy metal intoxication by inhalation, morphologic observations were made on rat lungs after nasal instillation of iron colloid particles of positive and negative electric charges. Histochemical observation was
Tanaka, Akisuke
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Compounds I of Catalase and Horse Radish Peroxidase: π-Cation Radicals [PDF]
Two-electron oxidation of cobaltous octaethylporphyrin [Co(II)(Et) 8 P] yields a stable π-cation radical [Co(III)(Et) 8 P] 2+. , the optical spectrum of which exhibits spectral changes dependent upon the nature of the counterion.
D, Dolphin +4 more
openaire +2 more sources
Reversal of age-related learning deficiency by the vertebrate PACAP and IGF-1 in a novel invertebrate model of aging: the pond snail (Lymnaea Stagnalis) [PDF]
With the increase of life span, nonpathological age-related memory decline is affecting an increasing number of people. However, there is evidence that age-associated memory impairment only suspends, rather than irreversibly extinguishes, the intrinsic ...
Alexander +38 more
core +2 more sources
Granulocyte-activating mediators (GRAM) [PDF]
In the present study we investigated the capability of human epidermal cells to generate granulocyte-activating mediators (GRAM). It could be shown that human epidermal cells as well as an epidermoid carcinoma cell line (A431) produce an epidermal cell ...
Kapp, A. +3 more
core +1 more source
TIV vaccination modulates host responses to influenza virus infection that correlate with protection against bacterial superinfection [PDF]
Background: Influenza virus infection predisposes to secondary bacterial pneumonia. Currently licensed influenza vaccines aim at the induction of neutralizing antibodies and are less effective if the induction of neutralizing antibodies is low and/or the
Choi, Angela +4 more
core +2 more sources

