Results 161 to 170 of about 468,846 (206)
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Heme-modification studies on horseradish peroxidase
Biochimica et Biophysica Acta (BBA) - Enzymology, 1972Abstract Synthetic horseradish peroxidases (donor: H 2 O 2 oxidoreductase, EC 1.11.1.7) were prepared from apoenzyme and modified heme. The synthetic enzymes containing proto-, meso- or deuteroheme or protoheme monomethyl ester reacted with stoichiometric amounts of hydroperoxides, to form Compound I with these synthetic enzymes. Other hydroperoxide-
M, Tamura, T, Asakura, T, Yonetani
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Phenols removal by immobilized horseradish peroxidase
Journal of Hazardous Materials, 2009Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride.
I, Alemzadeh, S, Nejati
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Calcium-related properties of horseradish peroxidase
Biochemical and Biophysical Research Communications, 1978Abstract Horseradish peroxidase has been shown to be a metalloprotein in which calcium contributes to the structural stability of the protein. Isoenzyme C and A contain 2.0 and 1.4 moles calcium/mole enzyme, respectively, which can be removed by treatment with guanidine hydrochloride and EDTA.
R H, Haschke, J M, Friedhoff
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Thermostabilized chemical derivatives of horseradish peroxidase
Enzyme and Microbial Technology, 1994Horseradish peroxidase finds a variety of uses in analysis, immunology, organic synthesis, and biosensors. Although moderately stable, its applicability to biosensors and other fields would be greatly enhanced if it could be made yet more stable. Appropriate chemical modification can substantially stabilize enzymes.
O, Ryan, M R, Smyth, C O, Fágáin
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Horseradish peroxidase-catalyzed hydroxylations: mechanistic studies
Biochemistry, 1986The hydroxylation of phenol to hydroquinone and catechol in the presence of dihydroxyfumaric acid and oxygen catalyzed by horseradish peroxidase was studied under conditions where the product yield was high and the side reactions were minimal. The reaction is partially uncoupled with a molar ratio of dihydroxyfumaric acid consumed to hydroxylated ...
J S, Dordick +2 more
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Oxidative Degradation of Alkylphenols by Horseradish Peroxidase
Journal of Bioscience and Bioengineering, 2003Alkylphenols such as bisphenol A (2,2-bis(4-hydroxyphenyl)propane; BPA), p-nonylphenol (p-NP), and p-octylphenol (p-OP) that are known as endocrine disrupters were oxidized by horseradish (Armoracia rusticana) peroxidase (HRP) with H2O2. The optimal pHs for BPA, p-NP, and p-OP were 8.0, 7.0, and 5.0, respectively. The optimal temperature for BPA was 20
Hisae, Sakuyama +3 more
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Photoinduced Oxidation of Horseradish Peroxidase
Journal of the American Chemical Society, 1997Photoexcited [Ru(bpy)3]2+ (bpy = 2,2‘-bipyridine) can be quenched with [Co(NH3)5Cl]2+ to give [Ru(bpy)3]3+; this photogenerated oxidant (E0 = 1.25 V vs NHE) reacts with horseradish peroxidase isoenzyme c (HRPc) to produce oxidized protein species. Spectra and kinetics measured by laser-flash transient spectroscopy show that oxidation of the trivalent ...
Johan Berglund +3 more
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Alkylation of horseradish peroxidase with lodoacetate
Archives of Biochemistry and Biophysics, 1968Abstract A kinetic and chemical study of the inactivation of horseradish peroxidase by reaction with iodoacetate has been undertaken to gain insight into the chemical behavior and reactivity of the protein moiety with a view toward fully defining the catalytic site of the enzyme.
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[Stabilizers of horseradish peroxidase].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010Keeping an enzyme in its native form with high catalytic activity is of great significance. In the present study, thermal stabilizers of horseradish peroxidase (HRP) were screened. The results indicated that thermal stability of HRP was enhanced by magnesium sulphate and gelatin. A synergic effect of magnesium sulphate and gelatin was observed.
Xinhuan, Mao +4 more
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