Results 21 to 30 of about 16,901 (208)

HIV-1 establishes immediate latency in T cells expressing the viral Nef protein. [PDF]

FEBS Open Bio
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Lam C, Sadowski I.
europepmc   +2 more sources

PRODUCTION AND CHARACTERIZATION OF THREE BRUCELLA ANTIGENS, LIPOPOL- YSACCHRIDE (LPS), SONICATED CELLS AND WHOLE CELLS ANTIGEN

The Iraqi Journal of Veterinary Medicine, 1996
Three types of Brucella abortus antigens were prepared: Lipopolysaccharide (LPS), sonicated cells and whole cells killed antigens. Enzyme-linked immunosorbent assay (ELSIA) was carried out in polysterene microtiter plates using horse-radish peroxidase ...
NITHAL RAOUF MAHDI
doaj   +1 more source

Cross reactivities of rabbit anti-chicken horse radish peroxidase conjugate with sera of some other avian species in elisa system

, 2009
The cross reactivities of rabbit anti chicken horse radish peroxidase (conjugate) was tested with sera of Chicken, Ducks, Geese, Guinea fowl, Hawks, Pigeons and Turkeys in indirect enzyme linked immunosorbent assay (ELISA) technique.
Owoade, A.A., Fagbohun, O.A., A.A Owoade, D.O Oluwayelu, O.A Fagbohun, Oluwayelu, D.O.   +5 more
core   +2 more sources

H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay

Journal of Innovative Optical Health Sciences, 2022
Photoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases.
Jiayue Zhao, Fei Gong, Nailin Yang, Huali Lei, Zhihui Han, Yuqi Yang, Liang Cheng   +6 more
doaj   +1 more source

SEROLOGICAL DIAGNOSIS OF BRUCELLOSIS IN CATTLE AND SHEEP USING MONOCLONAL ANTIBODIES [PDF]

Assiut Veterinary Medical Journal, 1993
Concentration of monoclonal antibodies raised in mice against Brucella melitensis 16 M has been made. These monoclonal antibodies reacted only with Brucella species and not with other genera of Gram-negative bacteria.
A. ZAGHAWA, S.A. HATTAB, I. GRIESER WILKE, V. MOENNIG   +3 more
doaj   +1 more source

Encapsulation Capacity of β-Cyclodextrin Stabilized Silver Nanoparticles towards Creatinine Enhances the Colorimetric Sensing of Hydrogen Peroxide in Urine

Nanomaterials, 2021
The β-cyclodextrin shell of synthesized silver nanoparticles (βCD-AgNPs) are found to enhance the detection of hydrogen peroxide in urine when compared to the Horse Radish Peroxidase assay kit.
Abdelaziz Elgamouz, Chahlaa Nassab, Alaa Bihi, Somaya A. I. Mohamad, Aisha H. S. A. Almusafri, Salman S. Alharthi, Sarah A. E. Abdulla, Shashikant P. Patole   +7 more
doaj   +1 more source

Immobilization of oxidoreductases on gold and silver nanoparticles

Біологічні студії, 2014
Nanoparticles of silver and gold were synthesized by chemical methods. Nanoparticles of silver were obtained by reducing of silvernitrate with glucose and nanoparticles of gold – by reducing of tetrachloroauric acid trihydrate with sodium citrate or boro­
M. M. Synenka, N. Ye. Stasyuk, T. V. Semashko, G. Z. Gayda, R. V. Mikhailova, M. V. Gonchar   +5 more
doaj   +1 more source

Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA)

Scientific Reports, 2021
This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA).
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang   +3 more
doaj   +1 more source

Magnetic bead-based electrochemical aptasensor doped with multi-wall carbon nanotubes for the detection of ampicillin in milk

International Journal of Electrochemical Science, 2020
Given the ubiquitous presence of antibiotic residues in foodstuff, establishing an efficient method to determine antibiotic residuals is extremely urgent to ensure food safety. This work reports a magnetic bead-based electrochemical aptasensor doped with
Fang Li, Junya Zhu, Yunhong Liu, Zhaozhou Lil, Huaibin Kang, Ruohan Li   +5 more
doaj   +1 more source

Efficacy of Iron Oxide Nanoparticles in Diagnosis of Schistosomiasis [PDF]

Al-Azhar International Medical Journal, 2020
Background: One of the worldwide major public health problems is urinary schistosomiasis that is caused by Schistosoma heamatobium. There are several immunodiagnostic methods used for that diagnosis of such disease, but some are more sensitive and ...
Ibrahim Aly
doaj   +1 more source